Restriction map for pF1KB5929
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BcnI        BfrI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaHI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstEII      BstNI       
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRII      EcoT22I     FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
MaeII       MluI        MluNI       MroI        MscI        
MslI        MvaI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        ScrFI       
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       Sse8387I    SspBI       SwaI        
Tsp45I      Tth111I     Van91I      XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I         37  GGTACC               G^GTACC
AlwNI         104  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I        37  GGTACC               G^GTACC
AspHI         365  GTGCTC               G[AT]GC[AT]^C
AvaI           24  CTCGGG               C^[CT]CG[GA]G
AvaII        1164  GGTCC                G^G[AT]CC
BanII          17  GGGCTC               G[GA]GC[CT]^C
BbsI         1004  GAAGAC               GAAGAC(2/6)
BciVI        1242  GGATAC               GTATCC(6/5)
BclI          705  TGATCA               T^GATCA
BpuAI        1004  GAAGAC               GAAGAC(2/6)
BsaI          304  GGTCTC               GGTCTC(1/5)
BsaWI          34  TCCGGT               [AT]^CCGG[AT]
BsgI          869  CTGCAC               GTGCAG(16/14)
BsiHKAI       365  GTGCTC               G[AT]GC[AT]^C
BsmFI          85  GGGAC                GGGAC(10/14)
BsmI          112  GCATTC               GAATGC(1/-1)
BsoBI          24  CTCGGG               C^[CT]CG[GA]G
BspMI         103  GCAGGT               ACCTGC(4/8)
BstAPI        103  GCAGGTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         605  TTCGAA               TT^CGAA
DraII        1163  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     1163  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         487  GAATTC               G^AATTC
EcoRV        1054  GATATC               GAT^ATC
EcoT14I       935  CCAAGG               C^C[AT][AT]GG
FbaI          705  TGATCA               T^GATCA
HapII          35  CCGG                 C^CGG
HpaII          35  CCGG                 C^CGG
HphI         1255  GGTGA                GGTGA(8/7)
KpnI           37  GGTACC               GGTAC^C
MfeI          817  CAATTG               C^AATTG
MspI           35  CCGG                 C^CGG
MunI          817  CAATTG               C^AATTG
NspI          539  GCATGC               [GA]CATG^[CT]
NspV          605  TTCGAA               TT^CGAA
PleI          152  GACTC                GAGTC(4/5)
PpuMI        1163  AGGTCCT              [GA]G^G[AT]CC[CT]
SapI         1219  GAAGAGC              GCTCTTC(1/4)
ScaI          220  AGTACT               AGT^ACT
SfuI          605  TTCGAA               TT^CGAA
SpeI          217  ACTAGT               A^CTAGT
SphI          539  GCATGC               GCATG^C
SspI          472  AATATT               AAT^ATT
StyI          935  CCAAGG               C^C[AT][AT]GG
TspRI         482  CAGTG                CA[GC]TG[ACGT][ACGT]^
XbaI         1452  TCTAGA               T^CTAGA
XcmI         1497  CCACATCAGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          54  CGCG                 CG^CG
               63  CGCG                
AcsI          487  GAATTC               [GA]^AATT[CT]
             1181  AAATTC              
AlwI          913  GATCC                GGATC(4/5)
             1140  GGATC               
ApoI          487  GAATTC               [GA]^AATT[CT]
             1181  AAATTC              
BanI           28  GGCACC               G^G[CT][GA]CC
               37  GGTACC              
BglI           49  GCCACCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1204  GCCATTCAGGC         
BglII        1342  AGATCT               A^GATCT
             1371  AGATCT              
BsaBI        1366  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1431  GATTAGCATC          
BsmAI         305  GTCTC                GTCTC(1/5)
             1305  GTCTC               
BsrDI         635  CATTGC               GCAATG(2/0)
              695  CATTGC              
BstF5I       1494  CATCC                GGATG(2/0)
             1316  GGATG               
BstUI          54  CGCG                 CG^CG
               63  CGCG                
Cac8I         432  GCAGGC               GC[ACGT]^[ACGT]GC
              539  GCATGC              
DsaI           53  CCGCGG               C^C[GA][CT]GG
               62  CCGCGG              
FauI           61  CCCGC                CCCGC(4/6)
               64  GCGGG               
FokI         1494  CATCC                GGATG(9/13)
             1316  GGATG               
KspI           53  CCGCGG               CCGC^GG
               62  CCGCGG              
MaeIII        835  GTAAC                ^GT[ACGT]AC
             1377  GTTAC               
MamI         1366  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1431  GATTAGCATC          
MvnI           54  CGCG                 CG^CG
               63  CGCG                
PvuII         346  CAGCTG               CAG^CTG
             1237  CAGCTG              
SacII          53  CCGCGG               CCGC^GG
               62  CCGCGG              
StuI          434  AGGCCT               AGG^CCT
             1416  AGGCCT              
TaqI          606  TCGA                 T^CGA
             1472  TCGA                
TthHB8I       606  TCGA                 T^CGA
             1472  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BfaI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BstYI       Cfr13I      DdeI        DpnII       
EarI        Fnu4HI      HaeIII      HinfI       ItaI        
Ksp632I     MaeI        MboI        MboII       MflI        
MnlI        MseI        MspA1I      MwoI        NdeII       
NlaIII      NlaIV       PstI        RsaI        Sau3AI      
Sau96I      SfaNI       SfcI        TfiI        Tru9I       
TseI        Tsp509I     XhoII       
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