Restriction map for pF1KB8485
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvrII       BamHI       BciVI       BfrI        BlnI        
BsaBI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
ClaI        DraI        Eco47III    Eco81I      EcoRI       
EcoRV       EcoT22I     FseI        HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        SacII       SalI        ScaI        
SexAI       SfuI        SmlI        SnaBI       SpeI        
SspBI       SspI        SwaI        Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          727  GTAGAC               GT^[AC][GT]AC
Alw44I       1173  GTGCAC               G^TGCAC
ApaI           73  GGGCCC               GGGCC^C
ApaLI        1173  GTGCAC               G^TGCAC
BbrPI        1051  CACGTG               CAC^GTG
BbvCI         183  GCTGAGG              CCTCAGC(-5/-2)
BglII        1691  AGATCT               A^GATCT
BsaI         1453  GAGACC               GGTCTC(1/5)
BsiEI        1166  CGGCCG               CG[GA][CT]^CG
BsmI         1348  GAATGC               GAATGC(1/-1)
BspMI        2412  GCAGGT               ACCTGC(4/8)
BssSI        2762  CTCGTG               CACGAG(-5/-1)
BstEII       2727  GGTCACC              G^GT[ACGT]ACC
BstPI        2727  GGTCACC              G^GT[ACGT]ACC
CpoI          212  CGGTCCG              CG^G[AT]CCG
DrdI         1998  GACCATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1166  CGGCCG               C^GGCCG
EclXI        1166  CGGCCG               C^GGCCG
Eco52I       1166  CGGCCG               C^GGCCG
EcoO65I      2727  GGTCACC              G^GT[ACGT]ACC
HincII       2757  GTTGAC               GT[CT]^[GA]AC
HindII       2757  GTTGAC               GT[CT]^[GA]AC
MluI          743  ACGCGT               A^CGCGT
PmaCI        1051  CACGTG               CAC^GTG
PmlI         1051  CACGTG               CAC^GTG
PshAI        2760  GACTCGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         598  CAGCTG               CAG^CTG
RsrII         212  CGGTCCG              CG^G[AT]CCG
SacI         2083  GAGCTC               GAGCT^C
SapI         2572  GAAGAGC              GCTCTTC(1/4)
SfiI          291  GGCCACAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        1435  CACCGGCG             C[GA]^CCGG[CT]G
SmaI           70  CCCGGG               CCC^GGG
SphI         1559  GCATGC               GCATG^C
TfiI          897  GAATC                G^A[AT]TC
XmaCI          70  CCCGGG               C^CCGGG
XmaI           70  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII        215  TCCGGA               T^CCGGA
             2087  TCCGGA              
AhdI          883  GACCATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2206  GACCAGGCGTC         
AspEI         883  GACCATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2206  GACCAGGCGTC         
AviII         456  TGCGCA               TGC^GCA
             2751  TGCGCA              
BbeI         1439  GGCGCC               GGCGC^C
             2146  GGCGCC              
BbsI          787  GAAGAC               GAAGAC(2/6)
               15  GTCTTC              
BcgI          642  CGACGTGGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2754  GCAGTTGACTCG        
BclI         1482  TGATCA               T^GATCA
             2249  TGATCA              
BlpI         1023  GCTGAGC              GC^T[ACGT]AGC
             2178  GCTCAGC             
Bpu1102I     1023  GCTGAGC              GC^T[ACGT]AGC
             2178  GCTCAGC             
BpuAI         787  GAAGAC               GAAGAC(2/6)
               15  GTCTTC              
BseAI         215  TCCGGA               T^CCGGA
             2087  TCCGGA              
BspEI         215  TCCGGA               T^CCGGA
             2087  TCCGGA              
BsrBI        1291  GAGCGG               CCGCTC(-3/-3)
             1791  GAGCGG              
BsrFI        1436  ACCGGC               [GA]^CCGG[CT]
             2056  ACCGGC              
CelII        1023  GCTGAGC              GC^T[ACGT]AGC
             2178  GCTCAGC             
Cfr10I       1436  ACCGGC               [GA]^CCGG[CT]
             2056  ACCGGC              
DraIII        305  CACTGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1747  CACATCGTG           
Eam1105I      883  GACCATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2206  GACCAGGCGTC         
EarI         2317  CTCTTC               CTCTTC(1/4)
             2572  GAAGAG              
FbaI         1482  TGATCA               T^GATCA
             2249  TGATCA              
FspI          456  TGCGCA               TGC^GCA
             2751  TGCGCA              
KasI         1439  GGCGCC               G^GCGCC
             2146  GGCGCC              
Ksp632I      2317  CTCTTC               CTCTTC(1/4)
             2572  GAAGAG              
MroI          215  TCCGGA               T^CCGGA
             2087  TCCGGA              
MseI         1363  TTAA                 T^TAA
             2299  TTAA                
NarI         1439  GGCGCC               GG^CGCC
             2146  GGCGCC              
NcoI          170  CCATGG               C^CATGG
             1520  CCATGG              
SbfI         1104  CCTGCAGG             CCTGCA^GG
             1317  CCTGCAGG            
SfoI         1439  GGCGCC               GGC^GCC
             2146  GGCGCC              
Sse8387I     1104  CCTGCAGG             CCTGCA^GG
             1317  CCTGCAGG            
Tru9I        1363  TTAA                 T^TAA
             2299  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
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