################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccII AccIII AclI AfaI AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoT22I FbaI FseI FspI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 2320 GACGTC GACGT^C AcyI 2320 GACGTC G[GA]^CG[CT]C AhdI 41 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 838 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 2753 AGCGCT AGC^GCT ApaI 458 GGGCCC GGGCC^C Asp700I 318 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 41 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1984 GGATCC G^GATCC BcgI 534 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 2320 GACGTC G[GA]^CG[CT]C BsaI 513 GAGACC GGTCTC(1/5) BsgI 723 GTGCAG GTGCAG(16/14) BsmI 439 GCATTC GAATGC(1/-1) BspDI 1978 ATCGAT AT^CGAT BssSI 1120 CACGAG CACGAG(-5/-1) BstXI 578 CCATTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 2754 GCGC GCG^C ClaI 1978 ATCGAT AT^CGAT Eam1105I 41 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2753 AGCGCT AGC^GCT EcoRI 1878 GAATTC G^AATTC EcoRV 2591 GATATC GAT^ATC HaeII 2753 AGCGCT [GA]GCGC^[CT] HhaI 2754 GCGC GCG^C Hin1I 2320 GACGTC G[GA]^CG[CT]C HinP1I 2754 GCGC G^CGC HindIII 2632 AAGCTT A^AGCTT HpaI 1129 GTTAAC GTT^AAC MfeI 1958 CAATTG C^AATTG MunI 1958 CAATTG C^AATTG PaeR7I 2075 CTCGAG C^TCGAG PpuMI 1430 AGGACCT [GA]G^G[AT]CC[CT] PshAI 262 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1366 CAGCTG CAG^CTG SmaI 1448 CCCGGG CCC^GGG XbaI 2511 TCTAGA T^CTAGA XhoI 2075 CTCGAG C^TCGAG XmaCI 1448 CCCGGG C^CCGGG XmaI 1448 CCCGGG C^CCGGG XmnI 318 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 595 GTAGAC GT^[AC][GT]AC 2622 GTCTAC AcsI 1878 GAATTC [GA]^AATT[CT] 2007 GAATTT ApoI 1878 GAATTC [GA]^AATT[CT] 2007 GAATTT AspI 640 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1963 GACCTGGTC BalI 413 TGGCCA TGG^CCA 656 TGGCCA BanI 397 GGCACC G^G[CT][GA]CC 2274 GGTGCC BsmBI 2322 CGTCTC CGTCTC(1/5) 676 GAGACG BspLU11I 719 ACATGT A^CATGT 2219 ACATGT BsrBI 2072 CCGCTC CCGCTC(-3/-3) 73 GAGCGG BstAPI 1313 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1937 GCAAGAAATGC FauI 123 CCCGC CCCGC(4/6) 162 GCGGG HgaI 678 GACGC GACGC(5/10) 1250 GCGTC HphI 278 TCACC GGTGA(8/7) 2297 TCACC MluNI 413 TGGCCA TGG^CCA 656 TGGCCA MscI 413 TGGCCA TGG^CCA 656 TGGCCA MspA1I 1278 CCGCTG C[AC]G^C[GT]G 1366 CAGCTG NspI 719 ACATGT [GA]CATG^[CT] 2219 ACATGT PflFI 640 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1963 GACCTGGTC SexAI 170 ACCTGGT A^CC[AT]GGT 1964 ACCTGGT TaqI 1979 TCGA T^CGA 2076 TCGA Tth111I 640 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1963 GACCTGGTC TthHB8I 1979 TCGA T^CGA 2076 TCGA XcmI 401 CCATGACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 909 CCAGTGCTACAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AflIII AluI Alw44I AlwI AocI ApaLI AspHI AvaI AvaII BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PmaCI PmlI PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII