Restriction map for pF1KB8490
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BalI        
BamHI       BbeI        BbrPI       BclI        BfrI        
BglI        BlnI        BsaAI       BsaI        BseAI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    EarI        EcoNI       EcoO65I     
EcoRV       FbaI        FseI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NarI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
ScaI        SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        SspI        StuI        SwaI        
Tru9I       XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        2395  GACGTC               GACGT^C
AccI         2398  GTCTAC               GT^[AC][GT]AC
AcyI         2395  GACGTC               G[GA]^CG[CT]C
AflIII        518  ACACGT               A^C[GA][CT]GT
Aor51HI      1976  AGCGCT               AGC^GCT
Asp700I       359  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1455  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         384  TGCGCA               TGC^GCA
BcgI          703  GCAGCAGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2543  AGATCT               A^GATCT
BsaBI         573  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2395  GACGTC               G[GA]^CG[CT]C
BsaWI        2051  ACCGGA               [AT]^CCGG[AT]
BsiEI         209  CGGCCG               CG[GA][CT]^CG
BsmBI        1453  GAGACG               CGTCTC(1/5)
Bsp1407I      140  TGTACA               T^GTACA
BsrGI         140  TGTACA               T^GTACA
BstXI        2024  CCAAACGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        2543  AGATCT               [GA]^GATC[CT]
DraIII       2376  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          209  CGGCCG               C^GGCCG
EclXI         209  CGGCCG               C^GGCCG
Eco47III     1976  AGCGCT               AGC^GCT
Eco52I        209  CGGCCG               C^GGCCG
EcoT22I      2323  ATGCAT               ATGCA^T
FspI          384  TGCGCA               TGC^GCA
Hin1I        2395  GACGTC               G[GA]^CG[CT]C
KspI          269  CCGCGG               CCGC^GG
MamI          573  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2543  AGATCT               [GA]^GATC[CT]
NcoI         1988  CCATGG               C^CATGG
NdeI         2326  CATATG               CA^TATG
NsiI         2323  ATGCAT               ATGCA^T
PflFI        1455  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1766  GACGCTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         269  CCGCGG               CCGC^GG
SbfI          826  CCTGCAGG             CCTGCA^GG
SexAI        2336  ACCTGGT              A^CC[AT]GGT
SgrAI        2284  CGCCGGCG             C[GA]^CCGG[CT]G
SphI         2561  GCATGC               GCATG^C
Sse8387I      826  CCTGCAGG             CCTGCA^GG
SspBI         140  TGTACA               T^GTACA
Tth111I      1455  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        2543  AGATCT               [GA]^GATC[CT]
XmnI          359  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         270  CGCG                 CG^CG
             1457  CGCG                
AcsI         2404  GAATTC               [GA]^AATT[CT]
             2446  GAATTC              
AocI          482  CCTCAGG              CC^T[ACGT]AGG
             1613  CCTCAGG             
ApoI         2404  GAATTC               [GA]^AATT[CT]
             2446  GAATTC              
AvaI          711  CTCGGG               C^[CT]CG[GA]G
             1309  CCCGAG              
BanI          832  GGTGCC               G^G[CT][GA]CC
             1428  GGCACC              
BbvCI         291  CCTCAGC              CCTCAGC(-5/-2)
             1593  CCTCAGC             
BciVI        2133  GGATAC               GTATCC(6/5)
              343  GTATCC              
BfaI           32  CTAG                 C^TAG
             1192  CTAG                
BsoBI         711  CTCGGG               C^[CT]CG[GA]G
             1309  CCCGAG              
BsrBI         212  CCGCTC               CCGCTC(-3/-3)
             2412  CCGCTC              
BsrDI         966  CATTGC               GCAATG(2/0)
              374  GCAATG              
BssSI        1524  CACGAG               CACGAG(-5/-1)
             1555  CACGAG              
BstUI         270  CGCG                 CG^CG
             1457  CGCG                
Bsu36I        482  CCTCAGG              CC^T[ACGT]AGG
             1613  CCTCAGG             
Eco81I        482  CCTCAGG              CC^T[ACGT]AGG
             1613  CCTCAGG             
EcoRI        2404  GAATTC               G^AATTC
             2446  GAATTC              
EcoT14I      1988  CCATGG               C^C[AT][AT]GG
             2129  CCAAGG              
MaeI           32  CTAG                 C^TAG
             1192  CTAG                
MvnI          270  CGCG                 CG^CG
             1457  CGCG                
NaeI         1645  GCCGGC               GCC^GGC
             2285  GCCGGC              
NgoMIV       1645  GCCGGC               G^CCGGC
             2285  GCCGGC              
PflMI         309  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2375  CCACCAAGTGG         
PpuMI        2303  GGGACCT              [GA]G^G[AT]CC[CT]
             2333  GGGACCT             
PvuII         985  CAGCTG               CAG^CTG
             1355  CAGCTG              
StyI         1988  CCATGG               C^C[AT][AT]GG
             2129  CCAAGG              
TfiI         1788  GAATC                G^A[AT]TC
             1891  GAATC               
Van91I        309  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2375  CCACCAAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaII       BanII       BbsI        
BbvI        BcnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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