Restriction map for pF1KB8521
 (Linear) (Six-base) MAPSORT of: KIBB8521.seq  Check: 4990  from: 1  to: 2868



KIBB8521.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2457    2504    2868 
   Size:       2457      47     364

  Fragments arranged by size: 

                2457     364      47
 
Acc65I G'GTAC_C

Cuts at:      0    1714    1916    2868 
   Size:       1714     202     952

  Fragments arranged by size: 

                1714     952     202
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    2008    2122    2424    2626    2702    2868 
   Size:        434    1574     114     302     202      76     166

  Fragments arranged by size: 

                1574     434     302     202     166     114      76
 
AfeI AGC'GCT

Cuts at:      0    2029    2868 
   Size:       2029     839
 
AflII C'TTAA_G

Cuts at:      0    1141    2868 
   Size:       1141    1727
 
AflIII A'CryG_T

Cuts at:      0     155    2868 
   Size:        155    2713
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1551    1986    2084    2205    2217    2868 
   Size:       1551     435      98     121      12     651

  Fragments arranged by size: 

                1551     651     435     121      98      12
 
ApaI G_GGCC'C

Cuts at:      0    1764    2582    2868 
   Size:       1764     818     286

  Fragments arranged by size: 

                1764     818     286
 
ApaLI G'TGCA_C

Cuts at:      0    2458    2868 
   Size:       2458     410
 
ApoI r'AATT_y

Cuts at:      0     408     970    2868 
   Size:        408     562    1898

  Fragments arranged by size: 

                1898     562     408
 
AvaI C'yCGr_G

Cuts at:      0     639     886    1645    2868 
   Size:        639     247     759    1223

  Fragments arranged by size: 

                1223     759     639     247
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     164     197     906     939    1698    1731    2868 
   Size:        164      33     709      33     759      33    1137

  Fragments arranged by size: 

                1137     759     709     164      33      33      33
 
BanI G'GyrC_C

Cuts at:      0      24      31     708    1714    1916    2532    2868 
   Size:         24       7     677    1006     202     616     336

  Fragments arranged by size: 

                1006     677     616     336     202      24       7
 
BanII G_rGCy'C

Cuts at:      0     865    1764    2326    2582    2854    2868 
   Size:        865     899     562     256     272      14

  Fragments arranged by size: 

                 899     865     562     272     256      14
 
BbeI G_GCGC'C

Cuts at:      0      28      35    2868 
   Size:         28       7    2833

  Fragments arranged by size: 

                2833      28       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     461     839    1735    2690    2868 
   Size:        461     378     896     955     178

  Fragments arranged by size: 

                 955     896     461     378     178
 
BglI GCCn_nnn'nGGC

Cuts at:      0     891    1096    1246    2868 
   Size:        891     205     150    1622

  Fragments arranged by size: 

                1622     891     205     150
 
BlpI GC'TnA_GC

Cuts at:      0    2296    2868 
   Size:       2296     572
 
Bme1580I G_kGCm'C

Cuts at:      0     652    1764    1910    2462    2537    2582    2593    2868 
   Size:        652    1112     146     552      75      45      11     275

  Fragments arranged by size: 

                1112     652     552     275     146      75      45      11
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     470    1719    2866    2868 
   Size:        470    1249    1147       2

  Fragments arranged by size: 

                1249    1147     470       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      34     411     536    1264    1480    2155    2868 
   Size:         25       9     377     125     728     216     675     713

  Fragments arranged by size: 

                 728     713     675     377     216     125      25       9
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     859    2018    2868 
   Size:        859    1159     850

  Fragments arranged by size: 

                1159     859     850
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1192    1276    2377    2868 
   Size:       1027     165      84    1101     491

  Fragments arranged by size: 

                1101    1027     491     165      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     514     738    1745    2131    2868 
   Size:        514     224    1007     386     737

  Fragments arranged by size: 

                1007     737     514     386     224
 
BsaAI yAC'GTr

Cuts at:      0    1051    2457    2868 
   Size:       1051    1406     411

  Fragments arranged by size: 

                1406    1051     411
 
BsaHI Gr'CG_yC

Cuts at:      0      25      32    1446    2164    2868 
   Size:         25       7    1414     718     704

  Fragments arranged by size: 

                1414     718     704      25       7
 
BsaWI w'CCGG_w

Cuts at:      0    1558    2797    2868 
   Size:       1558    1239      71

  Fragments arranged by size: 

                1558    1239      71
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       1      31    1822    1852    2868 
   Size:          1      30    1791      30    1016

  Fragments arranged by size: 

                1791    1016      30      30       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     565     568     597    1099    1120    1228    1483 
   Size:         22     543       3      29     502      21     108     255

Cuts at:   1483    1665    1819    1834    1960    2170    2202    2728    2731 
   Size:        182     154      15     126     210      32     526       3

Cuts at:   2731    2740    2743    2868 
   Size:          9       3     125

  Fragments arranged by size: 

                 543     526     502     255     210     182     154     126
                 125     108      32      29      22      21      15       9
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     682    2210    2356    2581    2803    2868 
   Size:        682    1528     146     225     222      65

  Fragments arranged by size: 

                1528     682     225     222     146      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596    1073    2212    2326    2868 
   Size:        596     477    1139     114     542

  Fragments arranged by size: 

                1139     596     542     477     114
 
BsiHKAI G_wGCw'C

Cuts at:      0     340     865    1427    2279    2462    2495    2868 
   Size:        340     525     562     852     183      33     373

  Fragments arranged by size: 

                 852     562     525     373     340     183      33
 
BsmI GAATG_Cn'

Cuts at:      0    1590    2868 
   Size:       1590    1278
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2186    2868 
   Size:       2186     682
 
Bsp1286I G_dGCh'C

Cuts at:      0     340     652     865    1427    1764    1910    2279    2326 
   Size:        340     312     213     562     337     146     369      47

Cuts at:   2326    2462    2495    2537    2582    2593    2854    2868 
   Size:        136      33      42      45      11     261      14

  Fragments arranged by size: 

                 562     369     340     337     312     261     213     146
                 136      47      45      42      33      14      11
 
BspEI T'CCGG_A

Cuts at:      0    1558    2797    2868 
   Size:       1558    1239      71

  Fragments arranged by size: 

                1558    1239      71
 
BspHI T'CATG_A

Cuts at:      0    2645    2868 
   Size:       2645     223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2348    2457    2504    2868 
   Size:       2348     109      47     364

  Fragments arranged by size: 

                2348     364     109      47
 
BsrBI CCG'CTC

Cuts at:      0    1218    1508    2223    2868 
   Size:       1218     290     715     645

  Fragments arranged by size: 

                1218     715     645     290
 
BsrDI GCAATG_nn'

Cuts at:      0    2241    2868 
   Size:       2241     627
 
BsrFI r'CCGG_y

Cuts at:      0    1384    1541    1697    2145    2308    2827    2868 
   Size:       1384     157     156     448     163     519      41

  Fragments arranged by size: 

                1384     519     448     163     157     156      41
 
BssHII G'CGCG_C

Cuts at:      0    1745    2868 
   Size:       1745    1123
 
BssSI C'ACGA_G

Cuts at:      0    1335    2868 
   Size:       1335    1533
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2630    2868 
   Size:       2630     238
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      54    1380    2868 
   Size:         54    1326    1488

  Fragments arranged by size: 

                1488    1326      54
 
BstYI r'GATC_y

Cuts at:      0    2201    2868 
   Size:       2201     667
 
Bsu36I CC'TnA_GG

Cuts at:      0    1552    2868 
   Size:       1552    1316
 
BtgI C'CryG_G

Cuts at:      0      53     610    1911    2317    2573    2868 
   Size:         53     557    1301     406     256     295

  Fragments arranged by size: 

                1301     557     406     295     256      53
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2108    2868 
   Size:       2108     760
 
BtsI GCAGTG_nn'

Cuts at:      0    2277    2868 
   Size:       2277     591
 
DraIII CAC_nnn'GTG

Cuts at:      0    2860    2868 
   Size:       2860       8
 
EaeI y'GGCC_r

Cuts at:      0     330     444    1382    2667    2868 
   Size:        330     114     938    1285     201

  Fragments arranged by size: 

                1285     938     330     201     114
 
EarI CTCTTCn'nnn_

Cuts at:      0     876    1124    1274    1295    1811    2602    2717    2868 
   Size:        876     248     150      21     516     791     115     151

  Fragments arranged by size: 

                 876     791     516     248     151     150     115      21
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1207    1966    2868 
   Size:       1207     759     902

  Fragments arranged by size: 

                1207     902     759
 
EcoICRI GAG'CTC

Cuts at:      0     863    2868 
   Size:        863    2005
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      34     411     434     536    1264    1480    2008 
   Size:         25       9     377      23     102     728     216     528

Cuts at:   2008    2122    2155    2424    2626    2702    2868 
   Size:        114      33     269     202      76     166

  Fragments arranged by size: 

                 728     528     377     269     216     202     166     114
                 102      76      33      25      23       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1924    2017    2868 
   Size:       1924      93     851

  Fragments arranged by size: 

                1924     851      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     286    1368    2578    2868 
   Size:        286    1082    1210     290

  Fragments arranged by size: 

                1210    1082     290     286
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     306    2868 
   Size:        306    2562
 
FspI TGC'GCA

Cuts at:      0    2088    2868 
   Size:       2088     780
 
HaeII r_GCGC'y

Cuts at:      0      28      35     123    2031    2868 
   Size:         28       7      88    1908     837

  Fragments arranged by size: 

                1908     837      88      28       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174    1822    1854    2868 
   Size:        142      32    1648      32    1014

  Fragments arranged by size: 

                1648    1014     142      32      32
 
HincII GTy'rAC

Cuts at:      0     276    2868 
   Size:        276    2592
 
KasI G'GCGC_C

Cuts at:      0      24      31    2868 
   Size:         24       7    2837

  Fragments arranged by size: 

                2837      24       7
 
KpnI G_GTAC'C

Cuts at:      0    1718    1920    2868 
   Size:       1718     202     948

  Fragments arranged by size: 

                1718     948     202
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     652    1429    2868 
   Size:        652     777    1439

  Fragments arranged by size: 

                1439     777     652
 
MscI TGG'CCA

Cuts at:      0     332     446    2868 
   Size:        332     114    2422

  Fragments arranged by size: 

                2422     332     114
 
MslI CAynn'nnrTG

Cuts at:      0      52    2234    2868 
   Size:         52    2182     634

  Fragments arranged by size: 

                2182     634      52
 
MspA1I CmG'CkG

Cuts at:      0    1203    1482    2214    2319    2620    2868 
   Size:       1203     279     732     105     301     248

  Fragments arranged by size: 

                1203     732     301     279     248     105
 
NaeI GCC'GGC

Cuts at:      0    1386    1543    2147    2868 
   Size:       1386     157     604     721

  Fragments arranged by size: 

                1386     721     604     157
 
NarI GG'CG_CC

Cuts at:      0      25      32    2868 
   Size:         25       7    2836

  Fragments arranged by size: 

                2836      25       7
 
NcoI C'CATG_G

Cuts at:      0      53    1911    2868 
   Size:         53    1858     957

  Fragments arranged by size: 

                1858     957      53
 
NgoMIV G'CCGG_C

Cuts at:      0    1384    1541    2145    2868 
   Size:       1384     157     604     723

  Fragments arranged by size: 

                1384     723     604     157
 
NspI r_CATG'y

Cuts at:      0     159    2631    2868 
   Size:        159    2472     237

  Fragments arranged by size: 

                2472     237     159
 
PasI CC'CwG_GG

Cuts at:      0    1757    2868 
   Size:       1757    1111
 
PciI A'CATG_T

Cuts at:      0     155    2868 
   Size:        155    2713
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2000    2868 
   Size:       2000     868
 
PfoI T'CCnGG_A

Cuts at:      0     513    1778    2132    2261    2270    2756    2868 
   Size:        513    1265     354     129       9     486     112

  Fragments arranged by size: 

                1265     513     486     354     129     112       9
 
PpuMI rG'GwC_Cy

Cuts at:      0     286    2868 
   Size:        286    2582
 
PspOMI G'GGCC_C

Cuts at:      0    1760    2578    2868 
   Size:       1760     818     290

  Fragments arranged by size: 

                1760     818     290
 
PstI C_TGCA'G

Cuts at:      0    1477    1682    2114    2868 
   Size:       1477     205     432     754

  Fragments arranged by size: 

                1477     754     432     205
 
PvuII CAG'CTG

Cuts at:      0    1482    2214    2868 
   Size:       1482     732     654

  Fragments arranged by size: 

                1482     732     654
 
RsrII CG'GwC_CG

Cuts at:      0    1451    2868 
   Size:       1451    1417
 
SacI G_AGCT'C

Cuts at:      0     865    2868 
   Size:        865    2003
 
SacII CC_GC'GG

Cuts at:      0    2320    2868 
   Size:       2320     548
 
SanDI GG'GwC_CC

Cuts at:      0     286    2868 
   Size:        286    2582
 
SfcI C'TryA_G

Cuts at:      0     986    1473    1678    2110    2473    2868 
   Size:        986     487     205     432     363     395

  Fragments arranged by size: 

                 986     487     432     395     363     205
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1096    2868 
   Size:       1096    1772
 
SfoI GGC'GCC

Cuts at:      0      26      33    2868 
   Size:         26       7    2835

  Fragments arranged by size: 

                2835      26       7
 
SmlI C'TyrA_G

Cuts at:      0    1042    1141    1207    1255    2392    2868 
   Size:       1042      99      66      48    1137     476

  Fragments arranged by size: 

                1137    1042     476      99      66      48
 
StuI AGG'CCT

Cuts at:      0     208    2173    2868 
   Size:        208    1965     695

  Fragments arranged by size: 

                1965     695     208
 
StyI C'CwwG_G

Cuts at:      0      53     221     780    1100    1911    1994    2168    2868 
   Size:         53     168     559     320     811      83     174     700

  Fragments arranged by size: 

                 811     700     559     320     174     168      83      53
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     279    2653    2848    2868 
   Size:        279    2374     195      20

  Fragments arranged by size: 

                2374     279     195      20
 
TatI w'GTAC_w

Cuts at:      0     840    2868 
   Size:        840    2028
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       1      33    2868 
   Size:          1      32    2835

  Fragments arranged by size: 

                2835      32       1
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2782    2868 
   Size:       2782      86
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI    AflII   AflIII    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbeI     BbsI
     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII    RsrII     SacI    SacII    SanDI     SfcI     SfiI
     SfoI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AgeI     AhdI     AleI     AlfI     AloI
     AscI     AseI    AsiSI    AvrII    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmtI     BplI    BsaBI    BsiEI
    BsiWI    BsrGI    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI
     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
     SalI     SapI     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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