Restriction map for pF1KB5764
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MaeII       
MfeI        MluI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SphI        Sse8387I    SspBI       SwaI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI         2082  GTCGAC               GT^[AC][GT]AC
AccII          57  CGCG                 CG^CG
AccIII       2057  TCCGGA               T^CCGGA
AcsI          524  AAATTT               [GA]^AATT[CT]
Alw44I        919  GTGCAC               G^TGCAC
ApaLI         919  GTGCAC               G^TGCAC
ApoI          524  AAATTT               [GA]^AATT[CT]
AseI         1537  ATTAAT               AT^TAAT
AsnI         1537  ATTAAT               AT^TAAT
AspHI         919  GTGCAC               G[AT]GC[AT]^C
AspI         1098  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1163  CCCGGG               C^[CT]CG[GA]G
BalI          311  TGGCCA               TGG^CCA
BanI          193  GGCACC               G^G[CT][GA]CC
BanII        1005  GGGCTC               G[GA]GC[CT]^C
BbsI         1104  GTCTTC               GAAGAC(2/6)
BciVI        1347  GGATAC               GTATCC(6/5)
BglII         213  AGATCT               A^GATCT
BpuAI        1104  GTCTTC               GAAGAC(2/6)
BsaBI         174  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2057  TCCGGA               [AT]^CCGG[AT]
BseAI        2057  TCCGGA               T^CCGGA
BsiHKAI       919  GTGCAC               G[AT]GC[AT]^C
BsmI         2035  GAATGC               GAATGC(1/-1)
BsoBI        1163  CCCGGG               C^[CT]CG[GA]G
BspEI        2057  TCCGGA               T^CCGGA
BstUI          57  CGCG                 CG^CG
DraI          495  TTTAAA               TTT^AAA
DsaI          635  CCGTGG               C^C[GA][CT]GG
EcoNI         948  CCTGCACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1616  CCTTGG               C^C[AT][AT]GG
EcoT22I      1181  ATGCAT               ATGCA^T
HincII       2082  GTCGAC               GT[CT]^[GA]AC
HindII       2082  GTCGAC               GT[CT]^[GA]AC
MamI          174  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         311  TGGCCA               TGG^CCA
MroI         2057  TCCGGA               T^CCGGA
MscI          311  TGGCCA               TGG^CCA
MslI         1983  CACTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           57  CGCG                 CG^CG
NsiI         1181  ATGCAT               ATGCA^T
PflFI        1098  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1537  ATTAAT               AT^TAAT
PvuII         356  CAGCTG               CAG^CTG
SalI         2082  GTCGAC               G^TCGAC
SmaI         1163  CCCGGG               CCC^GGG
SmlI          813  CTTGAG               C^T[CT][GA]AG
SspI          643  AATATT               AAT^ATT
StuI          162  AGGCCT               AGG^CCT
StyI         1616  CCTTGG               C^C[AT][AT]GG
Tsp45I       1234  GTCAC                ^GT[GC]AC
Tth111I      1098  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1777  CCAGAGAGTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1163  CCCGGG               C^CCGGG
XmaI         1163  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BsgI          949  CTGCAC               GTGCAG(16/14)
              976  GTGCAG              
BspMI        2234  ACCTGC               ACCTGC(4/8)
              542  GCAGGT              
BsrBI        1741  CCGCTC               CCGCTC(-3/-3)
              970  GAGCGG              
BsrI          275  CCAGT                ACTGG(1/-1)
             1214  CCAGT               
BssSI         480  CACGAG               CACGAG(-5/-1)
              952  CACGAG              
BstXI         183  CCAAGACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2125  CCAAACTCCTGG        
BstYI         179  AGATCC               [GA]^GATC[CT]
              213  AGATCT              
CfoI         2039  GCGC                 GCG^C
             2172  GCGC                
EaeI           79  CGGCCA               [CT]^GGCC[GA]
              311  TGGCCA              
EarI          778  CTCTTC               CTCTTC(1/4)
             1380  GAAGAG              
FauI           61  GCGGG                CCCGC(4/6)
              972  GCGGG               
HhaI         2039  GCGC                 GCG^C
             2172  GCGC                
HinP1I       2039  GCGC                 G^CGC
             2172  GCGC                
Ksp632I       778  CTCTTC               CTCTTC(1/4)
             1380  GAAGAG              
MflI          179  AGATCC               [GA]^GATC[CT]
              213  AGATCT              
MspA1I         76  CAGCGG               C[AC]G^C[GT]G
              356  CAGCTG              
PleI         1521  GAGTC                GAGTC(4/5)
             2080  GAGTC               
SpeI          244  ACTAGT               A^CTAGT
             1510  ACTAGT              
XhoII         179  AGATCC               [GA]^GATC[CT]
              213  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     Van91I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com