Restriction map for pF1KB8515
 (Linear) (Six-base) MAPSORT of: KIBB8515.seq  Check: 4749  from: 1  to: 2844



KIBB8515.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     382    2844 
   Size:        382    2462
 
AatII G_ACGT'C

Cuts at:      0    1234    2720    2844 
   Size:       1234    1486     124

  Fragments arranged by size: 

                1486    1234     124
 
AccI GT'mk_AC

Cuts at:      0    2228    2844 
   Size:       2228     616
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     193     454     900    1378    1975    2260    2622 
   Size:        187       6     261     446     478     597     285     362

Cuts at:   2622    2844 
   Size:        222

  Fragments arranged by size: 

                 597     478     446     362     285     261     222     187
                   6
 
AfeI AGC'GCT

Cuts at:      0     738    2844 
   Size:        738    2106
 
AflII C'TTAA_G

Cuts at:      0    1201    2844 
   Size:       1201    1643
 
AflIII A'CryG_T

Cuts at:      0    2087    2147    2384    2844 
   Size:       2087      60     237     460

  Fragments arranged by size: 

                2087     460     237      60
 
AleI CACnn'nnGTG

Cuts at:      0    1219    1561    2844 
   Size:       1219     342    1283

  Fragments arranged by size: 

                1283    1219     342
 
AlwNI CAG_nnn'CTG

Cuts at:      0      33     120     288     297     807    2844 
   Size:         33      87     168       9     510    2037

  Fragments arranged by size: 

                2037     510     168      87      33       9
 
ApaI G_GGCC'C

Cuts at:      0     232     938    2844 
   Size:        232     706    1906

  Fragments arranged by size: 

                1906     706     232
 
ApoI r'AATT_y

Cuts at:      0    1351    1493    1915    2844 
   Size:       1351     142     422     929

  Fragments arranged by size: 

                1351     929     422     142
 
AvaI C'yCGr_G

Cuts at:      0     103    1112    1777    1976    2844 
   Size:        103    1009     665     199     868

  Fragments arranged by size: 

                1009     868     665     199     103
 
BamHI G'GATC_C

Cuts at:      0    1633    1722    2844 
   Size:       1633      89    1122

  Fragments arranged by size: 

                1633    1122      89
 
BanI G'GyrC_C

Cuts at:      0     674     709    1606    2672    2844 
   Size:        674      35     897    1066     172

  Fragments arranged by size: 

                1066     897     674     172      35
 
BanII G_rGCy'C

Cuts at:      0     232     256     893     938    1717    1753    2162    2767 
   Size:        232      24     637      45     779      36     409     605

Cuts at:   2767    2844 
   Size:         77

  Fragments arranged by size: 

                 779     637     605     409     232      77      45      36
                  24
 
BbeI G_GCGC'C

Cuts at:      0    2676    2844 
   Size:       2676     168
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2478    2844 
   Size:       2478     366
 
BbvCI CC'TCA_GC

Cuts at:      0    1433    2844 
   Size:       1433    1411
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2590    2624    2844 
   Size:       2590      34     220

  Fragments arranged by size: 

                2590     220      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1145    1948    2665    2844 
   Size:       1145     803     717     179

  Fragments arranged by size: 

                1145     803     717     179
 
BlpI GC'TnA_GC

Cuts at:      0     733    2844 
   Size:        733    2111
 
Bme1580I G_kGCm'C

Cuts at:      0     232     938    1296    1347    1603    2696    2844 
   Size:        232     706     358      51     256    1093     148

  Fragments arranged by size: 

                1093     706     358     256     232     148      51
 
BmgBI CAC'GTC

Cuts at:      0    2148    2387    2844 
   Size:       2148     239     457

  Fragments arranged by size: 

                2148     457     239
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     342    1919    2428    2844 
   Size:        342    1577     509     416

  Fragments arranged by size: 

                1577     509     416     342
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      62     907    1292    1487    2844 
   Size:         62     845     385     195    1357

  Fragments arranged by size: 

                1357     845     385     195      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     142     148     430     616     850    1015    1300 
   Size:         55      87       6     282     186     234     165     285

Cuts at:   1300    1324    1613    2844 
   Size:         24     289    1231

  Fragments arranged by size: 

                1231     289     285     282     234     186     165      87
                  55      24       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     391    1099    1433    1747    2844 
   Size:        391     708     334     314    1097

  Fragments arranged by size: 

                1097     708     391     334     314
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1013    1024    1786    1795    2844 
   Size:       1013      11     762       9    1049

  Fragments arranged by size: 

                1049    1013     762      11       9
 
BsaAI yAC'GTr

Cuts at:      0     309    1559    2088    2844 
   Size:        309    1250     529     756

  Fragments arranged by size: 

                1250     756     529     309
 
BsaBI GATnn'nnATC

Cuts at:      0     550    1543    2844 
   Size:        550     993    1301

  Fragments arranged by size: 

                1301     993     550
 
BsaHI Gr'CG_yC

Cuts at:      0    1231    2673    2717    2844 
   Size:       1231    1442      44     127

  Fragments arranged by size: 

                1442    1231     127      44
 
BsaWI w'CCGG_w

Cuts at:      0     494    1574    2079    2327    2844 
   Size:        494    1080     505     248     517

  Fragments arranged by size: 

                1080     517     505     494     248
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     403     433    2074    2104    2844 
   Size:        403      30    1641      30     740

  Fragments arranged by size: 

                1641     740     403      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     246     894    1897    2491    2494    2749    2844 
   Size:        246     648    1003     594       3     255      95

  Fragments arranged by size: 

                1003     648     594     255     246      95       3
 
BseYI C'CCAG_C

Cuts at:      0      72     515     807    1107    1878    2193    2589    2651 
   Size:         72     443     292     300     771     315     396      62

Cuts at:   2651    2844 
   Size:        193

  Fragments arranged by size: 

                 771     443     396     315     300     292     193      72
                  62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     283    1417    1842    2116    2844 
   Size:        262      21    1134     425     274     728

  Fragments arranged by size: 

                1134     728     425     274     262      21
 
BsiEI CG_ry'CG

Cuts at:      0    2114    2537    2709    2844 
   Size:       2114     423     172     135

  Fragments arranged by size: 

                2114     423     172     135
 
BsiHKAI G_wGCw'C

Cuts at:      0     695     893    1717    1753    1907    2087    2844 
   Size:        695     198     824      36     154     180     757

  Fragments arranged by size: 

                 824     757     695     198     180     154      36
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1973    2844 
   Size:       1973     871
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     256     695     893     938    1296    1347    1603 
   Size:        232      24     439     198      45     358      51     256

Cuts at:   1603    1717    1753    1907    2087    2162    2696    2767    2844 
   Size:        114      36     154     180      75     534      71      77

  Fragments arranged by size: 

                 534     439     358     256     232     198     180     154
                 114      77      75      71      51      45      36      24
            
 
BspEI T'CCGG_A

Cuts at:      0    1574    2844 
   Size:       1574    1270
 
BspHI T'CATG_A

Cuts at:      0    2552    2844 
   Size:       2552     292
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     200     281     382    2844 
   Size:        200      81     101    2462

  Fragments arranged by size: 

                2462     200     101      81
 
BsrBI CCG'CTC

Cuts at:      0     132     138     834     981    2583    2844 
   Size:        132       6     696     147    1602     261

  Fragments arranged by size: 

                1602     696     261     147     132       6
 
BsrDI GCAATG_nn'

Cuts at:      0     483    2844 
   Size:        483    2361
 
BsrFI r'CCGG_y

Cuts at:      0     586    2113    2844 
   Size:        586    1527     731

  Fragments arranged by size: 

                1527     731     586
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297     525     965    1395    2844 
   Size:        297     228     440     430    1449

  Fragments arranged by size: 

                1449     440     430     297     228
 
BstEII G'GTnAC_C

Cuts at:      0    1437    2844 
   Size:       1437    1407
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     213     355    2844 
   Size:        213     142    2489

  Fragments arranged by size: 

                2489     213     142
 
BstYI r'GATC_y

Cuts at:      0    1633    1722    2593    2844 
   Size:       1633      89     871     251

  Fragments arranged by size: 

                1633     871     251      89
 
Bsu36I CC'TnA_GG

Cuts at:      0    2369    2844 
   Size:       2369     475
 
BtgI C'CryG_G

Cuts at:      0    1039    1595    1649    2795    2844 
   Size:       1039     556      54    1146      49

  Fragments arranged by size: 

                1146    1039     556      54      49
 
BtsI GCAGTG_nn'

Cuts at:      0    1769    2030    2844 
   Size:       1769     261     814

  Fragments arranged by size: 

                1769     814     261
 
ClaI AT'CG_AT

Cuts at:      0    1547    2844 
   Size:       1547    1297
 
DraIII CAC_nnn'GTG

Cuts at:      0     312     699     965    2844 
   Size:        312     387     266    1879

  Fragments arranged by size: 

                1879     387     312     266
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2385    2497    2844 
   Size:       2385     112     347

  Fragments arranged by size: 

                2385     347     112
 
EaeI y'GGCC_r

Cuts at:      0      80     329     380     422     588     701     929    1146 
   Size:         80     249      51      42     166     113     228     217

Cuts at:   1146    2111    2534    2706    2831    2844 
   Size:        965     423     172     125      13

  Fragments arranged by size: 

                 965     423     249     228     217     172     166     125
                 113      80      51      42      13
 
EagI C'GGCC_G

Cuts at:      0    2111    2534    2706    2844 
   Size:       2111     423     172     138

  Fragments arranged by size: 

                2111     423     172     138
 
EarI CTCTTCn'nnn_

Cuts at:      0     659     881    1705    2065    2844 
   Size:        659     222     824     360     779

  Fragments arranged by size: 

                 824     779     659     360     222
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     466     559    2169    2844 
   Size:         19     447      93    1610     675

  Fragments arranged by size: 

                1610     675     447      93      19
 
EcoICRI GAG'CTC

Cuts at:      0     891    1715    1751    2844 
   Size:        891     824      36    1093

  Fragments arranged by size: 

                1093     891     824      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     142     148     187     193     430     454     616 
   Size:         55      87       6      39       6     237      24     162

Cuts at:    616     850     900    1015    1300    1324    1378    1613    1975 
   Size:        234      50     115     285      24      54     235     362

Cuts at:   1975    2260    2622    2844 
   Size:        285     362     222

  Fragments arranged by size: 

                 362     362     285     285     237     235     234     222
                 162     115      87      55      54      50      39      24
                  24       6       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2302    2637    2844 
   Size:       2302     335     207

  Fragments arranged by size: 

                2302     335     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0     229     747     935     989    1066    1073    1116    1335 
   Size:        229     518     188      54      77       7      43     219

Cuts at:   1335    1810    2121    2235    2348    2751    2844 
   Size:        475     311     114     113     403      93

  Fragments arranged by size: 

                 518     475     403     311     229     219     188     114
                 113      93      77      54      43       7
 
EcoRI G'AATT_C

Cuts at:      0    1915    2844 
   Size:       1915     929
 
FseI GG_CCGG'CC

Cuts at:      0     590    2844 
   Size:        590    2254
 
FspI TGC'GCA

Cuts at:      0     658    2844 
   Size:        658    2186
 
HaeII r_GCGC'y

Cuts at:      0     580     740    2676    2844 
   Size:        580     160    1936     168

  Fragments arranged by size: 

                1936     580     168     160
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2137    2169    2844 
   Size:       2137      32     675

  Fragments arranged by size: 

                2137     675      32
 
HincII GTy'rAC

Cuts at:      0    2502    2844 
   Size:       2502     342
 
KasI G'GCGC_C

Cuts at:      0    2672    2844 
   Size:       2672     172
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     821    1343    1503    2225    2844 
   Size:        821     522     160     722     619

  Fragments arranged by size: 

                 821     722     619     522     160
 
MscI TGG'CCA

Cuts at:      0     424    2844 
   Size:        424    2420
 
MslI CAynn'nnrTG

Cuts at:      0     559    1219    1561    1663    2844 
   Size:        559     660     342     102    1181

  Fragments arranged by size: 

                1181     660     559     342     102
 
MspA1I CmG'CkG

Cuts at:      0      53      72     120     288     594     910    1332    1401 
   Size:         53      19      48     168     306     316     422      69

Cuts at:   1401    2797    2844 
   Size:       1396      47

  Fragments arranged by size: 

                1396     422     316     306     168      69      53      48
                  47      19
 
NaeI GCC'GGC

Cuts at:      0     588    2844 
   Size:        588    2256
 
NarI GG'CG_CC

Cuts at:      0    2673    2844 
   Size:       2673     171
 
NgoMIV G'CCGG_C

Cuts at:      0     586    2844 
   Size:        586    2258
 
NspI r_CATG'y

Cuts at:      0     564    2844 
   Size:        564    2280
 
PasI CC'CwG_GG

Cuts at:      0    1416    1618    2464    2844 
   Size:       1416     202     846     380

  Fragments arranged by size: 

                1416     846     380     202
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     807    1040    1259    2844 
   Size:        807     233     219    1585

  Fragments arranged by size: 

                1585     807     233     219
 
PfoI T'CCnGG_A

Cuts at:      0    1629    1760    2258    2813    2844 
   Size:       1629     131     498     555      31

  Fragments arranged by size: 

                1629     555     498     131      31
 
PmlI CAC'GTG

Cuts at:      0     309    1559    2088    2844 
   Size:        309    1250     529     756

  Fragments arranged by size: 

                1250     756     529     309
 
PpuMI rG'GwC_Cy

Cuts at:      0     989    1066    1073    2121    2235    2348    2844 
   Size:        989      77       7    1048     114     113     496

  Fragments arranged by size: 

                1048     989     496     114     113      77       7
 
PspOMI G'GGCC_C

Cuts at:      0     228     934    2844 
   Size:        228     706    1910

  Fragments arranged by size: 

                1910     706     228
 
PstI C_TGCA'G

Cuts at:      0     293     584    1018    2844 
   Size:        293     291     434    1826

  Fragments arranged by size: 

                1826     434     293     291
 
PvuII CAG'CTG

Cuts at:      0      53      72     120     288     594    1401    2844 
   Size:         53      19      48     168     306     807    1443

  Fragments arranged by size: 

                1443     807     306     168      53      48      19
 
RsrII CG'GwC_CG

Cuts at:      0     753    2844 
   Size:        753    2091
 
SacI G_AGCT'C

Cuts at:      0     893    1717    1753    2844 
   Size:        893     824      36    1091

  Fragments arranged by size: 

                1091     893     824      36
 
SacII CC_GC'GG

Cuts at:      0    2798    2844 
   Size:       2798      46
 
SanDI GG'GwC_CC

Cuts at:      0     989    1073    2348    2844 
   Size:        989      84    1275     496

  Fragments arranged by size: 

                1275     989     496      84
 
SapI GCTCTTCn'nnn_

Cuts at:      0     659     881    1705    2844 
   Size:        659     222     824    1139

  Fragments arranged by size: 

                1139     824     659     222
 
ScaI AGT'ACT

Cuts at:      0    2107    2844 
   Size:       2107     737
 
SfcI C'TryA_G

Cuts at:      0     289     471     580     954    1014    2200    2229    2844 
   Size:        289     182     109     374      60    1186      29     615

  Fragments arranged by size: 

                1186     615     374     289     182     109      60      29
            
 
SfoI GGC'GCC

Cuts at:      0    2674    2844 
   Size:       2674     170
 
SmaI CCC'GGG

Cuts at:      0     105    1114    2844 
   Size:        105    1009    1730

  Fragments arranged by size: 

                1730    1009     105
 
SmlI C'TyrA_G

Cuts at:      0    1201    2844 
   Size:       1201    1643
 
SphI G_CATG'C

Cuts at:      0     564    2844 
   Size:        564    2280
 
StyI C'CwwG_G

Cuts at:      0     488     624     743     866    2844 
   Size:        488     136     119     123    1978

  Fragments arranged by size: 

                1978     488     136     123     119
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     349     637    1551    2410    2844 
   Size:        349     288     914     859     434

  Fragments arranged by size: 

                 914     859     434     349     288
 
TatI w'GTAC_w

Cuts at:      0    2105    2844 
   Size:       2105     739
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1230    1262    1638    1670    2074    2106    2408    2440 
   Size:       1230      32     376      32     404      32     302      32

Cuts at:   2440    2844 
   Size:        404

  Fragments arranged by size: 

                1230     404     404     376     302      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210     804    2844 
   Size:        210     594    2040

  Fragments arranged by size: 

                2040     594     210
 
XmaI C'CCGG_G

Cuts at:      0     103    1112    2844 
   Size:        103    1009    1732

  Fragments arranged by size: 

                1732    1009     103
 
XmnI GAAnn'nnTTC

Cuts at:      0    1495    1519    2059    2844 
   Size:       1495      24     540     785

  Fragments arranged by size: 

                1495     785     540      24
 
ZraI GAC'GTC

Cuts at:      0    1232    2718    2844 
   Size:       1232    1486     126

  Fragments arranged by size: 

                1486    1232     126
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI    AflII   AflIII     AleI
    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FseI     FspI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     ScaI     SfcI     SfoI     SmaI
     SmlI     SphI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AgeI     AhdI     AlfI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI    BglII
     BmtI    BpuEI    BsiWI     BsmI    BsrGI   BssHII    BssSI    BstBI
  BstZ17I    BtgZI    CspCI     DraI    EcoRV     FalI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI     SalI     SbfI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI
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