Restriction map for pF1KB8490
 (Linear) (Six-base) MAPSORT of: KIBB8490.seq  Check: 1155  from: 1  to: 2577



KIBB8490.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     162     820    1037    2577 
   Size:        162     658     217    1540

  Fragments arranged by size: 

                1540     658     217     162
 
AatII G_ACGT'C

Cuts at:      0    2399    2577 
   Size:       2399     178
 
AccI GT'mk_AC

Cuts at:      0    2399    2577 
   Size:       2399     178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1337    2560    2577 
   Size:        350     987    1223      17

  Fragments arranged by size: 

                1223     987     350      17
 
AfeI AGC'GCT

Cuts at:      0    1978    2577 
   Size:       1978     599
 
AflIII A'CryG_T

Cuts at:      0     518    2577 
   Size:        518    2059
 
AleI CACnn'nnGTG

Cuts at:      0    1221    2577 
   Size:       1221    1356
 
AlwNI CAG_nnn'CTG

Cuts at:      0      41      83     189     222     315     783    2077    2158 
   Size:         41      42     106      33      93     468    1294      81

Cuts at:   2158    2577 
   Size:        419

  Fragments arranged by size: 

                1294     468     419     106      93      81      42      41
                  33
 
ApaI G_GGCC'C

Cuts at:      0     128     459     718    2534    2577 
   Size:        128     331     259    1816      43

  Fragments arranged by size: 

                1816     331     259     128      43
 
ApoI r'AATT_y

Cuts at:      0    2404    2446    2577 
   Size:       2404      42     131

  Fragments arranged by size: 

                2404     131      42
 
AvaI C'yCGr_G

Cuts at:      0     711    1309    2577 
   Size:        711     598    1268

  Fragments arranged by size: 

                1268     711     598
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     541     574    2577 
   Size:        541      33    2003

  Fragments arranged by size: 

                2003     541      33
 
BanI G'GyrC_C

Cuts at:      0     832    1428    2577 
   Size:        832     596    1149

  Fragments arranged by size: 

                1149     832     596
 
BanII G_rGCy'C

Cuts at:      0     128     459     623     718     929    1121    1531    1880 
   Size:        128     331     164      95     211     192     410     349

Cuts at:   1880    1934    2315    2375    2534    2577 
   Size:         54     381      60     159      43

  Fragments arranged by size: 

                 410     381     349     331     211     192     164     159
                 128      95      60      54      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     515    2213    2399    2577 
   Size:        515    1698     186     178

  Fragments arranged by size: 

                1698     515     186     178
 
BbvCI CC'TCA_GC

Cuts at:      0     292    1594    2577 
   Size:        292    1302     983

  Fragments arranged by size: 

                1302     983     292
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     692     726    2577 
   Size:        692      34    1851

  Fragments arranged by size: 

                1851     692      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     354    2127    2577 
   Size:        354    1773     450

  Fragments arranged by size: 

                1773     450     354
 
BfrBI ATG'CAT

Cuts at:      0    2325    2577 
   Size:       2325     252
 
BglII A'GATC_T

Cuts at:      0    2543    2577 
   Size:       2543      34
 
BlpI GC'TnA_GC

Cuts at:      0     494     928     983    1115    2577 
   Size:        494     434      55     132    1462

  Fragments arranged by size: 

                1462     494     434     132      55
 
Bme1580I G_kGCm'C

Cuts at:      0     128     459     570     718    1322    1394    2165    2457 
   Size:        128     331     111     148     604      72     771     292

Cuts at:   2457    2534    2577 
   Size:         77      43

  Fragments arranged by size: 

                 771     604     331     292     148     128     111      77
                  72      43
 
BmgBI CAC'GTC

Cuts at:      0     521    2577 
   Size:        521    2056
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     393    1298    2577 
   Size:        393     905    1279

  Fragments arranged by size: 

                1279     905     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     292    1144    1204    1620    2196    2329    2577 
   Size:        262      30     852      60     416     576     133     248

  Fragments arranged by size: 

                 852     576     416     262     248     133      60      30
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     292     617     815    1594    2577 
   Size:        292     325     198     779     983

  Fragments arranged by size: 

                 983     779     325     292     198
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1777    1906    2217    2473    2551    2577 
   Size:       1777     129     311     256      78      26

  Fragments arranged by size: 

                1777     311     256     129      78      26
 
BsaBI GATnn'nnATC

Cuts at:      0     577    2577 
   Size:        577    2000
 
BsaHI Gr'CG_yC

Cuts at:      0    2396    2577 
   Size:       2396     181
 
BsaWI w'CCGG_w

Cuts at:      0    2051    2577 
   Size:       2051     526
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     986    1016    1618    1648    2577 
   Size:        986      30     602      30     929

  Fragments arranged by size: 

                 986     929     602      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     436     994    1888    1915    2577 
   Size:        436     558     894      27     662

  Fragments arranged by size: 

                 894     662     558     436      27
 
BseYI C'CCAG_C

Cuts at:      0     202     242     302     429     440     528     742     792 
   Size:        202      40      60     127      11      88     214      50

Cuts at:    792     922    1088    1127    1165    1394    1548    2526    2577 
   Size:        130     166      39      38     229     154     978      51

  Fragments arranged by size: 

                 978     229     214     202     166     154     130     127
                  88      60      51      50      40      39      38      11
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     190     631    1225    2577 
   Size:        175      15     441     594    1352

  Fragments arranged by size: 

                1352     594     441     175      15
 
BsiEI CG_ry'CG

Cuts at:      0     212    2577 
   Size:        212    2365
 
BsiHKAI G_wGCw'C

Cuts at:      0     509     623    1109    1911    1934    2315    2577 
   Size:        509     114     486     802      23     381     262

  Fragments arranged by size: 

                 802     509     486     381     262     114      23
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1447    2577 
   Size:       1447    1130
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     459     509     570     623     718     929    1109 
   Size:        128     331      50      61      53      95     211     180

Cuts at:   1109    1121    1322    1394    1531    1880    1911    1934    2165 
   Size:         12     201      72     137     349      31      23     231

Cuts at:   2165    2315    2375    2457    2534    2577 
   Size:        150      60      82      77      43

  Fragments arranged by size: 

                 349     331     231     211     201     180     150     137
                 128      95      82      77      72      61      60      53
                  50      43      31      23      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     162     230     820    1037    1247    1328    2577 
   Size:        162      68     590     217     210      81    1249

  Fragments arranged by size: 

                1249     590     217     210     162      81      68
 
BsrBI CCG'CTC

Cuts at:      0     214    2414    2577 
   Size:        214    2200     163

  Fragments arranged by size: 

                2200     214     163
 
BsrDI GCAATG_nn'

Cuts at:      0     381     965    2577 
   Size:        381     584    1612

  Fragments arranged by size: 

                1612     584     381
 
BsrFI r'CCGG_y

Cuts at:      0     158    1645    2285    2577 
   Size:        158    1487     640     292

  Fragments arranged by size: 

                1487     640     292     158
 
BsrGI T'GTAC_A

Cuts at:      0     140    2577 
   Size:        140    2437
 
BssSI C'ACGA_G

Cuts at:      0    1524    1555    2577 
   Size:       1524      31    1022

  Fragments arranged by size: 

                1524    1022      31
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      83     168     189     258     504    2077    2478    2577 
   Size:         83      85      21      69     246    1573     401      99

  Fragments arranged by size: 

                1573     401     246      99      85      83      69      21
            
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2031    2577 
   Size:       2031     546
 
BstYI r'GATC_y

Cuts at:      0    2543    2577 
   Size:       2543      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     483    1614    2577 
   Size:        483    1131     963

  Fragments arranged by size: 

                1131     963     483
 
BtgI C'CryG_G

Cuts at:      0     269     590    1988    2577 
   Size:        269     321    1398     589

  Fragments arranged by size: 

                1398     589     321     269
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     474    1626    2577 
   Size:        474    1152     951

  Fragments arranged by size: 

                1152     951     474
 
BtsI GCAGTG_nn'

Cuts at:      0      42      84     105     900    1229    1270    1405    1493 
   Size:         42      42      21     795     329      41     135      88

Cuts at:   1493    1649    2577 
   Size:        156     928

  Fragments arranged by size: 

                 928     795     329     156     135      88      42      42
                  41      21
 
DraIII CAC_nnn'GTG

Cuts at:      0    2381    2577 
   Size:       2381     196
 
EaeI y'GGCC_r

Cuts at:      0     111     209     593    1938    2577 
   Size:        111      98     384    1345     639

  Fragments arranged by size: 

                1345     639     384     111      98
 
EagI C'GGCC_G

Cuts at:      0     209    2577 
   Size:        209    2368
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1762    2577 
   Size:       1762     815
 
EcoICRI GAG'CTC

Cuts at:      0     621    1932    2313    2577 
   Size:        621    1311     381     264

  Fragments arranged by size: 

                1311     621     381     264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     292     350    1144    1204    1337    1620    2196 
   Size:        262      30      58     794      60     133     283     576

Cuts at:   2196    2329    2560    2577 
   Size:        133     231      17

  Fragments arranged by size: 

                 794     576     283     262     231     133     133      60
                  58      30      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125     186     390     715    2304    2334    2530    2577 
   Size:        125      61     204     325    1589      30     196      47

  Fragments arranged by size: 

                1589     325     204     196     125      61      47      30
            
 
EcoRI G'AATT_C

Cuts at:      0    2404    2446    2577 
   Size:       2404      42     131

  Fragments arranged by size: 

                2404     131      42
 
FspI TGC'GCA

Cuts at:      0     386    2577 
   Size:        386    2191
 
HaeII r_GCGC'y

Cuts at:      0     277     302    1980    2513    2577 
   Size:        277      25    1678     533      64

  Fragments arranged by size: 

                1678     533     277      64      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1444    1476    2577 
   Size:       1444      32    1101

  Fragments arranged by size: 

                1444    1101      32
 
MslI CAynn'nnrTG

Cuts at:      0    1221    1498    2326    2560    2577 
   Size:       1221     277     828     234      17

  Fragments arranged by size: 

                1221     828     277     234      17
 
MspA1I CmG'CkG

Cuts at:      0      62     271     700     987    1357    2301    2577 
   Size:         62     209     429     287     370     944     276

  Fragments arranged by size: 

                 944     429     370     287     276     209      62
 
NaeI GCC'GGC

Cuts at:      0    1647    2287    2577 
   Size:       1647     640     290

  Fragments arranged by size: 

                1647     640     290
 
NcoI C'CATG_G

Cuts at:      0    1988    2577 
   Size:       1988     589
 
NdeI CA'TA_TG

Cuts at:      0    2327    2577 
   Size:       2327     250
 
NgoMIV G'CCGG_C

Cuts at:      0    1645    2285    2577 
   Size:       1645     640     292

  Fragments arranged by size: 

                1645     640     292
 
NsiI A_TGCA'T

Cuts at:      0    2327    2577 
   Size:       2327     250
 
NspI r_CATG'y

Cuts at:      0    1717    2325    2565    2577 
   Size:       1717     608     240      12

  Fragments arranged by size: 

                1717     608     240      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315    2381    2577 
   Size:        315    2066     196

  Fragments arranged by size: 

                2066     315     196
 
PfoI T'CCnGG_A

Cuts at:      0    1736    2577 
   Size:       1736     841
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1612    1644    2577 
   Size:       1612      32     933

  Fragments arranged by size: 

                1612     933      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2304    2334    2577 
   Size:       2304      30     243

  Fragments arranged by size: 

                2304     243      30
 
PshAI GACnn'nnGTC

Cuts at:      0    1770    2577 
   Size:       1770     807
 
PspOMI G'GGCC_C

Cuts at:      0     124     455     714    2530    2577 
   Size:        124     331     259    1816      47

  Fragments arranged by size: 

                1816     331     259     124      47
 
PstI C_TGCA'G

Cuts at:      0     227     266     477     831     992    1034    1376    2066 
   Size:        227      39     211     354     161      42     342     690

Cuts at:   2066    2577 
   Size:        511

  Fragments arranged by size: 

                 690     511     354     342     227     211     161      42
                  39
 
PvuII CAG'CTG

Cuts at:      0     987    1357    2577 
   Size:        987     370    1220

  Fragments arranged by size: 

                1220     987     370
 
SacI G_AGCT'C

Cuts at:      0     623    1934    2315    2577 
   Size:        623    1311     381     262

  Fragments arranged by size: 

                1311     623     381     262
 
SacII CC_GC'GG

Cuts at:      0     272    2577 
   Size:        272    2305
 
SbfI CC_TGCA'GG

Cuts at:      0     831    2577 
   Size:        831    1746
 
SexAI A'CCwGG_T

Cuts at:      0    2336    2577 
   Size:       2336     241
 
SfcI C'TryA_G

Cuts at:      0     223     262     473     827     988    1030    1372    2062 
   Size:        223      39     211     354     161      42     342     690

Cuts at:   2062    2577 
   Size:        515

  Fragments arranged by size: 

                 690     515     354     342     223     211     161      42
                  39
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2285    2577 
   Size:       2285     292
 
SmlI C'TyrA_G

Cuts at:      0    1792    1921    2232    2488    2566    2577 
   Size:       1792     129     311     256      78      11

  Fragments arranged by size: 

                1792     311     256     129      78      11
 
SphI G_CATG'C

Cuts at:      0    2565    2577 
   Size:       2565      12
 
StyI C'CwwG_G

Cuts at:      0    1988    2129    2577 
   Size:       1988     141     448

  Fragments arranged by size: 

                1988     448     141
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1603    2271    2577 
   Size:       1603     668     306

  Fragments arranged by size: 

                1603     668     306
 
TatI w'GTAC_w

Cuts at:      0     140    2577 
   Size:        140    2437
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     986    1018    1799    1831    2577 
   Size:        986      32     781      32     746

  Fragments arranged by size: 

                 986     781     746      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1458    2577 
   Size:       1458    1119
 
XmnI GAAnn'nnTTC

Cuts at:      0     363    2577 
   Size:        363    2214
 
ZraI GAC'GTC

Cuts at:      0    2397    2577 
   Size:       2397     180
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AleI    AlwNI
     ApaI     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NsiI     NspI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII
     SbfI    SexAI     SfcI    SgrAI     SmlI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BclI     BglI
     BmtI     BplI     BsaI    BsaAI    BsiWI     BsmI    BspEI    BspHI
   BssHII    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI     DrdI
     EarI    EcoNI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI
     ScaI     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     XbaI     XcmI     XhoI     XmaI
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