Restriction map for pF1KB8556
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AflIII      
AgeI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       AvrII       
BbrPI       BbvCI       BciVI       BclI        BfrI        
BlnI        BsaBI       BsaI        BsiEI       BsiWI       
Bsp1407I    BspDI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SwaI        XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1393  GACGTC               GACGT^C
AccIII       1848  TCCGGA               T^CCGGA
AhdI         1468  GACTTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        245  GTGCAC               G^TGCAC
ApaI          950  GGGCCC               GGGCC^C
ApaLI         245  GTGCAC               G^TGCAC
AspEI        1468  GACTTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI          531  GCACTTGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          355  GCCCACTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         902  AGATCT               A^GATCT
BsaAI         877  TACGTG               [CT]AC^GT[GA]
BsaWI        1848  TCCGGA               [AT]^CCGG[AT]
BseAI        1848  TCCGGA               T^CCGGA
BsgI         1261  GTGCAG               GTGCAG(16/14)
BsmBI        1755  CGTCTC               CGTCTC(1/5)
BsmI         1553  GCATTC               GAATGC(1/-1)
BspEI        1848  TCCGGA               T^CCGGA
BspHI         461  TCATGA               T^CATGA
BsrDI        2591  GCAATG               GCAATG(2/0)
BstAPI       1836  GCAAGAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2097  TTCGAA               TT^CGAA
BstXI         560  CCATGCACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         2515  GACCCATTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1468  GACTTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1231  GAAGAG               CTCTTC(1/4)
EcoNI         582  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       1602  GTCAAC               GT[CT]^[GA]AC
HindII       1602  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1231  GAAGAG               CTCTTC(1/4)
MroI         1848  TCCGGA               T^CCGGA
MslI          941  CACACATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI          945  CATATG               CA^TATG
NspV         2097  TTCGAA               TT^CGAA
PaeR7I       1312  CTCGAG               C^TCGAG
PshAI        1162  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          461  TCATGA               T^CATGA
SacI         1309  GAGCTC               GAGCT^C
SexAI        1694  ACCAGGT              A^CC[AT]GGT
SfuI         2097  TTCGAA               TT^CGAA
SphI          290  GCATGC               GCATG^C
SspI         1199  AATATT               AAT^ATT
StuI         2552  AGGCCT               AGG^CCT
XhoI         1312  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AfaI          573  GTAC                 GT^AC
             2387  GTAC                
AlwNI         495  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2602  CAGCAGCTG           
AspI          442  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1069  GACATGGTC           
BamHI        1851  GGATCC               G^GATCC
             2067  GGATCC              
BbeI            6  GGCGCC               GGCGC^C
              661  GGCGCC              
BfaI          937  CTAG                 C^TAG
             1282  CTAG                
BlpI         1386  GCTGAGC              GC^T[ACGT]AGC
             2703  GCTCAGC             
Bpu1102I     1386  GCTGAGC              GC^T[ACGT]AGC
             2703  GCTCAGC             
BspMI        2349  ACCTGC               ACCTGC(4/8)
               72  GCAGGT              
BsrBI          10  CCGCTC               CCGCTC(-3/-3)
              708  CCGCTC              
CelII        1386  GCTGAGC              GC^T[ACGT]AGC
             2703  GCTCAGC             
DraI         1891  TTTAAA               TTT^AAA
             2308  TTTAAA              
DraIII        248  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              565  CACTTGGTG           
FauI         1077  CCCGC                CCCGC(4/6)
             2622  GCGGG               
HaeII           6  GGCGCC               [GA]GCGC^[CT]
              661  GGCGCC              
HgaI          731  GCGTC                GACGC(5/10)
             1754  GCGTC               
KasI            6  GGCGCC               G^GCGCC
              661  GGCGCC              
MaeI          937  CTAG                 C^TAG
             1282  CTAG                
MaeII         878  ACGT                 A^CGT
             1394  ACGT                
NarI            6  GGCGCC               GG^CGCC
              661  GGCGCC              
NcoI         1713  CCATGG               C^CATGG
             1787  CCATGG              
PflFI         442  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1069  GACATGGTC           
PflMI        2002  CCATTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2251  CCACCTTCTGG         
PleI         1419  GACTC                GAGTC(4/5)
             1568  GAGTC               
PstI          347  CTGCAG               CTGCA^G
             2534  CTGCAG              
RsaI          573  GTAC                 GT^AC
             2387  GTAC                
SfcI          347  CTGCAG               C^T[GA][CT]AG
             2534  CTGCAG              
SfoI            6  GGCGCC               GGC^GCC
              661  GGCGCC              
Tth111I       442  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1069  GACATGGTC           
Van91I       2002  CCATTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2251  CCACCTTCTGG         
XcmI          793  CCAACAGTGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1695  CCAGGTGCTCGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PpuMI       PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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