Restriction map for pF1KB8521
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccI        AclI        AgeI        AhdI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BglII       
BlnI        BsaBI       BsiEI       BsiWI       Bsp1407I    
BspDI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        MaeI        MamI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        SalI        
SapI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflII        1141  CTTAAG               C^TTAAG
AflIII        155  ACATGT               A^C[GA][CT]GT
Alw44I       2458  GTGCAC               G^TGCAC
AocI         1551  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2027  AGCGCT               AGC^GCT
ApaLI        2458  GTGCAC               G^TGCAC
AviII        2086  TGCGCA               TGC^GCA
BfrI         1141  CTTAAG               C^TTAAG
BlpI         2295  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2295  GCTCAGC              GC^T[ACGT]AGC
BsmBI        2192  GAGACG               CGTCTC(1/5)
BsmI         1584  GAATGC               GAATGC(1/-1)
BspHI        2645  TCATGA               T^CATGA
BspLU11I      155  ACATGT               A^CATGT
BsrDI        2234  GCAATG               GCAATG(2/0)
BssHII       1745  GCGCGC               G^CGCGC
BssSI        1335  CACGAG               CACGAG(-5/-1)
BstAPI       2624  GCAGCATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        2201  AGATCC               [GA]^GATC[CT]
Bsu36I       1551  CCTGAGG              CC^T[ACGT]AGG
CelII        2295  GCTCAGC              GC^T[ACGT]AGC
CpoI         1450  CGGACCG              CG^G[AT]CCG
DraIII       2855  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2027  AGCGCT               AGC^GCT
Eco81I       1551  CCTGAGG              CC^T[ACGT]AGG
FspI         2086  TGCGCA               TGC^GCA
HincII        274  GTCAAC               GT[CT]^[GA]AC
HindII        274  GTCAAC               GT[CT]^[GA]AC
HphI         2636  TCACC                GGTGA(8/7)
KspI         2317  CCGCGG               CCGC^GG
MflI         2201  AGATCC               [GA]^GATC[CT]
PflMI        1994  CCAAGGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         285  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI         2645  TCATGA               T^CATGA
RsrII        1450  CGGACCG              CG^G[AT]CCG
SacI          861  GAGCTC               GAGCT^C
SacII        2317  CCGCGG               CCGC^GG
SfiI         1089  GGCCAAGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Van91I       1994  CCAAGGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2775  CCAAGCCCCGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        2201  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I       1714  GGTACC               G^GTACC
             1916  GGTACC              
AccIII       1558  TCCGGA               T^CCGGA
             2797  TCCGGA              
AcsI          408  AAATTC               [GA]^AATT[CT]
              970  AAATTC              
AlwI         2202  GATCC                GGATC(4/5)
              465  GGATC               
ApaI         1760  GGGCCC               GGGCC^C
             2578  GGGCCC              
ApoI          408  AAATTC               [GA]^AATT[CT]
              970  AAATTC              
Asp718I      1714  GGTACC               G^GTACC
             1916  GGTACC              
BalI          330  TGGCCA               TGG^CCA
              444  TGGCCA              
BbeI           24  GGCGCC               GGCGC^C
               31  GGCGCC              
BsaAI        1049  TACGTG               [CT]AC^GT[GA]
             2455  TACGTG              
BsaWI        1558  TCCGGA               [AT]^CCGG[AT]
             2797  TCCGGA              
BseAI        1558  TCCGGA               T^CCGGA
             2797  TCCGGA              
BspEI        1558  TCCGGA               T^CCGGA
             2797  TCCGGA              
BstXI          47  CCACAGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1373  CCAAGACCCTGG        
EcoNI        1920  CCTGTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2013  CCTGGCTAAGG         
KasI           24  GGCGCC               G^GCGCC
               31  GGCGCC              
KpnI         1714  GGTACC               GGTAC^C
             1916  GGTACC              
MluNI         330  TGGCCA               TGG^CCA
              444  TGGCCA              
MroI         1558  TCCGGA               T^CCGGA
             2797  TCCGGA              
MscI          330  TGGCCA               TGG^CCA
              444  TGGCCA              
MslI           48  CACAGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2230  CACGGCAATG          
NarI           24  GGCGCC               GG^CGCC
               31  GGCGCC              
NcoI           53  CCATGG               C^CATGG
             1911  CCATGG              
NspI          155  ACATGT               [GA]CATG^[CT]
             2627  GCATGT              
PvuII        1480  CAGCTG               CAG^CTG
             2212  CAGCTG              
SfoI           24  GGCGCC               GGC^GCC
               31  GGCGCC              
StuI          206  AGGCCT               AGG^CCT
             2171  AGGCCT              
TaqI          317  TCGA                 T^CGA
              367  TCGA                
Tsp45I       1939  GTGAC                ^GT[GC]AC
             2524  GTGAC               
TthHB8I       317  TCGA                 T^CGA
              367  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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