Restriction map for pF1KB9986
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# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BcgI        BclI        BcnI        BlnI        BpmI        
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      Bst1107I    
BstEII      BstPI       BstXI       BstZ17I     Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SapI        
SbfI        SexAI       SfcI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII        1100  GACGTC               GACGT^C
AccII        1787  CGCG                 CG^CG
AcyI         1100  GACGTC               G[GA]^CG[CT]C
AflII        1020  CTTAAG               C^TTAAG
AflIII       1321  ACATGT               A^C[GA][CT]GT
AocI          109  CCTGAGG              CC^T[ACGT]AGG
AspHI         847  GAGCAC               G[AT]GC[AT]^C
AvaII         585  GGACC                G^G[AT]CC
BbvCI        1377  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1284  GTATCC               GTATCC(6/5)
BfrI         1020  CTTAAG               C^TTAAG
BglI         1554  GCCAAAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          62  AGATCT               A^GATCT
BlpI          597  GCTAAGC              GC^T[ACGT]AGC
BmyI          847  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      597  GCTAAGC              GC^T[ACGT]AGC
BsaAI        1123  TACGTG               [CT]AC^GT[GA]
BsaHI        1100  GACGTC               G[GA]^CG[CT]C
BsaJI        1458  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       847  GAGCAC               G[AT]GC[AT]^C
BsmI         1865  GCATTC               GAATGC(1/-1)
Bsp1286I      847  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I     1318  TGTACA               T^GTACA
BspLU11I     1321  ACATGT               A^CATGT
BspMI        1211  ACCTGC               ACCTGC(4/8)
BsrDI         404  CATTGC               GCAATG(2/0)
BsrGI        1318  TGTACA               T^GTACA
BssSI        1936  CTCGTG               CACGAG(-5/-1)
BstAPI       1993  GCAAATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         130  TTCGAA               TT^CGAA
BstUI        1787  CGCG                 CG^CG
BstYI          62  AGATCT               [GA]^GATC[CT]
Bsu36I        109  CCTGAGG              CC^T[ACGT]AGG
CelII         597  GCTAAGC              GC^T[ACGT]AGC
CfoI         1156  GCGC                 GCG^C
DraII         368  AGGCCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        109  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      368  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         258  GAATTC               G^AATTC
EcoT14I      1458  CCTTGG               C^C[AT][AT]GG
EcoT22I      1358  ATGCAT               ATGCA^T
HhaI         1156  GCGC                 GCG^C
Hin1I        1100  GACGTC               G[GA]^CG[CT]C
HinP1I       1156  GCGC                 G^CGC
MflI           62  AGATCT               [GA]^GATC[CT]
MvnI         1787  CGCG                 CG^CG
NlaIV         585  GGACCC               GG[ACGT]^[ACGT]CC
NsiI         1358  ATGCAT               ATGCA^T
NspV          130  TTCGAA               TT^CGAA
PflMI        1728  CCATGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI         1295  GTCGAC               G^TCGAC
SfuI          130  TTCGAA               TT^CGAA
SspBI        1318  TGTACA               T^GTACA
StyI         1458  CCTTGG               C^C[AT][AT]GG
SwaI         1772  ATTTAAAT             ATTT^AAAT
Van91I       1728  CCATGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          62  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1103  GTCTAC               GT^[AC][GT]AC
             1295  GTCGAC              
AciI         1786  CCGC                 CCGC(-3/-1)
              275  GCGG                
BsgI         1805  CTGCAC               GTGCAG(16/14)
             1906  CTGCAC              
BsiYI         852  CCTGACTACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1728  CCATGAGGTGG         
BslI          852  CCTGACTACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1728  CCATGAGGTGG         
BspHI         339  TCATGA               T^CATGA
              948  TCATGA              
BssKI        1239  CCTGG                ^CC[ACGT]GG
             1491  CCTGG               
BstNI        1239  CCTGG                CC^[AT]GG
             1491  CCTGG               
DraI          898  TTTAAA               TTT^AAA
             1773  TTTAAA              
EarI         1269  CTCTTC               CTCTTC(1/4)
             1274  CTCTTC              
EcoRII       1239  CCTGG                ^CC[AT]GG
             1491  CCTGG               
HaeIII        369  GGCC                 GG^CC
             1494  GGCC                
HincII        713  GTCAAC               GT[CT]^[GA]AC
             1295  GTCGAC              
HindII        713  GTCAAC               GT[CT]^[GA]AC
             1295  GTCGAC              
HphI          515  TCACC                GGTGA(8/7)
             1265  TCACC               
Ksp632I      1269  CTCTTC               CTCTTC(1/4)
             1274  CTCTTC              
MslI          718  CATCTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1771  CATTTAAATG          
MspA1I       1372  CAGCTG               C[AC]G^C[GT]G
             1973  CAGCTG              
MvaI         1239  CCTGG                CC^[AT]GG
             1491  CCTGG               
NspI         1215  GCATGT               [GA]CATG^[CT]
             1321  ACATGT              
PvuII        1372  CAGCTG               CAG^CTG
             1973  CAGCTG              
RcaI          339  TCATGA               T^CATGA
              948  TCATGA              
ScaI          684  AGTACT               AGT^ACT
             1650  AGTACT              
ScrFI        1239  CCTGG                CC^[ACGT]GG
             1491  CCTGG               
TspRI        1746  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1844  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwNI       ApoI        
Asp700I     BbsI        BbvI        BfaI        BpuAI       
BseRI       BsmAI       BsrI        BstF5I      Cac8I       
Cfr13I      DdeI        DpnII       Fnu4HI      FokI        
HindIII     HinfI       ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MwoI        NdeI        NdeII       NlaIII      RsaI        
Sau3AI      Sau96I      SfaNI       SmlI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TthHB8I     XmnI        
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