Restriction map for pF1KB9984
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoT22I     FbaI        FseI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SwaI        Tru9I       Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          301  GTCTAC               GT^[AC][GT]AC
AccII        1088  CGCG                 CG^CG
AhdI          450  GACACTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         84  GTGCAC               G^TGCAC
AocI         2124  CCTCAGG              CC^T[ACGT]AGG
ApaI           35  GGGCCC               GGGCC^C
ApaLI          84  GTGCAC               G^TGCAC
AspEI         450  GACACTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI           44  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1124  TGCGCA               TGC^GCA
BbsI           10  GTCTTC               GAAGAC(2/6)
BpuAI          10  GTCTTC               GAAGAC(2/6)
BsaWI        1655  TCCGGT               [AT]^CCGG[AT]
BsgI          539  GTGCAG               GTGCAG(16/14)
BsmI         1671  GAATGC               GAATGC(1/-1)
Bsp1407I     1256  TGTACA               T^GTACA
BspMI        1168  ACCTGC               ACCTGC(4/8)
BsrGI        1256  TGTACA               T^GTACA
BstUI        1088  CGCG                 CG^CG
Bsu36I       2124  CCTCAGG              CC^T[ACGT]AGG
Eam1105I      450  GACACTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2124  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2119  GAATTC               G^AATTC
EcoRV        1361  GATATC               GAT^ATC
FspI         1124  TGCGCA               TGC^GCA
MvnI         1088  CGCG                 CG^CG
NdeI         2033  CATATG               CA^TATG
PflFI          44  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         1485  GCTCTTC              GCTCTTC(1/4)
SmaI          803  CCCGGG               CCC^GGG
SspBI        1256  TGTACA               T^GTACA
Tth111I        44  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          460  CCATTCTTGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         803  CCCGGG               C^CCGGG
XmaI          803  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwI         1330  GATCC                GGATC(4/5)
             1930  GGATC               
AlwNI        1117  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1891  CAGGTGCTG           
BalI          506  TGGCCA               TGG^CCA
             2009  TGGCCA              
BciVI          91  GTATCC               GTATCC(6/5)
              194  GTATCC              
BglII         948  AGATCT               A^GATCT
             1558  AGATCT              
BpmI          668  CTGGAG               CTGGAG(16/14)
             1676  CTGGAG              
BstAPI       1116  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1765  GCAGACTTTGC         
DraIII        830  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1998  CACTGAGTG           
EarI         1486  CTCTTC               CTCTTC(1/4)
              140  GAAGAG              
EcoNI        1351  CCTGTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1884  CCTGAAACAGG         
FauI          580  GCGGG                CCCGC(4/6)
             1089  GCGGG               
Ksp632I      1486  CTCTTC               CTCTTC(1/4)
              140  GAAGAG              
MaeII         674  ACGT                 A^CGT
             1682  ACGT                
MluNI         506  TGGCCA               TGG^CCA
             2009  TGGCCA              
MscI          506  TGGCCA               TGG^CCA
             2009  TGGCCA              
MslI          331  CACTATTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1440  CACCGCCGTG          
PpuMI         707  AGGACCT              [GA]G^G[AT]CC[CT]
              806  GGGTCCC             
PstI         1262  CTGCAG               CTGCA^G
             1457  CTGCAG              
SmlI         1050  CTTGAG               C^T[CT][GA]AG
             1149  CTTGAG              
SspI         1520  AATATT               AAT^ATT
             2042  AATATT              
StuI          261  AGGCCT               AGG^CCT
              411  AGGCCT              
TaqI          982  TCGA                 T^CGA
             1787  TCGA                
TthHB8I       982  TCGA                 T^CGA
             1787  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BglI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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