Restriction map for
pF1KB5897
(Circular) (Six-base) MAPSORT of: pF1KB5897.seq Check: 13 from: 1 to: 5624
pF1KB5897 5624 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 933 933
Size: 5624
AccI GT'mk_AC
Cuts at: 2174 2662 2174
Size: 488 5136
Acc65I G'GTAC_C
Cuts at: 2640 2640
Size: 5624
AclI AA'CG_TT
Cuts at: 5464 5464
Size: 5624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 197 1274 1311 1921 2308 2452 3449 3881
Size: 49 1077 37 610 387 144 997 432
Cuts at: 3881 4628 4896 148
Size: 747 268 876
Fragments arranged by size:
1077 997 876 747 610 432 387 268
144 49 37
AflIII A'CryG_T
Cuts at: 2976 4080 5057 2976
Size: 1104 977 3543
Fragments arranged by size:
3543 1104 977
AgeI A'CCGG_T
Cuts at: 4012 4012
Size: 5624
AleI CACnn'nnGTG
Cuts at: 2351 2351
Size: 5624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 529 542 529
Size: 13 5611
AlwNI CAG_nnn'CTG
Cuts at: 178 250 286 1107 2357 2929 4496 178
Size: 72 36 821 1250 572 1567 1306
Fragments arranged by size:
1567 1306 1250 821 572 72 36
ApaI G_GGCC'C
Cuts at: 296 296
Size: 5624
ApaLI G'TGCA_C
Cuts at: 4394 4394
Size: 5624
ApoI r'AATT_y
Cuts at: 1180 2628 4977 5233 1180
Size: 1448 2349 256 1571
Fragments arranged by size:
2349 1571 1448 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5624
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
AvaI C'yCGr_G
Cuts at: 295 851 1144 1562 2178 2644 295
Size: 556 293 418 616 466 3275
Fragments arranged by size:
3275 616 556 466 418 293
BamHI G'GATC_C
Cuts at: 330 2649 330
Size: 2319 3305
BanI G'GyrC_C
Cuts at: 240 1140 2004 2640 3054 3302 3337 240
Size: 900 864 636 414 248 35 2527
Fragments arranged by size:
2527 900 864 636 414 248 35
BanII G_rGCy'C
Cuts at: 296 878 2186 2638 3668 296
Size: 582 1308 452 1030 2252
Fragments arranged by size:
2252 1308 1030 582 452
BbeI G_GCGC'C
Cuts at: 3058 3306 3058
Size: 248 5376
BbsI GAAGACnn'nnnn_
Cuts at: 423 1830 2440 423
Size: 1407 610 3607
Fragments arranged by size:
3607 1407 610
BbvCI CC'TCA_GC
Cuts at: 953 953
Size: 5624
BciVI GTATCCnnnnn_n'
Cuts at: 591 3522 4283 5624 591
Size: 2931 761 1341 591
Fragments arranged by size:
2931 1341 761 591
BclI T'GATC_A
Cuts at: 1766 3144 1766
Size: 1378 4246
BfrBI ATG'CAT
Cuts at: 731 731
Size: 5624
BglI GCCn_nnn'nGGC
Cuts at: 1558 1558
Size: 5624
BglII A'GATC_T
Cuts at: 1205 3139 5176 1205
Size: 1934 2037 1653
Fragments arranged by size:
2037 1934 1653
BlpI GC'TnA_GC
Cuts at: 2185 2736 2185
Size: 551 5073
Bme1580I G_kGCm'C
Cuts at: 296 1013 1145 2009 3249 3342 4398 296
Size: 717 132 864 1240 93 1056 1522
Fragments arranged by size:
1522 1240 1056 864 717 132 93
BmgBI CAC'GTC
Cuts at: 960 960
Size: 5624
BmrI ACTGGGnnnn_n'
Cuts at: 296 924 2345 2836 2917 3252 5396 296
Size: 628 1421 491 81 335 2144 524
Fragments arranged by size:
2144 1421 628 524 491 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3991 3991
Size: 5624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1453 1603 2590 1216
Size: 237 150 987 4250
Fragments arranged by size:
4250 987 237 150
Bpu10I CC'TnA_GC
Cuts at: 349 953 349
Size: 604 5020
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 937 2578 2800 3679 4171 4469 436
Size: 501 1641 222 879 492 298 1591
Fragments arranged by size:
1641 1591 879 501 492 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 950 5328 950
Size: 4378 1246
BsaAI yAC'GTr
Cuts at: 579 1008 2469 2977 579
Size: 429 1461 508 3226
Fragments arranged by size:
3226 1461 508 429
BsaBI GATnn'nnATC
Cuts at: 6 3138 5175 6
Size: 3132 2037 455
Fragments arranged by size:
3132 2037 455
BsaHI Gr'CG_yC
Cuts at: 1633 3055 3303 4005 5501 1633
Size: 1422 248 702 1496 1756
Fragments arranged by size:
1756 1496 1422 702 248
BsaWI w'CCGG_w
Cuts at: 1073 2813 3334 4012 4286 4433 4844 1073
Size: 1740 521 678 274 147 411 1853
Fragments arranged by size:
1853 1740 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 177 207 583 613 177
Size: 30 376 30 5188
Fragments arranged by size:
5188 376 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1213 1216 1600 1213
Size: 3 384 5237
Fragments arranged by size:
5237 384 3
BseYI C'CCAG_C
Cuts at: 171 202 255 267 624 1312 1333 1524 3049
Size: 31 53 12 357 688 21 191 1525
Cuts at: 3049 3807 4384 4880 171
Size: 758 577 496 915
Fragments arranged by size:
1525 915 758 688 577 496 357 191
53 31 21 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 482 844 1319 182
Size: 300 362 475 4487
Fragments arranged by size:
4487 475 362 300
BsiEI CG_ry'CG
Cuts at: 104 3166 3212 4420 104
Size: 3062 46 1208 1308
Fragments arranged by size:
3062 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 563 878 2638 3416 3606 4398 563
Size: 315 1760 778 190 792 1789
Fragments arranged by size:
1789 1760 792 778 315 190
BsmI GAATG_Cn'
Cuts at: 2237 2237
Size: 5624
BsmBI CGTCTCn'nnnn_
Cuts at: 1528 1528
Size: 5624
Bsp1286I G_dGCh'C
Cuts at: 296 563 878 1013 1145 2009 2186 2638 3249
Size: 267 315 135 132 864 177 452 611
Cuts at: 3249 3342 3416 3606 3668 4398 296
Size: 93 74 190 62 730 1522
Fragments arranged by size:
1522 864 730 611 452 315 267 190
177 135 132 93 74 62
BspEI T'CCGG_A
Cuts at: 4844 4844
Size: 5624
BspHI T'CATG_A
Cuts at: 4800 4800
Size: 5624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 684 933 1854 2333 2504 2674 2888 3190 3571
Size: 249 921 479 171 170 214 302 381
Cuts at: 3571 684
Size: 2737
Fragments arranged by size:
2737 921 479 381 302 249 214 171
170
BsrBI CCG'CTC
Cuts at: 50 323 858 2448 3916 3970 5454 5619 50
Size: 273 535 1590 1468 54 1484 165 55
Fragments arranged by size:
1590 1484 1468 535 273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3536 3536
Size: 5624
BsrFI r'CCGG_y
Cuts at: 271 1097 1646 3622 3803 4012 271
Size: 826 549 1976 181 209 1883
Fragments arranged by size:
1976 1883 826 549 209 181
BssHII G'CGCG_C
Cuts at: 363 402 363
Size: 39 5585
BssSI C'ACGA_G
Cuts at: 1666 3895 4253 1666
Size: 2229 358 3037
Fragments arranged by size:
3037 2229 358
BstAPI GCAn_nnn'nTGC
Cuts at: 810 3001 810
Size: 2191 3433
BstBI TT'CG_AA
Cuts at: 3985 3985
Size: 5624
BstXI CCAn_nnnn'nTGG
Cuts at: 1479 2568 1479
Size: 1089 4535
BstYI r'GATC_y
Cuts at: 1 330 510 1205 2162 2213 2649 3116 3139
Size: 329 180 695 957 51 436 467 23
Cuts at: 3139 3474 3720 4732 4818 4830 5176 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 957 695 467 449 436 346 335
329 246 180 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 1712 1712
Size: 5624
BtgI C'CryG_G
Cuts at: 107 1472 1593 107
Size: 1365 121 4138
Fragments arranged by size:
4138 1365 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2493 3574 3755 3918 2493
Size: 1081 181 163 4199
Fragments arranged by size:
4199 1081 181 163
BtsI GCAGTG_nn'
Cuts at: 494 2867 494
Size: 2373 3251
DraI TTT'AAA
Cuts at: 2623 2623
Size: 5624
DrdI GACnn_nn'nnGTC
Cuts at: 3330 4188 3330
Size: 858 4766
EaeI y'GGCC_r
Cuts at: 1282 1550 2489 3209 3383 3774 3801 5146 1282
Size: 268 939 720 174 391 27 1345 1760
Fragments arranged by size:
1760 1345 939 720 391 268 174 27
EagI C'GGCC_G
Cuts at: 3209 3209
Size: 5624
EarI CTCTTCn'nnn_
Cuts at: 658 668 715 1144 1399 3647 3857 658
Size: 10 47 429 255 2248 210 2425
Fragments arranged by size:
2425 2248 429 255 210 47 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3832 4142 4288 5434 127
Size: 3705 310 146 1146 317
Fragments arranged by size:
3705 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 876 2636 876
Size: 1760 3864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 197 1216 1274 1311 1453 1603 1921 2308
Size: 49 1019 58 37 142 150 318 387
Cuts at: 2308 2452 2590 3449 3881 4628 4896 148
Size: 144 138 859 432 747 268 876
Fragments arranged by size:
1019 876 859 747 432 387 318 268
150 144 142 138 58 49 37
EcoNI CCTnn'n_nnAGG
Cuts at: 1394 1394
Size: 5624
EcoO109I rG'GnC_Cy
Cuts at: 126 292 764 1154 1528 1880 2292 2391 2763
Size: 166 472 390 374 352 412 99 372
Cuts at: 2763 126
Size: 2987
Fragments arranged by size:
2987 472 412 390 374 372 352 166
99
EcoRI G'AATT_C
Cuts at: 2628 2628
Size: 5624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 906 3643 906
Size: 2737 2887
FspI TGC'GCA
Cuts at: 149 3405 149
Size: 3256 2368
HaeII r_GCGC'y
Cuts at: 1593 2567 3058 3306 4328 5307 1593
Size: 974 491 248 1022 979 1910
Fragments arranged by size:
1910 1022 979 974 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 565 583 597 615 3466 3496 3498
Size: 32 320 18 14 18 2851 30 2
Cuts at: 3498 3528 213
Size: 30 2309
Fragments arranged by size:
2851 2309 320 32 30 30 18 18
14 2
HincII GTy'rAC
Cuts at: 2663 2663
Size: 5624
HindIII A'AGCT_T
Cuts at: 412 913 412
Size: 501 5123
KasI G'GCGC_C
Cuts at: 3054 3302 3054
Size: 248 5376
KpnI G_GTAC'C
Cuts at: 2644 2644
Size: 5624
MluI A'CGCG_T
Cuts at: 5057 5057
Size: 5624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 214 250 2366 4295 4479 214
Size: 36 2116 1929 184 1359
Fragments arranged by size:
2116 1929 1359 184 36
MscI TGG'CCA
Cuts at: 3385 3385
Size: 5624
MslI CAynn'nnrTG
Cuts at: 1471 2002 2351 3613 3740 1471
Size: 531 349 1262 127 3355
Fragments arranged by size:
3355 1262 531 349 127
MspA1I CmG'CkG
Cuts at: 259 526 1104 1272 1303 1595 1698 2189 2502
Size: 267 578 168 31 292 103 491 313
Cuts at: 2502 2555 2735 3049 3409 4422 4667 259
Size: 53 180 314 360 1013 245 1216
Fragments arranged by size:
1216 1013 578 491 360 314 313 292
267 245 180 168 103 53 31
NaeI GCC'GGC
Cuts at: 273 1099 3805 273
Size: 826 2706 2092
Fragments arranged by size:
2706 2092 826
NarI GG'CG_CC
Cuts at: 3055 3303 3055
Size: 248 5376
NcoI C'CATG_G
Cuts at: 107 1472 107
Size: 1365 4259
NgoMIV G'CCGG_C
Cuts at: 271 1097 3803 271
Size: 826 2706 2092
Fragments arranged by size:
2706 2092 826
NruI TCG'CGA
Cuts at: 2522 2522
Size: 5624
NsiI A_TGCA'T
Cuts at: 733 733
Size: 5624
NspI r_CATG'y
Cuts at: 1710 1809 2677 3177 4084 1710
Size: 99 868 500 907 3250
Fragments arranged by size:
3250 907 868 500 99
PasI CC'CwG_GG
Cuts at: 768 1818 768
Size: 1050 4574
PciI A'CATG_T
Cuts at: 4080 4080
Size: 5624
PflMI CCAn_nnn'nTGG
Cuts at: 2357 2357
Size: 5624
PmeI GTTT'AAAC
Cuts at: 2623 2623
Size: 5624
PmlI CAC'GTG
Cuts at: 1008 1008
Size: 5624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 5592
PpuMI rG'GwC_Cy
Cuts at: 764 1154 1880 2292 764
Size: 390 726 412 4096
Fragments arranged by size:
4096 726 412 390
PspOMI G'GGCC_C
Cuts at: 292 292
Size: 5624
PstI C_TGCA'G
Cuts at: 264 518 740 1380 1679 2501 2671 3356 264
Size: 254 222 640 299 822 170 685 2532
Fragments arranged by size:
2532 822 685 640 299 254 222 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5624
PvuII CAG'CTG
Cuts at: 259 526 1104 1272 1698 2189 3049 3409 259
Size: 267 578 168 426 491 860 360 2474
Fragments arranged by size:
2474 860 578 491 426 360 267 168
RsrII CG'GwC_CG
Cuts at: 3819 3819
Size: 5624
SacI G_AGCT'C
Cuts at: 878 2638 878
Size: 1760 3864
SacII CC_GC'GG
Cuts at: 1596 1596
Size: 5624
SalI G'TCGA_C
Cuts at: 2661 2661
Size: 5624
SanDI GG'GwC_CC
Cuts at: 764 1880 764
Size: 1116 4508
SapI GCTCTTCn'nnn_
Cuts at: 3647 3857 3647
Size: 210 5414
SbfI CC_TGCA'GG
Cuts at: 2671 2671
Size: 5624
ScaI AGT'ACT
Cuts at: 2548 5141 2548
Size: 2593 3031
SfcI C'TryA_G
Cuts at: 33 260 514 736 1376 1675 2497 2667 3352
Size: 227 254 222 640 299 822 170 685
Cuts at: 3352 4345 4536 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 822 685 640 299 254 227
222 191 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 1558 1558
Size: 5624
SfoI GGC'GCC
Cuts at: 3056 3304 3056
Size: 248 5376
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
SmaI CCC'GGG
Cuts at: 297 853 1564 2180 2646 297
Size: 556 711 616 466 3275
Fragments arranged by size:
3275 711 616 556 466
SmlI C'TyrA_G
Cuts at: 415 916 2593 2779 3694 4186 4448 415
Size: 501 1677 186 915 492 262 1591
Fragments arranged by size:
1677 1591 915 501 492 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5624
SphI G_CATG'C
Cuts at: 1710 2677 3177 1710
Size: 967 500 4157
Fragments arranged by size:
4157 967 500
SspI AAT'ATT
Cuts at: 798 798
Size: 5624
StuI AGG'CCT
Cuts at: 2606 2606
Size: 5624
StyI C'CwwG_G
Cuts at: 107 566 1472 1637 1991 2758 3112 107
Size: 459 906 165 354 767 354 2619
Fragments arranged by size:
2619 906 767 459 354 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 216 1330 1450 1831 3207 3340 3799 4008 216
Size: 1114 120 381 1376 133 459 209 1832
Fragments arranged by size:
1832 1376 1114 459 381 209 133 120
TatI w'GTAC_w
Cuts at: 827 2525 2546 5139 827
Size: 1698 21 2593 1312
Fragments arranged by size:
2593 1698 1312 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5141 5173 5141
Size: 32 5592
Tth111I GACn'n_nGTC
Cuts at: 3421 3421
Size: 5624
XbaI T'CTAG_A
Cuts at: 2655 2655
Size: 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3119 3119
Size: 5624
XmaI C'CCGG_G
Cuts at: 295 851 1562 2178 2644 295
Size: 556 711 616 466 3275
Fragments arranged by size:
3275 711 616 556 466
XmnI GAAnn'nnTTC
Cuts at: 652 2578 652
Size: 1926 3698
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BmtI BsiWI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI PacI PfoI PshAI PsiI PspXI PsrI SexAI
SgrAI SnaBI SrfI SwaI XhoI ZraI