Restriction map for pF1KB8491
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AfaI        AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbsI        BcgI        BclI        BlnI        
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HgaI        Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsaI        RsrII       SacII       ScaI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2185  GTCGAC               GT^[AC][GT]AC
AccII        1589  CGCG                 CG^CG
AcsI         2130  GAATTT               [GA]^AATT[CT]
AflII        1105  CTTAAG               C^TTAAG
ApoI         2130  GAATTT               [GA]^AATT[CT]
Asp700I       298  GAAAGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1487  TGGCCA               TGG^CCA
BanI         2663  GGCACC               G^G[CT][GA]CC
BbrPI         338  CACGTG               CAC^GTG
BciVI        1257  GGATAC               GTATCC(6/5)
BfrI         1105  CTTAAG               C^TTAAG
BglI          862  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1905  AGATCT               A^GATCT
BsaI         1198  GAGACC               GGTCTC(1/5)
BsiEI         672  CGACCG               CG[GA][CT]^CG
BsmBI         387  GAGACG               CGTCTC(1/5)
BsmI         1944  GCATTC               GAATGC(1/-1)
BspHI         192  TCATGA               T^CATGA
BspMI        1545  GCAGGT               ACCTGC(4/8)
BstUI        1589  CGCG                 CG^CG
BstXI        1811  CCATGGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT22I       563  ATGCAT               ATGCA^T
HincII       2185  GTCGAC               GT[CT]^[GA]AC
HindII       2185  GTCGAC               GT[CT]^[GA]AC
HindIII       231  AAGCTT               A^AGCTT
MluNI        1487  TGGCCA               TGG^CCA
MscI         1487  TGGCCA               TGG^CCA
MseI         1106  TTAA                 T^TAA
MvnI         1589  CGCG                 CG^CG
NaeI          134  GCCGGC               GCC^GGC
NgoMIV        134  GCCGGC               G^CCGGC
NheI         1497  GCTAGC               G^CTAGC
NsiI          563  ATGCAT               ATGCA^T
PmaCI         338  CACGTG               CAC^GTG
PmlI          338  CACGTG               CAC^GTG
RcaI          192  TCATGA               T^CATGA
SalI         2185  GTCGAC               G^TCGAC
SbfI          987  CCTGCAGG             CCTGCA^GG
SfiI          861  GGCCATGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      987  CCTGCAGG             CCTGCA^GG
Tru9I        1106  TTAA                 T^TAA
XmnI          298  GAAAGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

ApaI         1085  GGGCCC               GGGCC^C
             1923  GGGCCC              
BamHI         357  GGATCC               G^GATCC
             1331  GGATCC              
BsaAI         338  CACGTG               [CT]AC^GT[GA]
             2252  CACGTA              
BsrFI         134  GCCGGC               [GA]^CCGG[CT]
              740  ACCGGC              
Cfr10I        134  GCCGGC               [GA]^CCGG[CT]
              740  ACCGGC              
MaeII         339  ACGT                 A^CGT
             2253  ACGT                
MslI         1678  CACTGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1812  CATGGCCGTG          
PpuMI        1457  AGGACCT              [GA]G^G[AT]CC[CT]
             2315  GGGTCCC             
SacI          802  GAGCTC               GAGCT^C
             1564  GAGCTC              
SapI          994  GAAGAGC              GCTCTTC(1/4)
             1167  GAAGAGC             
SmaI         1577  CCCGGG               CCC^GGG
             1759  CCCGGG              
Tsp45I        100  GTGAC                ^GT[GC]AC
             1375  GTGAC               
XmaCI        1577  CCCGGG               C^CCGGG
             1759  CCCGGG              
XmaI         1577  CCCGGG               C^CCGGG
             1759  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AocI        
AspHI       AvaI        AvaII       BanII       BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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