(Circular) (Six-base) MAPSORT of: pF1KB8523.seq Check: 3516 from: 1 to: 5924 pF1KB8523 5924 bp With 182 enzymes: SgfI * October 7, 2009 14:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 884 884 Size: 5924 AatII G_ACGT'C Cuts at: 2432 2432 Size: 5924 AccI GT'mk_AC Cuts at: 704 2731 2962 704 Size: 2027 231 3666 Fragments arranged by size: 3666 2027 231 Acc65I G'GTAC_C Cuts at: 2940 2940 Size: 5924 AclI AA'CG_TT Cuts at: 5764 5764 Size: 5924 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2078 2747 3749 4181 4928 5196 2078 Size: 669 1002 432 747 268 2806 Fragments arranged by size: 2806 1002 747 669 432 268 AfeI AGC'GCT Cuts at: 2863 2863 Size: 5924 AflIII A'CryG_T Cuts at: 827 1647 1672 1871 2327 3276 4380 5357 827 Size: 820 25 199 456 949 1104 977 1394 Fragments arranged by size: 1394 1104 977 949 820 456 199 25 AgeI A'CCGG_T Cuts at: 4312 4312 Size: 5924 AhdI GACnn_n'nnGTC Cuts at: 154 154 Size: 5924 AleI CACnn'nnGTG Cuts at: 391 974 1097 1232 1712 1847 1970 2380 391 Size: 583 123 135 480 135 123 410 3935 Fragments arranged by size: 3935 583 480 410 135 135 123 123 AlwNI CAG_nnn'CTG Cuts at: 951 3229 4796 951 Size: 2278 1567 2079 Fragments arranged by size: 2278 2079 1567 ApaI G_GGCC'C Cuts at: 570 570 Size: 5924 ApaLI G'TGCA_C Cuts at: 816 1182 1325 1448 4694 816 Size: 366 143 123 3246 2046 Fragments arranged by size: 3246 2046 366 143 123 ApoI r'AATT_y Cuts at: 1986 2115 2928 5277 5533 1986 Size: 129 813 2349 256 2377 Fragments arranged by size: 2377 2349 813 256 129 AseI AT'TA_AT Cuts at: 20 20 Size: 5924 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5924 AvaI C'yCGr_G Cuts at: 389 1556 2183 2944 389 Size: 1167 627 761 3369 Fragments arranged by size: 3369 1167 761 627 BamHI G'GATC_C Cuts at: 2092 2949 2092 Size: 857 5067 BanI G'GyrC_C Cuts at: 505 2382 2940 3354 3602 3637 505 Size: 1877 558 414 248 35 2792 Fragments arranged by size: 2792 1877 558 414 248 35 BanII G_rGCy'C Cuts at: 570 740 1006 2302 2421 2534 2938 3968 570 Size: 170 266 1296 119 113 404 1030 2526 Fragments arranged by size: 2526 1296 1030 404 266 170 119 113 BbeI G_GCGC'C Cuts at: 3358 3606 3358 Size: 248 5676 BbsI GAAGACnn'nnnn_ Cuts at: 747 1675 2043 2630 2643 2720 747 Size: 928 368 587 13 77 3951 Fragments arranged by size: 3951 928 587 368 77 13 BbvCI CC'TCA_GC Cuts at: 1043 1904 2027 1043 Size: 861 123 4940 Fragments arranged by size: 4940 861 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 631 665 631 Size: 34 5890 BciVI GTATCCnnnnn_n' Cuts at: 3822 4583 5924 3822 Size: 761 1341 3822 Fragments arranged by size: 3822 1341 761 BclI T'GATC_A Cuts at: 3444 3444 Size: 5924 BglI GCCn_nnn'nGGC Cuts at: 646 1274 1397 646 Size: 628 123 5173 Fragments arranged by size: 5173 628 123 BglII A'GATC_T Cuts at: 3439 5476 3439 Size: 2037 3887 BlpI GC'TnA_GC Cuts at: 3036 3036 Size: 5924 Bme1580I G_kGCm'C Cuts at: 508 570 820 858 1186 1329 1452 2387 3549 Size: 62 250 38 328 143 123 935 1162 Cuts at: 3549 3642 4698 508 Size: 93 1056 1734 Fragments arranged by size: 1734 1162 1056 935 328 250 143 123 93 62 38 BmgBI CAC'GTC Cuts at: 1673 1673 Size: 5924 BmrI ACTGGGnnnn_n' Cuts at: 524 2318 3136 3217 3552 5696 524 Size: 1794 818 81 335 2144 752 Fragments arranged by size: 2144 1794 818 752 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2541 4291 2541 Size: 1750 4174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 268 2182 2633 268 Size: 1914 451 3559 Fragments arranged by size: 3559 1914 451 Bpu10I CC'TnA_GC Cuts at: 653 1043 1904 2027 2420 653 Size: 390 861 123 393 4157 Fragments arranged by size: 4157 861 393 390 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 424 1528 2581 3100 3979 4471 4769 424 Size: 1104 1053 519 879 492 298 1579 Fragments arranged by size: 1579 1104 1053 879 519 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 615 5628 615 Size: 5013 911 BsaAI yAC'GTr Cuts at: 190 1650 1874 3277 190 Size: 1460 224 1403 2837 Fragments arranged by size: 2837 1460 1403 224 BsaBI GATnn'nnATC Cuts at: 6 3438 5475 6 Size: 3432 2037 455 Fragments arranged by size: 3432 2037 455 BsaHI Gr'CG_yC Cuts at: 2429 3355 3603 4305 5801 2429 Size: 926 248 702 1496 2552 Fragments arranged by size: 2552 1496 926 702 248 BsaWI w'CCGG_w Cuts at: 3113 3634 4312 4586 4733 5144 3113 Size: 521 678 274 147 411 3893 Fragments arranged by size: 3893 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1637 1667 1637 Size: 30 5894 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 151 1384 2518 151 Size: 1233 1134 3557 Fragments arranged by size: 3557 1233 1134 BseYI C'CCAG_C Cuts at: 3349 4107 4684 5180 3349 Size: 758 577 496 4093 Fragments arranged by size: 4093 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 852 852 Size: 5924 BsiEI CG_ry'CG Cuts at: 104 3466 3512 4720 104 Size: 3362 46 1208 1308 Fragments arranged by size: 3362 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 820 1186 1329 1452 1516 1655 1698 2938 3716 Size: 366 143 123 64 139 43 1240 778 Cuts at: 3716 3906 4698 820 Size: 190 792 2046 Fragments arranged by size: 2046 1240 792 778 366 190 143 139 123 64 43 BsmI GAATG_Cn' Cuts at: 547 547 Size: 5924 BsmBI CGTCTCn'nnnn_ Cuts at: 778 2436 778 Size: 1658 4266 Bsp1286I G_dGCh'C Cuts at: 508 570 740 820 858 1006 1186 1329 1452 Size: 62 170 80 38 148 180 143 123 Cuts at: 1452 1516 1655 1698 2302 2387 2421 2534 2938 Size: 64 139 43 604 85 34 113 404 Cuts at: 2938 3549 3642 3716 3906 3968 4698 508 Size: 611 93 74 190 62 730 1734 Fragments arranged by size: 1734 730 611 604 404 190 180 170 148 143 139 123 113 93 85 80 74 64 62 62 43 38 34 BspEI T'CCGG_A Cuts at: 5144 5144 Size: 5924 BspHI T'CATG_A Cuts at: 5100 5100 Size: 5924 BspMI ACCTGCnnnn'nnnn_ Cuts at: 884 1312 1435 1558 1804 2974 3188 3490 3871 Size: 428 123 123 246 1170 214 302 381 Cuts at: 3871 884 Size: 2937 Fragments arranged by size: 2937 1170 428 381 302 246 214 123 123 BsrBI CCG'CTC Cuts at: 50 183 2182 4216 4270 5754 5919 50 Size: 133 1999 2034 54 1484 165 55 Fragments arranged by size: 2034 1999 1484 165 133 55 54 BsrDI GCAATG_nn' Cuts at: 3836 3836 Size: 5924 BsrFI r'CCGG_y Cuts at: 3922 4103 4312 3922 Size: 181 209 5534 Fragments arranged by size: 5534 209 181 BssSI C'ACGA_G Cuts at: 1228 4195 4553 1228 Size: 2967 358 2599 Fragments arranged by size: 2967 2599 358 BstAPI GCAn_nnn'nTGC Cuts at: 1427 2051 3301 1427 Size: 624 1250 4050 Fragments arranged by size: 4050 1250 624 BstBI TT'CG_AA Cuts at: 4285 4285 Size: 5924 BstXI CCAn_nnnn'nTGG Cuts at: 693 693 Size: 5924 BstYI r'GATC_y Cuts at: 1 948 1618 2092 2949 3416 3439 3774 4020 Size: 947 670 474 857 467 23 335 246 Cuts at: 4020 5032 5118 5130 5476 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 947 857 670 474 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 577 626 1640 577 Size: 49 1014 4861 Fragments arranged by size: 4861 1014 49 BtgI C'CryG_G Cuts at: 107 500 592 635 959 1092 1395 1461 1584 Size: 393 92 43 324 133 303 66 123 Cuts at: 1584 1707 1830 1953 2270 107 Size: 123 123 123 317 3761 Fragments arranged by size: 3761 393 324 317 303 133 123 123 123 123 92 66 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 528 1676 3874 4055 4218 528 Size: 1148 2198 181 163 2234 Fragments arranged by size: 2234 2198 1148 181 163 BtsI GCAGTG_nn' Cuts at: 312 1263 1740 3167 312 Size: 951 477 1427 3069 Fragments arranged by size: 3069 1427 951 477 ClaI AT'CG_AT Cuts at: 2087 2087 Size: 5924 DraI TTT'AAA Cuts at: 2923 2923 Size: 5924 DrdI GACnn_nn'nnGTC Cuts at: 3630 4488 3630 Size: 858 5066 EaeI y'GGCC_r Cuts at: 521 638 764 3509 3683 4074 4101 5446 521 Size: 117 126 2745 174 391 27 1345 999 Fragments arranged by size: 2745 1345 999 391 174 126 117 27 EagI C'GGCC_G Cuts at: 3509 3509 Size: 5924 EarI CTCTTCn'nnn_ Cuts at: 1048 2105 2554 3947 4157 1048 Size: 1057 449 1393 210 2815 Fragments arranged by size: 2815 1393 1057 449 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4132 4442 4588 5734 4132 Size: 310 146 1146 4322 Fragments arranged by size: 4322 1146 310 146 EcoICRI GAG'CTC Cuts at: 2936 2936 Size: 5924 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 268 2078 2182 2633 2747 3749 4181 4928 5196 Size: 1810 104 451 114 1002 432 747 268 Cuts at: 5196 268 Size: 996 Fragments arranged by size: 1810 1002 996 747 451 432 268 114 104 EcoNI CCTnn'n_nnAGG Cuts at: 450 1042 1308 1800 450 Size: 592 266 492 4574 Fragments arranged by size: 4574 592 492 266 EcoO109I rG'GnC_Cy Cuts at: 566 1539 2366 3063 566 Size: 973 827 697 3427 Fragments arranged by size: 3427 973 827 697 EcoRI G'AATT_C Cuts at: 1986 2928 1986 Size: 942 4982 EcoRV GAT'ATC Cuts at: 2701 2701 Size: 5924 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2565 2737 3943 2565 Size: 172 1206 4546 Fragments arranged by size: 4546 1206 172 FspI TGC'GCA Cuts at: 3705 3705 Size: 5924 HaeII r_GCGC'y Cuts at: 2865 3358 3606 4628 5607 2865 Size: 493 248 1022 979 3182 Fragments arranged by size: 3182 1022 979 493 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 140 172 477 509 1637 1669 1883 1915 2416 Size: 32 305 32 1128 32 214 32 501 Cuts at: 2416 2448 3766 3796 3798 3828 140 Size: 32 1318 30 2 30 2236 Fragments arranged by size: 2236 1318 1128 501 305 214 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 312 1239 1362 1608 2963 312 Size: 927 123 246 1355 3273 Fragments arranged by size: 3273 1355 927 246 123 HindIII A'AGCT_T Cuts at: 2740 2740 Size: 5924 HpaI GTT'AAC Cuts at: 1239 1239 Size: 5924 KasI G'GCGC_C Cuts at: 3354 3602 3354 Size: 248 5676 KpnI G_GTAC'C Cuts at: 2944 2944 Size: 5924 MfeI C'AATT_G Cuts at: 2066 2066 Size: 5924 MluI A'CGCG_T Cuts at: 5357 5357 Size: 5924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2700 4595 4779 2700 Size: 1895 184 3845 Fragments arranged by size: 3845 1895 184 MscI TGG'CCA Cuts at: 523 766 3685 523 Size: 243 2919 2762 Fragments arranged by size: 2919 2762 243 MslI CAynn'nnrTG Cuts at: 323 391 691 851 974 1097 1109 1232 1466 Size: 68 300 160 123 123 12 123 234 Cuts at: 1466 1589 1712 1724 1835 1847 1849 1872 1970 Size: 123 123 12 111 12 2 23 98 Cuts at: 1970 2380 3913 4040 323 Size: 410 1533 127 2207 Fragments arranged by size: 2207 1533 410 300 234 160 127 123 123 123 123 123 111 98 68 23 12 12 12 2 MspA1I CmG'CkG Cuts at: 1388 1476 3035 3349 3709 4722 4967 1388 Size: 88 1559 314 360 1013 245 2345 Fragments arranged by size: 2345 1559 1013 360 314 245 88 NaeI GCC'GGC Cuts at: 4105 4105 Size: 5924 NarI GG'CG_CC Cuts at: 3355 3603 3355 Size: 248 5676 NcoI C'CATG_G Cuts at: 107 959 1461 1584 1830 1953 107 Size: 852 502 123 246 123 4078 Fragments arranged by size: 4078 852 502 246 123 123 NgoMIV G'CCGG_C Cuts at: 4103 4103 Size: 5924 NspI r_CATG'y Cuts at: 831 2331 2977 3477 4384 831 Size: 1500 646 500 907 2371 Fragments arranged by size: 2371 1500 907 646 500 PciI A'CATG_T Cuts at: 827 2327 4380 827 Size: 1500 2053 2371 Fragments arranged by size: 2371 2053 1500 PfoI T'CCnGG_A Cuts at: 944 944 Size: 5924 PmeI GTTT'AAAC Cuts at: 2923 2923 Size: 5924 PmlI CAC'GTG Cuts at: 190 1650 1874 190 Size: 1460 224 4240 Fragments arranged by size: 4240 1460 224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5063 5095 5063 Size: 32 5892 PpuMI rG'GwC_Cy Cuts at: 1539 1539 Size: 5924 PshAI GACnn'nnGTC Cuts at: 374 374 Size: 5924 PsiI TTA'TAA Cuts at: 2815 2815 Size: 5924 PspOMI G'GGCC_C Cuts at: 566 566 Size: 5924 PspXI vC'TCGA_Gb Cuts at: 2183 2183 Size: 5924 PstI C_TGCA'G Cuts at: 895 1309 1432 1801 2971 3656 895 Size: 414 123 369 1170 685 3163 Fragments arranged by size: 3163 1170 685 414 369 123 PvuI CG_AT'CG Cuts at: 104 104 Size: 5924 PvuII CAG'CTG Cuts at: 1476 3349 3709 1476 Size: 1873 360 3691 Fragments arranged by size: 3691 1873 360 RsrII CG'GwC_CG Cuts at: 4119 4119 Size: 5924 SacI G_AGCT'C Cuts at: 2938 2938 Size: 5924 SalI G'TCGA_C Cuts at: 2961 2961 Size: 5924 SapI GCTCTTCn'nnn_ Cuts at: 3947 4157 3947 Size: 210 5714 SbfI CC_TGCA'GG Cuts at: 2971 2971 Size: 5924 ScaI AGT'ACT Cuts at: 5441 5441 Size: 5924 SexAI A'CCwGG_T Cuts at: 278 2072 278 Size: 1794 4130 SfcI C'TryA_G Cuts at: 33 891 1023 1305 1428 1797 1823 2967 3652 Size: 858 132 282 123 369 26 1144 685 Cuts at: 3652 4645 4836 33 Size: 993 191 1121 Fragments arranged by size: 1144 1121 993 858 685 369 282 191 132 123 26 SfoI GGC'GCC Cuts at: 3356 3604 3356 Size: 248 5676 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5924 SmaI CCC'GGG Cuts at: 1558 2946 1558 Size: 1388 4536 SmlI C'TyrA_G Cuts at: 439 1543 2183 2596 3079 3994 4486 4748 439 Size: 1104 640 413 483 915 492 262 1615 Fragments arranged by size: 1615 1104 915 640 492 483 413 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5924 SphI G_CATG'C Cuts at: 2977 3477 2977 Size: 500 5424 StyI C'CwwG_G Cuts at: 107 959 1461 1584 1830 1953 2349 2453 3058 Size: 852 502 123 246 123 396 104 605 Cuts at: 3058 3412 107 Size: 354 2619 Fragments arranged by size: 2619 852 605 502 396 354 246 123 123 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 331 3507 3640 4099 4308 331 Size: 3176 133 459 209 1947 Fragments arranged by size: 3176 1947 459 209 133 TatI w'GTAC_w Cuts at: 5439 5439 Size: 5924 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1044 1076 1659 1691 5441 5473 1044 Size: 32 583 32 3750 32 1495 Fragments arranged by size: 3750 1495 583 32 32 32 Tth111I GACn'n_nGTC Cuts at: 751 2074 3721 751 Size: 1323 1647 2954 Fragments arranged by size: 2954 1647 1323 XbaI T'CTAG_A Cuts at: 2619 2955 2619 Size: 336 5588 XcmI CCAnnnn_n'nnnnTGG Cuts at: 516 1024 3419 516 Size: 508 2395 3021 Fragments arranged by size: 3021 2395 508 XhoI C'TCGA_G Cuts at: 2183 2183 Size: 5924 XmaI C'CCGG_G Cuts at: 1556 2944 1556 Size: 1388 4536 XmnI GAAnn'nnTTC Cuts at: 430 430 Size: 5924 ZraI GAC'GTC Cuts at: 2430 2430 Size: 5924 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AflII AlfI AloI AscI AvrII BaeI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI NdeI NheI NotI NruI NsiI PacI PasI PflMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI