Restriction map for pF1KB5764
 (Linear) (Six-base) MAPSORT of: KIBB5764.seq  Check: 1931  from: 1  to: 2268



KIBB5764.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:      0    2083    2268 
   Size:       2083     185
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1334    1349    1864    2268 
   Size:        187    1147      15     515     404

  Fragments arranged by size: 

                1147     515     404     187      15
 
AleI CACnn'nnGTG

Cuts at:      0    1987    2268 
   Size:       1987     281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1541    1573    2268 
   Size:       1541      32     695

  Fragments arranged by size: 

                1541     695      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     604    1197    1309    2268 
   Size:        604     593     112     959

  Fragments arranged by size: 

                 959     604     593     112
 
ApaLI G'TGCA_C

Cuts at:      0     919    2268 
   Size:        919    1349
 
ApoI r'AATT_y

Cuts at:      0     524    2268 
   Size:        524    1744
 
AseI AT'TA_AT

Cuts at:      0    1538    2268 
   Size:       1538     730
 
AvaI C'yCGr_G

Cuts at:      0    1163    2268 
   Size:       1163    1105
 
BanI G'GyrC_C

Cuts at:      0     193    2268 
   Size:        193    2075
 
BanII G_rGCy'C

Cuts at:      0    1009    2268 
   Size:       1009    1259
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1097    2268 
   Size:       1097    1171
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1341    2268 
   Size:       1341     927
 
BfrBI ATG'CAT

Cuts at:      0    1183    2268 
   Size:       1183    1085
 
BglII A'GATC_T

Cuts at:      0     213    2268 
   Size:        213    2055
 
Bme1580I G_kGCm'C

Cuts at:      0     923    1943    2268 
   Size:        923    1020     325

  Fragments arranged by size: 

                1020     923     325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     988    1002    1510    2268 
   Size:        988      14     508     758

  Fragments arranged by size: 

                 988     758     508      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     834    2268 
   Size:        834    1434
 
BsaBI GATnn'nnATC

Cuts at:      0     178    2268 
   Size:        178    2090
 
BsaWI w'CCGG_w

Cuts at:      0    2057    2268 
   Size:       2057     211
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     584     614    2268 
   Size:        584      30    1654

  Fragments arranged by size: 

                1654     584      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     498     970    1300    2092    2268 
   Size:        498     472     330     792     176

  Fragments arranged by size: 

                 792     498     472     330     176
 
BseYI C'CCAG_C

Cuts at:      0    1302    2268 
   Size:       1302     966
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     934     997    2268 
   Size:        934      63    1271

  Fragments arranged by size: 

                1271     934      63
 
BsiHKAI G_wGCw'C

Cuts at:      0     923    2268 
   Size:        923    1345
 
BsmI GAATG_Cn'

Cuts at:      0    2041    2268 
   Size:       2041     227
 
Bsp1286I G_dGCh'C

Cuts at:      0     923    1009    1943    2268 
   Size:        923      86     934     325

  Fragments arranged by size: 

                 934     923     325      86
 
BspEI T'CCGG_A

Cuts at:      0    2057    2268 
   Size:       2057     211
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     533    2243    2268 
   Size:        533    1710      25

  Fragments arranged by size: 

                1710     533      25
 
BsrBI CCG'CTC

Cuts at:      0     972    1743    2268 
   Size:        972     771     525

  Fragments arranged by size: 

                 972     771     525
 
BssSI C'ACGA_G

Cuts at:      0     480     952    2268 
   Size:        480     472    1316

  Fragments arranged by size: 

                1316     480     472
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     190    2132    2268 
   Size:        190    1942     136

  Fragments arranged by size: 

                1942     190     136
 
BstYI r'GATC_y

Cuts at:      0     179     213    2268 
   Size:        179      34    2055

  Fragments arranged by size: 

                2055     179      34
 
BtgI C'CryG_G

Cuts at:      0     635    2268 
   Size:        635    1633
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      39    2268 
   Size:         39    2229
 
DraI TTT'AAA

Cuts at:      0     497    2268 
   Size:        497    1771
 
EaeI y'GGCC_r

Cuts at:      0      79     311    2268 
   Size:         79     232    1957

  Fragments arranged by size: 

                1957     232      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     784    1375    2268 
   Size:        784     591     893

  Fragments arranged by size: 

                 893     784     591
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     988    1002    1334    1349    1510    1864    2268 
   Size:        187     801      14     332      15     161     354     404

  Fragments arranged by size: 

                 801     404     354     332     187     161      15      14
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     952    2268 
   Size:        952    1316
 
EcoO109I rG'GnC_Cy

Cuts at:      0     440     459    1387    2268 
   Size:        440      19     928     881

  Fragments arranged by size: 

                 928     881     440      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     786    1834    2268 
   Size:        786    1048     434

  Fragments arranged by size: 

                1048     786     434
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144    1861    1893    1946    1978    2268 
   Size:        112      32    1717      32      53      32     290

  Fragments arranged by size: 

                1717     290     112      53      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2084    2268 
   Size:       2084     184
 
HindIII A'AGCT_T

Cuts at:      0     491    1174    1660    2268 
   Size:        491     683     486     608

  Fragments arranged by size: 

                 683     608     491     486
 
MscI TGG'CCA

Cuts at:      0     313    2268 
   Size:        313    1955
 
MslI CAynn'nnrTG

Cuts at:      0    1987    2268 
   Size:       1987     281
 
MspA1I CmG'CkG

Cuts at:      0      78     358    2268 
   Size:         78     280    1910

  Fragments arranged by size: 

                1910     280      78
 
NsiI A_TGCA'T

Cuts at:      0    1185    2268 
   Size:       1185    1083
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     427     795     906    1146    1783    2268 
   Size:        427     368     111     240     637     485

  Fragments arranged by size: 

                 637     485     427     368     240     111
 
PfoI T'CCnGG_A

Cuts at:      0     965    1018    1526    1562    2131    2268 
   Size:        965      53     508      36     569     137

  Fragments arranged by size: 

                 965     569     508     137      53      36
 
PpuMI rG'GwC_Cy

Cuts at:      0     440     459    1387    2268 
   Size:        440      19     928     881

  Fragments arranged by size: 

                 928     881     440      19
 
PstI C_TGCA'G

Cuts at:      0     257    1193    1476    2225    2268 
   Size:        257     936     283     749      43

  Fragments arranged by size: 

                 936     749     283     257      43
 
PvuII CAG'CTG

Cuts at:      0     358    2268 
   Size:        358    1910
 
SalI G'TCGA_C

Cuts at:      0    2082    2268 
   Size:       2082     186
 
SanDI GG'GwC_CC

Cuts at:      0     440    2268 
   Size:        440    1828
 
SfcI C'TryA_G

Cuts at:      0     253    1189    1472    2221    2268 
   Size:        253     936     283     749      47

  Fragments arranged by size: 

                 936     749     283     253      47
 
SmaI CCC'GGG

Cuts at:      0    1165    2268 
   Size:       1165    1103
 
SmlI C'TyrA_G

Cuts at:      0     813    2268 
   Size:        813    1455
 
SpeI A'CTAG_T

Cuts at:      0     244    1510    2268 
   Size:        244    1266     758

  Fragments arranged by size: 

                1266     758     244
 
SspI AAT'ATT

Cuts at:      0     645    2268 
   Size:        645    1623
 
StuI AGG'CCT

Cuts at:      0     164    2268 
   Size:        164    2104
 
StyI C'CwwG_G

Cuts at:      0    1616    2268 
   Size:       1616     652
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     881    1048    2268 
   Size:        881     167    1220

  Fragments arranged by size: 

                1220     881     167
 
TatI w'GTAC_w

Cuts at:      0     552    1025    2066    2268 
   Size:        552     473    1041     202

  Fragments arranged by size: 

                1041     552     473     202
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     854     886    2268 
   Size:        854      32    1382

  Fragments arranged by size: 

                1382     854      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1101    2268 
   Size:       1101    1167
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1784    2268 
   Size:       1784     484
 
XmaI C'CCGG_G

Cuts at:      0    1163    2268 
   Size:       1163    1105
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AloI    AlwNI    ApaLI     ApoI     AseI
     AvaI     BanI    BanII     BbsI    BciVI    BfrBI    BglII Bme1580I
     BpmI    BpuEI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BssSI    BstXI
    BstYI     BtgI    BtgZI     DraI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FalI    Hin4I   HincII  HindIII     MscI     MslI   MspA1I
     NsiI    PflMI     PfoI    PpuMI     PstI    PvuII     SalI    SanDI
     SfcI     SmaI     SmlI     SpeI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     ApaI     AscI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI     BclI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsiEI    BsiWI
    BsmBI    BspHI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SphI     SrfI     SwaI     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com