Restriction map for pF1KB5718
 (Linear) (Six-base) MAPSORT of: KIBB5718.seq  Check: 8181  from: 1  to: 2172



KIBB5718.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740     854    1473    2172 
   Size:        740     114     619     699

  Fragments arranged by size: 

                 740     699     619     114
 
AflIII A'CryG_T

Cuts at:      0    1075    2172 
   Size:       1075    1097
 
AleI CACnn'nnGTG

Cuts at:      0    1841    2172 
   Size:       1841     331
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1448    1480    2172 
   Size:       1448      32     692

  Fragments arranged by size: 

                1448     692      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1105    1528    2172 
   Size:       1105     423     644

  Fragments arranged by size: 

                1105     644     423
 
ApoI r'AATT_y

Cuts at:      0     186    1598    2172 
   Size:        186    1412     574

  Fragments arranged by size: 

                1412     574     186
 
AvaI C'yCGr_G

Cuts at:      0    1763    2108    2172 
   Size:       1763     345      64

  Fragments arranged by size: 

                1763     345      64
 
BamHI G'GATC_C

Cuts at:      0     534    2172 
   Size:        534    1638
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1892    2092    2172 
   Size:       1892     200      80

  Fragments arranged by size: 

                1892     200      80
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1121    1155    2172 
   Size:       1121      34    1017

  Fragments arranged by size: 

                1121    1017      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1093    1132    2172 
   Size:       1093      39    1040

  Fragments arranged by size: 

                1093    1040      39
 
BclI T'GATC_A

Cuts at:      0     150    1937    2172 
   Size:        150    1787     235

  Fragments arranged by size: 

                1787     235     150
 
BfrBI ATG'CAT

Cuts at:      0      73    2172 
   Size:         73    2099
 
BglII A'GATC_T

Cuts at:      0    1246    1738    1974    2172 
   Size:       1246     492     236     198

  Fragments arranged by size: 

                1246     492     236     198
 
Bme1580I G_kGCm'C

Cuts at:      0     747    2172 
   Size:        747    1425
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1726    2172 
   Size:       1726     446
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1778    2172 
   Size:       1778     394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     750    1412    2172 
   Size:        137     613     662     760

  Fragments arranged by size: 

                 760     662     613     137
 
Bpu10I CC'TnA_GC

Cuts at:      0    2031    2172 
   Size:       2031     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1190    1552    2172 
   Size:        524     666     362     620

  Fragments arranged by size: 

                 666     620     524     362
 
BsaAI yAC'GTr

Cuts at:      0     742    1150    2011    2172 
   Size:        742     408     861     161

  Fragments arranged by size: 

                 861     742     408     161
 
BsaWI w'CCGG_w

Cuts at:      0      25     272    2172 
   Size:         25     247    1900

  Fragments arranged by size: 

                1900     247      25
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      43      73     459     489    2172 
   Size:         43      30     386      30    1683

  Fragments arranged by size: 

                1683     386      43      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      96     316     319     467    1460    2172 
   Size:         96     220       3     148     993     712

  Fragments arranged by size: 

                 993     712     220     148      96       3
 
BsmI GAATG_Cn'

Cuts at:      0     524    1463    2172 
   Size:        524     939     709

  Fragments arranged by size: 

                 939     709     524
 
Bsp1286I G_dGCh'C

Cuts at:      0     747    2172 
   Size:        747    1425
 
BspEI T'CCGG_A

Cuts at:      0      25    2172 
   Size:         25    2147
 
BspHI T'CATG_A

Cuts at:      0    1053    2172 
   Size:       1053    1119
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     788    2172 
   Size:        788    1384
 
BsrBI CCG'CTC

Cuts at:      0       5    2172 
   Size:          5    2167
 
BsrDI GCAATG_nn'

Cuts at:      0     645    2172 
   Size:        645    1527
 
BssSI C'ACGA_G

Cuts at:      0    1226    2172 
   Size:       1226     946
 
BstYI r'GATC_y

Cuts at:      0     120     366     534    1246    1738    1974    2172 
   Size:        120     246     168     712     492     236     198

  Fragments arranged by size: 

                 712     492     246     236     198     168     120
 
BtgI C'CryG_G

Cuts at:      0    1553    1617    2172 
   Size:       1553      64     555

  Fragments arranged by size: 

                1553     555      64
 
BtsI GCAGTG_nn'

Cuts at:      0     108    1833    2031    2046    2172 
   Size:        108    1725     198      15     126

  Fragments arranged by size: 

                1725     198     126     108      15
 
DraI TTT'AAA

Cuts at:      0     185    1178    1281    2172 
   Size:        185     993     103     891

  Fragments arranged by size: 

                 993     891     185     103
 
EaeI y'GGCC_r

Cuts at:      0     105    1030    2172 
   Size:        105     925    1142

  Fragments arranged by size: 

                1142     925     105
 
EarI CTCTTCn'nnn_

Cuts at:      0      73     627    1776    1841    2155    2172 
   Size:         73     554    1149      65     314      17

  Fragments arranged by size: 

                1149     554     314      73      65      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     740     750     854    1412    1473    2172 
   Size:        137     603      10     104     558      61     699

  Fragments arranged by size: 

                 699     603     558     137     104      61      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1130    2172 
   Size:       1130    1042
 
EcoRV GAT'ATC

Cuts at:      0     258    2172 
   Size:        258    1914
 
HaeII r_GCGC'y

Cuts at:      0    1687    2172 
   Size:       1687     485
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477    1432    1464    1730    1762    2079    2111 
   Size:        445      32     955      32     266      32     317      32

Cuts at:   2111    2172 
   Size:         61

  Fragments arranged by size: 

                 955     445     317     266      61      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     196     762    1298    2076    2172 
   Size:        196     566     536     778      96

  Fragments arranged by size: 

                 778     566     536     196      96
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     955    2172 
   Size:        379     576    1217

  Fragments arranged by size: 

                1217     576     379
 
MslI CAynn'nnrTG

Cuts at:      0    1841    2172 
   Size:       1841     331
 
MspA1I CmG'CkG

Cuts at:      0     406    2172 
   Size:        406    1766
 
NcoI C'CATG_G

Cuts at:      0    1617    2172 
   Size:       1617     555
 
NdeI CA'TA_TG

Cuts at:      0    1481    2172 
   Size:       1481     691
 
NsiI A_TGCA'T

Cuts at:      0      75    2172 
   Size:         75    2097
 
NspI r_CATG'y

Cuts at:      0     601     907    2172 
   Size:        601     306    1265

  Fragments arranged by size: 

                1265     601     306
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     937    2172 
   Size:        937    1235
 
PfoI T'CCnGG_A

Cuts at:      0    1428    2172 
   Size:       1428     744
 
PmlI CAC'GTG

Cuts at:      0     742    2172 
   Size:        742    1430
 
PsiI TTA'TAA

Cuts at:      0     776    2172 
   Size:        776    1396
 
PvuII CAG'CTG

Cuts at:      0     406    2172 
   Size:        406    1766
 
ScaI AGT'ACT

Cuts at:      0     831    2172 
   Size:        831    1341
 
SexAI A'CCwGG_T

Cuts at:      0     936    2172 
   Size:        936    1236
 
SfcI C'TryA_G

Cuts at:      0    1127    2172 
   Size:       1127    1045
 
SmaI CCC'GGG

Cuts at:      0    2110    2172 
   Size:       2110      62
 
SmlI C'TyrA_G

Cuts at:      0     539    1205    1567    1763    2172 
   Size:        539     666     362     196     409

  Fragments arranged by size: 

                 666     539     409     362     196
 
SphI G_CATG'C

Cuts at:      0     601    2172 
   Size:        601    1571
 
SspI AAT'ATT

Cuts at:      0     139    2172 
   Size:        139    2033
 
StyI C'CwwG_G

Cuts at:      0     980    1617    2090    2172 
   Size:        980     637     473      82

  Fragments arranged by size: 

                 980     637     473      82
 
SwaI ATTT'AAAT

Cuts at:      0     185    2172 
   Size:        185    1987
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     460    1073    2172 
   Size:        460     613    1099

  Fragments arranged by size: 

                1099     613     460
 
TatI w'GTAC_w

Cuts at:      0     829    2172 
   Size:        829    1343
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     867     899    2172 
   Size:        867      32    1273

  Fragments arranged by size: 

                1273     867      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     685    1167    2172 
   Size:        685     482    1005

  Fragments arranged by size: 

                1005     685     482
 
XhoI C'TCGA_G

Cuts at:      0    1763    2172 
   Size:       1763     409
 
XmaI C'CCGG_G

Cuts at:      0    2108    2172 
   Size:       2108      64
 
XmnI GAAnn'nnTTC

Cuts at:      0    1311    1397    1635    2172 
   Size:       1311      86     238     537

  Fragments arranged by size: 

                1311     537     238      86
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AloI    AlwNI     ApoI     AvaI    BamHI
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseRI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BssSI
    BstYI     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI
    EcoRV    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI    PflMI     PfoI     PmlI     PsiI    PvuII
     ScaI    SexAI     SfcI     SmaI     SmlI     SphI     SspI     StyI
     SwaI    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI    BanII     BbeI    BbvCI     BglI     BlpI    BmgBI
     BmtI     BsaI    BsaBI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     StuI  Tth111I     XbaI     ZraI
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