Restriction map for pF1KB9984
 (Linear) (Six-base) MAPSORT of: KIBB9984.seq  Check: 3798  from: 1  to: 2133



KIBB9984.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:      0     302    2133 
   Size:        302    1831
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     612    2133 
   Size:        415     197    1521

  Fragments arranged by size: 

                1521     415     197
 
AhdI GACnn_n'nnGTC

Cuts at:      0     455    2133 
   Size:        455    1678
 
AleI CACnn'nnGTG

Cuts at:      0    1444    2133 
   Size:       1444     689
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1291    1323    2133 
   Size:       1291      32     810

  Fragments arranged by size: 

                1291     810      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1122    1896    2133 
   Size:       1122     774     237

  Fragments arranged by size: 

                1122     774     237
 
ApaI G_GGCC'C

Cuts at:      0      39    2133 
   Size:         39    2094
 
ApaLI G'TGCA_C

Cuts at:      0      84    2133 
   Size:         84    2049
 
ApoI r'AATT_y

Cuts at:      0     913    1510    1915    2119    2133 
   Size:        913     597     405     204      14

  Fragments arranged by size: 

                 913     597     405     204      14
 
AvaI C'yCGr_G

Cuts at:      0      31     100     803     993    1554    2133 
   Size:         31      69     703     190     561     579

  Fragments arranged by size: 

                 703     579     561     190      69      31
 
BanI G'GyrC_C

Cuts at:      0     640     987    1281    1658    2133 
   Size:        640     347     294     377     475

  Fragments arranged by size: 

                 640     475     377     347     294
 
BanII G_rGCy'C

Cuts at:      0      39     491    1504    2133 
   Size:         39     452    1013     629

  Fragments arranged by size: 

                1013     629     452      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       3    2133 
   Size:          3    2130
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     102     205    2133 
   Size:        102     103    1928

  Fragments arranged by size: 

                1928     103     102
 
BglI GCCn_nnn'nGGC

Cuts at:      0     410     826    1790    2133 
   Size:        410     416     964     343

  Fragments arranged by size: 

                 964     416     410     343
 
BglII A'GATC_T

Cuts at:      0     948    1558    2133 
   Size:        948     610     575

  Fragments arranged by size: 

                 948     610     575
 
Bme1580I G_kGCm'C

Cuts at:      0      39      88     992    1542    1616    2133 
   Size:         39      49     904     550      74     517

  Fragments arranged by size: 

                 904     550     517      74      49      39
 
BmgBI CAC'GTC

Cuts at:      0     675    2133 
   Size:        675    1458
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1655    2133 
   Size:       1655     478
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    1697    2133 
   Size:        689    1008     436

  Fragments arranged by size: 

                1008     689     436
 
Bpu10I CC'TnA_GC

Cuts at:      0     485    1748    2133 
   Size:        485    1263     385

  Fragments arranged by size: 

                1263     485     385
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1071    1170    2133 
   Size:       1071      99     963

  Fragments arranged by size: 

                1071     963      99
 
BsaWI w'CCGG_w

Cuts at:      0    1655    2133 
   Size:       1655     478
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      57      87    1006    1036    1238    1268    2133 
   Size:         57      30     919      30     202      30     865

  Fragments arranged by size: 

                 919     865     202      57      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     600    1078    1164    2133 
   Size:        600     478      86     969

  Fragments arranged by size: 

                 969     600     478      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     560    2133 
   Size:        560    1573
 
BsiHKAI G_wGCw'C

Cuts at:      0      88     697    1575    2092    2133 
   Size:         88     609     878     517      41

  Fragments arranged by size: 

                 878     609     517      88      41
 
BsmI GAATG_Cn'

Cuts at:      0    1677    2133 
   Size:       1677     456
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     653     665    1673    2133 
   Size:        653      12    1008     460

  Fragments arranged by size: 

                1008     653     460      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      39      88     491     697     992    1504    1542    1575 
   Size:         39      49     403     206     295     512      38      33

Cuts at:   1575    1616    2092    2133 
   Size:         41     476      41

  Fragments arranged by size: 

                 512     476     403     295     206      49      41      41
                  39      38      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1177    2133 
   Size:       1177     956
 
BsrDI GCAATG_nn'

Cuts at:      0     203    1199    1629    2133 
   Size:        203     996     430     504

  Fragments arranged by size: 

                 996     504     430     203
 
BsrGI T'GTAC_A

Cuts at:      0    1256    2133 
   Size:       1256     877
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1122    1771    2133 
   Size:       1122     649     362

  Fragments arranged by size: 

                1122     649     362
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     925    1144    1239    2133 
   Size:        925     219      95     894

  Fragments arranged by size: 

                 925     894     219      95
 
BstYI r'GATC_y

Cuts at:      0     948    1558    1930    2133 
   Size:        948     610     372     203

  Fragments arranged by size: 

                 948     610     372     203
 
Bsu36I CC'TnA_GG

Cuts at:      0    2125    2133 
   Size:       2125       8
 
BtgI C'CryG_G

Cuts at:      0     341    1445    1475    1835    1853    2019    2133 
   Size:        341    1104      30     360      18     166     114

  Fragments arranged by size: 

                1104     360     341     166     114      30      18
 
BtsI GCAGTG_nn'

Cuts at:      0    1034    1167    1259    1326    2133 
   Size:       1034     133      92      67     807

  Fragments arranged by size: 

                1034     807     133      92      67
 
DraIII CAC_nnn'GTG

Cuts at:      0     835    2003    2133 
   Size:        835    1168     130

  Fragments arranged by size: 

                1168     835     130
 
EaeI y'GGCC_r

Cuts at:      0      57     506    2009    2133 
   Size:         57     449    1503     124

  Fragments arranged by size: 

                1503     449     124      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     135    1492    2133 
   Size:        135    1357     641

  Fragments arranged by size: 

                1357     641     135
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     310     862    2133 
   Size:        310     552    1271

  Fragments arranged by size: 

                1271     552     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     612     689    1697    2133 
   Size:        415     197      77    1008     436

  Fragments arranged by size: 

                1008     436     415     197      77
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1355    1888    2133 
   Size:       1355     533     245

  Fragments arranged by size: 

                1355     533     245
 
EcoO109I rG'GnC_Cy

Cuts at:      0      35      36     708     807    2133 
   Size:         35       1     672      99    1326

  Fragments arranged by size: 

                1326     672      99      35       1
 
EcoRI G'AATT_C

Cuts at:      0    2119    2133 
   Size:       2119      14
 
EcoRV GAT'ATC

Cuts at:      0    1363    2133 
   Size:       1363     770
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1381    1971    2133 
   Size:       1381     590     162

  Fragments arranged by size: 

                1381     590     162
 
FspI TGC'GCA

Cuts at:      0    1126    2133 
   Size:       1126    1007
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     422     441     454     473     697     729    1543    1548 
   Size:        422      19      13      19     224      32     814       5

Cuts at:   1548    1550    1575    1580    1582    2133 
   Size:          2      25       5       2     551

  Fragments arranged by size: 

                 814     551     422     224      32      25      19      19
                  13       5       5       2       2
 
MscI TGG'CCA

Cuts at:      0     508    2011    2133 
   Size:        508    1503     122

  Fragments arranged by size: 

                1503     508     122
 
MslI CAynn'nnrTG

Cuts at:      0     335    1444    2133 
   Size:        335    1109     689

  Fragments arranged by size: 

                1109     689     335
 
MspA1I CmG'CkG

Cuts at:      0     164     375     427     502     527    2133 
   Size:        164     211      52      75      25    1606

  Fragments arranged by size: 

                1606     211     164      75      52      25
 
NcoI C'CATG_G

Cuts at:      0    1475    1835    2019    2133 
   Size:       1475     360     184     114

  Fragments arranged by size: 

                1475     360     184     114
 
NdeI CA'TA_TG

Cuts at:      0    2034    2133 
   Size:       2034      99
 
PasI CC'CwG_GG

Cuts at:      0    1543    2133 
   Size:       1543     590
 
PfoI T'CCnGG_A

Cuts at:      0    2105    2133 
   Size:       2105      28
 
PpuMI rG'GwC_Cy

Cuts at:      0     708     807    2133 
   Size:        708      99    1326

  Fragments arranged by size: 

                1326     708      99
 
PspOMI G'GGCC_C

Cuts at:      0      35    2133 
   Size:         35    2098
 
PstI C_TGCA'G

Cuts at:      0    1266    1461    2133 
   Size:       1266     195     672

  Fragments arranged by size: 

                1266     672     195
 
PvuII CAG'CTG

Cuts at:      0     164     375     502     527    2133 
   Size:        164     211     127      25    1606

  Fragments arranged by size: 

                1606     211     164     127      25
 
SanDI GG'GwC_CC

Cuts at:      0     807    2133 
   Size:        807    1326
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1492    2133 
   Size:       1492     641
 
SfcI C'TryA_G

Cuts at:      0     287     443    1262    1457    2133 
   Size:        287     156     819     195     676

  Fragments arranged by size: 

                 819     676     287     195     156
 
SmaI CCC'GGG

Cuts at:      0     805    2133 
   Size:        805    1328
 
SmlI C'TyrA_G

Cuts at:      0    1050    1149    2133 
   Size:       1050      99     984

  Fragments arranged by size: 

                1050     984      99
 
SspI AAT'ATT

Cuts at:      0    1522    2044    2133 
   Size:       1522     522      89

  Fragments arranged by size: 

                1522     522      89
 
StuI AGG'CCT

Cuts at:      0     263     413    2133 
   Size:        263     150    1720

  Fragments arranged by size: 

                1720     263     150
 
StyI C'CwwG_G

Cuts at:      0    1017    1475    1835    2019    2133 
   Size:       1017     458     360     184     114

  Fragments arranged by size: 

                1017     458     360     184     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     325    2133 
   Size:        325    1808
 
TatI w'GTAC_w

Cuts at:      0    1256    2133 
   Size:       1256     877
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      57      89     899     931    1238    1270    2133 
   Size:         57      32     810      32     307      32     863

  Fragments arranged by size: 

                 863     810     307      57      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      47    2133 
   Size:         47    2086
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     467    2133 
   Size:        467    1666
 
XmaI C'CCGG_G

Cuts at:      0     803    2133 
   Size:        803    1330
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbsI    BciVI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI
    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    Hin4I     MscI     MslI   MspA1I     NcoI     NdeI
     PasI     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI
     SfcI     SmaI     SmlI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI     BclI    BfrBI     BlpI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BseYI    BsiEI    BsiWI    BspEI    BspHI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI     FseI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SwaI     XbaI     XhoI     XmnI     ZraI
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