Restriction map for
pF1KB8503
(Linear) (Six-base) MAPSORT of: KIBB8503.seq Check: 4108 from: 1 to: 2811
KIBB8503.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 1013 2632 2811
Size: 1013 1619 179
Fragments arranged by size:
1619 1013 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1376 1665 1856 1910 2024 2044 2811
Size: 1082 294 289 191 54 114 20 767
Fragments arranged by size:
1082 767 294 289 191 114 54 20
AflIII A'CryG_T
Cuts at: 0 2371 2811
Size: 2371 440
AleI CACnn'nnGTG
Cuts at: 0 2690 2811
Size: 2690 121
AlwNI CAG_nnn'CTG
Cuts at: 0 1564 2811
Size: 1564 1247
ApaI G_GGCC'C
Cuts at: 0 2498 2811
Size: 2498 313
ApoI r'AATT_y
Cuts at: 0 79 88 208 396 688 819 970 1438
Size: 79 9 120 188 292 131 151 468
Cuts at: 1438 1961 2811
Size: 523 850
Fragments arranged by size:
850 523 468 292 188 151 131 120
79 9
AseI AT'TA_AT
Cuts at: 0 800 2811
Size: 800 2011
BamHI G'GATC_C
Cuts at: 0 2544 2811
Size: 2544 267
BanII G_rGCy'C
Cuts at: 0 2285 2498 2811
Size: 2285 213 313
Fragments arranged by size:
2285 313 213
BbsI GAAGACnn'nnnn_
Cuts at: 0 1382 2650 2811
Size: 1382 1268 161
Fragments arranged by size:
1382 1268 161
BbvCI CC'TCA_GC
Cuts at: 0 683 2811
Size: 683 2128
BciVI GTATCCnnnnn_n'
Cuts at: 0 719 2811
Size: 719 2092
BclI T'GATC_A
Cuts at: 0 2382 2811
Size: 2382 429
BfrBI ATG'CAT
Cuts at: 0 1106 2614 2742 2811
Size: 1106 1508 128 69
Fragments arranged by size:
1508 1106 128 69
Bme1580I G_kGCm'C
Cuts at: 0 2252 2498 2811
Size: 2252 246 313
Fragments arranged by size:
2252 313 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 556 1826 1975 2442 2811
Size: 437 119 1270 149 467 369
Fragments arranged by size:
1270 467 437 369 149 119
Bpu10I CC'TnA_GC
Cuts at: 0 683 2811
Size: 683 2128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 2160 2577 2777 2811
Size: 1340 820 417 200 34
Fragments arranged by size:
1340 820 417 200 34
BsaI GGTCTCn'nnnn_
Cuts at: 0 1760 2811
Size: 1760 1051
BsaWI w'CCGG_w
Cuts at: 0 2547 2811
Size: 2547 264
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2415 2445 2811
Size: 2415 30 366
Fragments arranged by size:
2415 366 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1486 2811
Size: 1486 1325
BseYI C'CCAG_C
Cuts at: 0 2763 2811
Size: 2763 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 2811
Size: 770 2041
BsiHKAI G_wGCw'C
Cuts at: 0 1098 1400 2687 2811
Size: 1098 302 1287 124
Fragments arranged by size:
1287 1098 302 124
BsmI GAATG_Cn'
Cuts at: 0 1722 2811
Size: 1722 1089
Bsp1286I G_dGCh'C
Cuts at: 0 1098 1400 2252 2285 2498 2687 2811
Size: 1098 302 852 33 213 189 124
Fragments arranged by size:
1098 852 302 213 189 124 33
BspEI T'CCGG_A
Cuts at: 0 2547 2811
Size: 2547 264
BspHI T'CATG_A
Cuts at: 0 204 2811
Size: 204 2607
BsrDI GCAATG_nn'
Cuts at: 0 519 1278 1653 2811
Size: 519 759 375 1158
Fragments arranged by size:
1158 759 519 375
BsrGI T'GTAC_A
Cuts at: 0 2374 2811
Size: 2374 437
BstAPI GCAn_nnn'nTGC
Cuts at: 0 700 2811
Size: 700 2111
BstBI TT'CG_AA
Cuts at: 0 18 1967 2811
Size: 18 1949 844
Fragments arranged by size:
1949 844 18
BstYI r'GATC_y
Cuts at: 0 2544 2811
Size: 2544 267
Bsu36I CC'TnA_GG
Cuts at: 0 581 2811
Size: 581 2230
BtgI C'CryG_G
Cuts at: 0 2498 2811
Size: 2498 313
BtsI GCAGTG_nn'
Cuts at: 0 275 566 955 2436 2811
Size: 275 291 389 1481 375
Fragments arranged by size:
1481 389 375 291 275
DraI TTT'AAA
Cuts at: 0 401 758 2811
Size: 401 357 2053
Fragments arranged by size:
2053 401 357
EaeI y'GGCC_r
Cuts at: 0 1902 2811
Size: 1902 909
EarI CTCTTCn'nnn_
Cuts at: 0 33 164 1379 2000 2221 2477 2675 2811
Size: 33 131 1215 621 221 256 198 136
Fragments arranged by size:
1215 621 256 221 198 136 131 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 556 1082 1376 1665 1826 1856 1910
Size: 437 119 526 294 289 161 30 54
Cuts at: 1910 1975 2024 2044 2442 2811
Size: 65 49 20 398 369
Fragments arranged by size:
526 437 398 369 294 289 161 119
65 54 49 30 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 299 2811
Size: 299 2512
EcoRI G'AATT_C
Cuts at: 0 208 819 2811
Size: 208 611 1992
Fragments arranged by size:
1992 611 208
EcoRV GAT'ATC
Cuts at: 0 1278 2121 2811
Size: 1278 843 690
Fragments arranged by size:
1278 843 690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1087 2811
Size: 1087 1724
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 1504 1536 2497 2529 2811
Size: 1300 32 172 32 961 32 282
Fragments arranged by size:
1300 961 282 172 32 32 32
HincII GTy'rAC
Cuts at: 0 2564 2774 2811
Size: 2564 210 37
Fragments arranged by size:
2564 210 37
HindIII A'AGCT_T
Cuts at: 0 237 849 2176 2811
Size: 237 612 1327 635
Fragments arranged by size:
1327 635 612 237
HpaI GTT'AAC
Cuts at: 0 2564 2774 2811
Size: 2564 210 37
Fragments arranged by size:
2564 210 37
MfeI C'AATT_G
Cuts at: 0 1534 2811
Size: 1534 1277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 545 976 2811
Size: 411 134 431 1835
Fragments arranged by size:
1835 431 411 134
MscI TGG'CCA
Cuts at: 0 1904 2811
Size: 1904 907
MslI CAynn'nnrTG
Cuts at: 0 19 1412 2690 2811
Size: 19 1393 1278 121
Fragments arranged by size:
1393 1278 121 19
MspA1I CmG'CkG
Cuts at: 0 1021 1690 2811
Size: 1021 669 1121
Fragments arranged by size:
1121 1021 669
NsiI A_TGCA'T
Cuts at: 0 1108 2616 2744 2811
Size: 1108 1508 128 67
Fragments arranged by size:
1508 1108 128 67
NspI r_CATG'y
Cuts at: 0 1976 2375 2746 2811
Size: 1976 399 371 65
Fragments arranged by size:
1976 399 371 65
PciI A'CATG_T
Cuts at: 0 2371 2811
Size: 2371 440
PfoI T'CCnGG_A
Cuts at: 0 453 1842 2390 2811
Size: 453 1389 548 421
Fragments arranged by size:
1389 548 453 421
PshAI GACnn'nnGTC
Cuts at: 0 2628 2811
Size: 2628 183
PspOMI G'GGCC_C
Cuts at: 0 2494 2811
Size: 2494 317
PstI C_TGCA'G
Cuts at: 0 2319 2811
Size: 2319 492
PvuII CAG'CTG
Cuts at: 0 1021 1690 2811
Size: 1021 669 1121
Fragments arranged by size:
1121 1021 669
SapI GCTCTTCn'nnn_
Cuts at: 0 2675 2811
Size: 2675 136
SexAI A'CCwGG_T
Cuts at: 0 1419 2811
Size: 1419 1392
SfcI C'TryA_G
Cuts at: 0 2113 2315 2633 2811
Size: 2113 202 318 178
Fragments arranged by size:
2113 318 202 178
SmlI C'TyrA_G
Cuts at: 0 1355 2139 2592 2792 2811
Size: 1355 784 453 200 19
Fragments arranged by size:
1355 784 453 200 19
SphI G_CATG'C
Cuts at: 0 2746 2811
Size: 2746 65
SspI AAT'ATT
Cuts at: 0 1481 1632 1833 2811
Size: 1481 151 201 978
Fragments arranged by size:
1481 978 201 151
StuI AGG'CCT
Cuts at: 0 947 2071 2811
Size: 947 1124 740
Fragments arranged by size:
1124 947 740
StyI C'CwwG_G
Cuts at: 0 164 2189 2288 2747 2811
Size: 164 2025 99 459 64
Fragments arranged by size:
2025 459 164 99 64
SwaI ATTT'AAAT
Cuts at: 0 401 2811
Size: 401 2410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2326 2811
Size: 2326 485
TatI w'GTAC_w
Cuts at: 0 23 2374 2811
Size: 23 2351 437
Fragments arranged by size:
2351 437 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2415 2447 2811
Size: 2415 32 364
Fragments arranged by size:
2415 364 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 2131 2811
Size: 378 1753 680
Fragments arranged by size:
1753 680 378
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApoI AseI
BamHI BanII BbsI BbvCI BciVI BclI BfrBI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI
BstAPI BstBI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NsiI
NspI PciI PfoI PshAI PspOMI PstI PvuII SapI
SexAI SfcI SmlI SphI SspI StuI StyI SwaI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AsiSI AvaI AvrII BaeI
BanI BbeI BcgI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI
BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoO109I FseI FspI FspAI HaeII KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI Tth111I XbaI XhoI XmaI XmnI ZraI