Restriction map for pF1KB8496
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwNI       
Aor51HI     ApaI        AscI        AspEI       AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KspI        MluI        MroI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        429  GGTACC               G^GTACC
Alw44I        323  GTGCAC               G^TGCAC
ApaLI         323  GTGCAC               G^TGCAC
AseI          370  ATTAAT               AT^TAAT
AsnI          370  ATTAAT               AT^TAAT
Asp718I       429  GGTACC               G^GTACC
AspI         2009  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          623  TGGCCA               TGG^CCA
BcgI         1176  GCAGCAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1133  GTATCC               GTATCC(6/5)
BsaBI         132  GATTCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI        1409  CCGCTC               CCGCTC(-3/-3)
BstAPI       1743  GCAAATCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI         2569  GCGC                 GCG^C
DraIII       1400  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2456  CCATGG               C^C[GA][CT]GG
EcoRI        1528  GAATTC               G^AATTC
HhaI         2569  GCGC                 GCG^C
HinP1I       2569  GCGC                 G^CGC
HincII       2399  GTCAAC               GT[CT]^[GA]AC
HindII       2399  GTCAAC               GT[CT]^[GA]AC
KpnI          429  GGTACC               GGTAC^C
MamI          132  GATTCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1952  CAATTG               C^AATTG
MluNI         623  TGGCCA               TGG^CCA
MscI          623  TGGCCA               TGG^CCA
MseI          371  TTAA                 T^TAA
MunI         1952  CAATTG               C^AATTG
NcoI         2456  CCATGG               C^CATGG
PaeR7I        251  CTCGAG               C^TCGAG
PflFI        2009  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2247  CCACTCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2071  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI         370  ATTAAT               AT^TAAT
SacI         1861  GAGCTC               GAGCT^C
SspI          333  AATATT               AAT^ATT
StuI         2093  AGGCCT               AGG^CCT
Tru9I         371  TTAA                 T^TAA
Tth111I      2009  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2247  CCACTCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          698  CCAGAAAGAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          251  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        618  ACATGT               A^C[GA][CT]GT
             2403  ACATGT              
AocI         1831  CCTCAGG              CC^T[ACGT]AGG
             2067  CCTAAGG             
Asp700I       486  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1142  GAAAGAGTTC          
AvaI          251  CTCGAG               C^[CT]CG[GA]G
             1820  CCCGAG              
BsaI          379  GAGACC               GGTCTC(1/5)
             2452  GAGACC              
BsaWI        1576  ACCGGA               [AT]^CCGG[AT]
             1597  TCCGGT              
BsoBI         251  CTCGAG               C^[CT]CG[GA]G
             1820  CCCGAG              
Bsp1407I     1589  TGTACA               T^GTACA
             1898  TGTACA              
BspHI        1632  TCATGA               T^CATGA
             1985  TCATGA              
BspLU11I      618  ACATGT               A^CATGT
             2403  ACATGT              
BspMI        1325  ACCTGC               ACCTGC(4/8)
             1970  ACCTGC              
BsrGI        1589  TGTACA               T^GTACA
             1898  TGTACA              
BstBI         214  TTCGAA               TT^CGAA
              972  TTCGAA              
Bsu36I       1831  CCTCAGG              CC^T[ACGT]AGG
             2067  CCTAAGG             
DraII          55  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             2071  AGGTCCC             
Eco81I       1831  CCTCAGG              CC^T[ACGT]AGG
             2067  CCTAAGG             
EcoO109I       55  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             2071  AGGTCCC             
MspA1I       2053  CAGCTG               C[AC]G^C[GT]G
             2116  CAGCTG              
NspV          214  TTCGAA               TT^CGAA
              972  TTCGAA              
PvuII        2053  CAGCTG               CAG^CTG
             2116  CAGCTG              
RcaI         1632  TCATGA               T^CATGA
             1985  TCATGA              
SfuI          214  TTCGAA               TT^CGAA
              972  TTCGAA              
SspBI        1589  TGTACA               T^GTACA
             1898  TGTACA              
XmnI          486  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1142  GAAAGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoNI       EcoO65I     EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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