Restriction map for pF1KB5718
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BanI        
BanII       BbeI        BbvCI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspLU11I    BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       FauI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
StuI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII         25  TCCGGA               T^CCGGA
AflIII       1075  ACGTGT               A^C[GA][CT]GT
AvaII        1493  GGTCC                G^G[AT]CC
BamHI         534  GGATCC               G^GATCC
BbrPI         740  CACGTG               CAC^GTG
BcgI         1132  GCAGGGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          743  GTGCCC               G[AGT]GC[ACT]^C
BseAI          25  TCCGGA               T^CCGGA
Bsp1286I      743  GTGCCC               G[AGT]GC[ACT]^C
BspEI          25  TCCGGA               T^CCGGA
BspHI        1053  TCATGA               T^CATGA
BspMI         779  ACCTGC               ACCTGC(4/8)
BsrBI           3  GAGCGG               CCGCTC(-3/-3)
BsrDI         638  GCAATG               GCAATG(2/0)
BssSI        1226  CACGAG               CACGAG(-5/-1)
CfoI         1684  GCGC                 GCG^C
Cfr13I       1493  GGTCC                G^G[ACGT]CC
EcoNI        1126  CCTACAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         256  GATATC               GAT^ATC
EcoT22I        71  ATGCAT               ATGCA^T
HaeII        1683  AGCGCC               [GA]GCGC^[CT]
HhaI         1684  GCGC                 GCG^C
HinP1I       1684  GCGC                 G^CGC
MroI           25  TCCGGA               T^CCGGA
MslI         1837  CACGACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        404  CAGCTG               C[AC]G^C[GT]G
NcoI         1617  CCATGG               C^CATGG
NdeI         1480  CATATG               CA^TATG
NsiI           71  ATGCAT               ATGCA^T
PaeR7I       1763  CTCGAG               C^TCGAG
PflMI         931  CCAACACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         740  CACGTG               CAC^GTG
PmlI          740  CACGTG               CAC^GTG
PvuII         404  CAGCTG               CAG^CTG
RcaI         1053  TCATGA               T^CATGA
Sau96I       1493  GGTCC                G^G[ACGT]CC
ScaI          829  AGTACT               AGT^ACT
SexAI         936  ACCTGGT              A^CC[AT]GGT
SfcI         1127  CTACAG               C^T[GA][CT]AG
SmaI         2108  CCCGGG               CCC^GGG
SphI          597  GCATGC               GCATG^C
SspI          137  AATATT               AAT^ATT
SwaI          182  ATTTAAAT             ATTT^AAAT
Van91I        931  CCAACACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1763  CTCGAG               C^TCGAG
XmaCI        2108  CCCGGG               C^CCGGG
XmaI         2108  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          186  AAATTT               [GA]^AATT[CT]
             1598  AAATTT              
AfaI          830  GTAC                 GT^AC
             2105  GTAC                
AlwNI        1100  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1523  CAGTTCCTG           
ApoI          186  AAATTT               [GA]^AATT[CT]
             1598  AAATTT              
AvaI         1763  CTCGAG               C^[CT]CG[GA]G
             2108  CCCGGG              
BbsI         1885  GAAGAC               GAAGAC(2/6)
             2085  GAAGAC              
BciVI        1082  GTATCC               GTATCC(6/5)
             1121  GTATCC              
BclI          150  TGATCA               T^GATCA
             1937  TGATCA              
BpuAI        1885  GAAGAC               GAAGAC(2/6)
             2085  GAAGAC              
BsaWI          25  TCCGGA               [AT]^CCGG[AT]
              272  ACCGGA              
BsmI         1457  GAATGC               GAATGC(1/-1)
              524  GCATTC              
BsoBI        1763  CTCGAG               C^[CT]CG[GA]G
             2108  CCCGGG              
DsaI         1553  CCGTGG               C^C[GA][CT]GG
             1617  CCATGG              
EaeI          105  TGGCCG               [CT]^GGCC[GA]
             1030  CGGCCA              
FbaI          150  TGATCA               T^GATCA
             1937  TGATCA              
NspI          597  GCATGC               [GA]CATG^[CT]
              903  GCATGT              
RsaI          830  GTAC                 GT^AC
             2105  GTAC                
SfaNI         205  GATGC                GCATC(5/9)
             1195  GATGC               
XcmI          678  CCAGAGCAAACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1160  CCATTTCCACTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AluI        AlwI        Asp700I     BbvI        
BcnI        BfaI        BglII       BpmI        BsaAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       DdeI        DpnII       DraI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      ScrFI       SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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