Restriction map for pF1KB8496
 (Linear) (Six-base) MAPSORT of: KIBB8496.seq  Check: 5263  from: 1  to: 2583



KIBB8496.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

Acc65I G'GTAC_C

Cuts at:      0     429    2583 
   Size:        429    2154
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43    1019    2583 
   Size:         43     976    1564

  Fragments arranged by size: 

                1564     976      43
 
AflIII A'CryG_T

Cuts at:      0     618    2403    2583 
   Size:        618    1785     180

  Fragments arranged by size: 

                1785     618     180
 
AleI CACnn'nnGTG

Cuts at:      0    1366    2160    2583 
   Size:       1366     794     423

  Fragments arranged by size: 

                1366     794     423
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     570    1508    2178    2583 
   Size:        570     938     670     405

  Fragments arranged by size: 

                 938     670     570     405
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1514    1546    1984    2016    2312    2344    2583 
   Size:       1514      32     438      32     296      32     239

  Fragments arranged by size: 

                1514     438     296     239      32      32      32
 
ApaLI G'TGCA_C

Cuts at:      0     323    2583 
   Size:        323    2260
 
ApoI r'AATT_y

Cuts at:      0     490    1528    2304    2583 
   Size:        490    1038     776     279

  Fragments arranged by size: 

                1038     776     490     279
 
AseI AT'TA_AT

Cuts at:      0     371    2583 
   Size:        371    2212
 
AvaI C'yCGr_G

Cuts at:      0     251    1820    2583 
   Size:        251    1569     763

  Fragments arranged by size: 

                1569     763     251
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2419    2452    2583 
   Size:       2419      33     131

  Fragments arranged by size: 

                2419     131      33
 
BanI G'GyrC_C

Cuts at:      0     429    1041    1368    2162    2583 
   Size:        429     612     327     794     421

  Fragments arranged by size: 

                 794     612     429     421     327
 
BanII G_rGCy'C

Cuts at:      0     598    1727    1865    2482    2507    2583 
   Size:        598    1129     138     617      25      76

  Fragments arranged by size: 

                1129     617     598     138      76      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      31     460    1624    2583 
   Size:         31     429    1164     959

  Fragments arranged by size: 

                1164     959     429      31
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1165    1199    2583 
   Size:       1165      34    1384

  Fragments arranged by size: 

                1384    1165      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1144    2583 
   Size:       1144    1439
 
BglII A'GATC_T

Cuts at:      0     829    1030    2192    2583 
   Size:        829     201    1162     391

  Fragments arranged by size: 

                1162     829     391     201
 
Bme1580I G_kGCm'C

Cuts at:      0     327    2583 
   Size:        327    2256
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2020    2583 
   Size:       2020     563
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     831    2359    2583 
   Size:        831    1528     224

  Fragments arranged by size: 

                1528     831     224
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     758     845    1483    2168    2389    2583 
   Size:        730      28      87     638     685     221     194

  Fragments arranged by size: 

                 730     685     638     221     194      87      28
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226    1369    2065    2583 
   Size:        226    1143     696     518

  Fragments arranged by size: 

                1143     696     518     226
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     373    2446    2583 
   Size:        373    2073     137

  Fragments arranged by size: 

                2073     373     137
 
BsaBI GATnn'nnATC

Cuts at:      0     136    2583 
   Size:        136    2447
 
BsaWI w'CCGG_w

Cuts at:      0    1576    1597    2583 
   Size:       1576      21     986

  Fragments arranged by size: 

                1576     986      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     637     667     843     873    1107    1137    2464    2494 
   Size:        637      30     176      30     234      30    1327      30

Cuts at:   2494    2583 
   Size:         89

  Fragments arranged by size: 

                1327     637     234     176      89      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     224    1009    1403    2583 
   Size:        224     785     394    1180

  Fragments arranged by size: 

                1180     785     394     224
 
BseYI C'CCAG_C

Cuts at:      0     609     743    2207    2506    2583 
   Size:        609     134    1464     299      77

  Fragments arranged by size: 

                1464     609     299     134      77
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     566     608    1800    2436    2583 
   Size:         61     505      42    1192     636     147

  Fragments arranged by size: 

                1192     636     505     147      61      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     252     327    1305    1865    2583 
   Size:        252      75     978     560     718

  Fragments arranged by size: 

                 978     718     560     252      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     252     327     598    1305    1727    1865    2482    2507 
   Size:        252      75     271     707     422     138     617      25

Cuts at:   2507    2583 
   Size:         76

  Fragments arranged by size: 

                 707     617     422     271     252     138      76      75
                  25
 
BspHI T'CATG_A

Cuts at:      0    1632    1985    2583 
   Size:       1632     353     598

  Fragments arranged by size: 

                1632     598     353
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1334    1979    2583 
   Size:       1334     645     604

  Fragments arranged by size: 

                1334     645     604
 
BsrBI CCG'CTC

Cuts at:      0    1411    2583 
   Size:       1411    1172
 
BsrGI T'GTAC_A

Cuts at:      0    1589    1898    2583 
   Size:       1589     309     685

  Fragments arranged by size: 

                1589     685     309
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1749    2583 
   Size:       1749     834
 
BstBI TT'CG_AA

Cuts at:      0     215     973    2583 
   Size:        215     758    1610

  Fragments arranged by size: 

                1610     758     215
 
BstEII G'GTnAC_C

Cuts at:      0     727    1826    2235    2583 
   Size:        727    1099     409     348

  Fragments arranged by size: 

                1099     727     409     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1461    1965    2010    2118    2583 
   Size:       1461     504      45     108     465

  Fragments arranged by size: 

                1461     504     465     108      45
 
BstYI r'GATC_y

Cuts at:      0     829    1030    1418    2063    2192    2583 
   Size:        829     201     388     645     129     391

  Fragments arranged by size: 

                 829     645     391     388     201     129
 
Bsu36I CC'TnA_GG

Cuts at:      0    1832    2068    2583 
   Size:       1832     236     515

  Fragments arranged by size: 

                1832     515     236
 
BtgI C'CryG_G

Cuts at:      0    2456    2583 
   Size:       2456     127
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     925    2583 
   Size:        925    1658
 
BtsI GCAGTG_nn'

Cuts at:      0    1266    2583 
   Size:       1266    1317
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     407     442    2583 
   Size:        407      35    2141

  Fragments arranged by size: 

                2141     407      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1405    2583 
   Size:       1405    1178
 
EaeI y'GGCC_r

Cuts at:      0     623    2294    2459    2583 
   Size:        623    1671     165     124

  Fragments arranged by size: 

                1671     623     165     124
 
EarI CTCTTCn'nnn_

Cuts at:      0    1211    1265    1372    1514    2583 
   Size:       1211      54     107     142    1069

  Fragments arranged by size: 

                1211    1069     142     107      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     639    2583 
   Size:        639    1944
 
EcoICRI GAG'CTC

Cuts at:      0    1863    2583 
   Size:       1863     720
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     730     758     845    1019    1483    2168    2389 
   Size:         43     687      28      87     174     464     685     221

Cuts at:   2389    2583 
   Size:        194

  Fragments arranged by size: 

                 687     685     464     221     194     174      87      43
                  28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1363    1610    1654    2583 
   Size:       1363     247      44     929

  Fragments arranged by size: 

                1363     929     247      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0      56    2072    2583 
   Size:         56    2016     511

  Fragments arranged by size: 

                2016     511      56
 
EcoRI G'AATT_C

Cuts at:      0    1528    2583 
   Size:       1528    1055
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     477    1365    2583 
   Size:        477     888    1218

  Fragments arranged by size: 

                1218     888     477
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1107    1139    1149    1181    1410    1442    2382    2414 
   Size:       1107      32      10      32     229      32     940      32

Cuts at:   2414    2583 
   Size:        169

  Fragments arranged by size: 

                1107     940     229     169      32      32      32      32
                  10
 
HincII GTy'rAC

Cuts at:      0    2401    2583 
   Size:       2401     182
 
KpnI G_GTAC'C

Cuts at:      0     433    2583 
   Size:        433    2150
 
MfeI C'AATT_G

Cuts at:      0    1952    2583 
   Size:       1952     631
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      44    2583 
   Size:         44    2539
 
MscI TGG'CCA

Cuts at:      0     625    2583 
   Size:        625    1958
 
MslI CAynn'nnrTG

Cuts at:      0    1366    2160    2461    2583 
   Size:       1366     794     301     122

  Fragments arranged by size: 

                1366     794     301     122
 
MspA1I CmG'CkG

Cuts at:      0    2055    2118    2583 
   Size:       2055      63     465

  Fragments arranged by size: 

                2055     465      63
 
NcoI C'CATG_G

Cuts at:      0    2456    2583 
   Size:       2456     127
 
NspI r_CATG'y

Cuts at:      0     622    1489    1590    2407    2583 
   Size:        622     867     101     817     176

  Fragments arranged by size: 

                 867     817     622     176     101
 
PasI CC'CwG_GG

Cuts at:      0    1606    2583 
   Size:       1606     977
 
PciI A'CATG_T

Cuts at:      0     618    2403    2583 
   Size:        618    1785     180

  Fragments arranged by size: 

                1785     618     180
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2253    2583 
   Size:       2253     330
 
PfoI T'CCnGG_A

Cuts at:      0     475     774    1318    2583 
   Size:        475     299     544    1265

  Fragments arranged by size: 

                1265     544     475     299
 
PpuMI rG'GwC_Cy

Cuts at:      0    2072    2583 
   Size:       2072     511
 
PspXI vC'TCGA_Gb

Cuts at:      0     251    2583 
   Size:        251    2332
 
PstI C_TGCA'G

Cuts at:      0    1655    2060    2179    2583 
   Size:       1655     405     119     404

  Fragments arranged by size: 

                1655     405     404     119
 
PvuII CAG'CTG

Cuts at:      0    2055    2118    2583 
   Size:       2055      63     465

  Fragments arranged by size: 

                2055     465      63
 
SacI G_AGCT'C

Cuts at:      0    1865    2583 
   Size:       1865     718
 
SfcI C'TryA_G

Cuts at:      0      90    1651    2056    2175    2583 
   Size:         90    1561     405     119     408

  Fragments arranged by size: 

                1561     408     405     119      90
 
SmlI C'TyrA_G

Cuts at:      0     205     251    1384    2080    2583 
   Size:        205      46    1133     696     503

  Fragments arranged by size: 

                1133     696     503     205      46
 
SspI AAT'ATT

Cuts at:      0     335    2583 
   Size:        335    2248
 
StuI AGG'CCT

Cuts at:      0    2095    2583 
   Size:       2095     488
 
StyI C'CwwG_G

Cuts at:      0     764    1294    2456    2583 
   Size:        764     530    1162     127

  Fragments arranged by size: 

                1162     764     530     127
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1320    2583 
   Size:       1320    1263
 
TatI w'GTAC_w

Cuts at:      0     255    1589    1898    2583 
   Size:        255    1334     309     685

  Fragments arranged by size: 

                1334     685     309     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     140     172     306     338     637     669    2464    2496 
   Size:        140      32     134      32     299      32    1795      32

Cuts at:   2496    2583 
   Size:         87

  Fragments arranged by size: 

                1795     299     140     134      87      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2012    2583 
   Size:       2012     571
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     705    2583 
   Size:        705    1878
 
XhoI C'TCGA_G

Cuts at:      0     251    2583 
   Size:        251    2332
 
XmnI GAAnn'nnTTC

Cuts at:      0     490    1146    2583 
   Size:        490     656    1437

  Fragments arranged by size: 

                1437     656     490
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     AlfI     AloI    ApaLI     ApoI
     AseI     AvaI     BaeI     BanI    BanII     BbsI     BcgI    BciVI
    BglII Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
   HincII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI    PspXI     PstI
    PvuII     SacI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
    AlwNI     ApaI     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI   Bpu10I    BsaAI
    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrFI
   BssHII    BssSI  BstZ17I     ClaI     DraI     DrdI     EagI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI     XbaI     XmaI     ZraI
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