Restriction map for pF1KB5922
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       CfoI        CpoI        DraI        
DrdI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NarI        NcoI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1592  GGTACC               G^GTACC
AcsI         1245  GAATTC               [GA]^AATT[CT]
Alw44I       1569  GTGCAC               G^TGCAC
AlwI         1419  GATCC                GGATC(4/5)
AlwNI        1295  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1569  GTGCAC               G^TGCAC
ApoI         1245  GAATTC               [GA]^AATT[CT]
Asp718I      1592  GGTACC               G^GTACC
AvaI          823  CTCGGG               C^[CT]CG[GA]G
BbsI          328  GAAGAC               GAAGAC(2/6)
BcgI           51  CGACAGCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          180  CCGGG                CC^[GC]GG
BglII        1033  AGATCT               A^GATCT
BpmI         1082  CTGGAG               CTGGAG(16/14)
BpuAI         328  GAAGAC               GAAGAC(2/6)
BsaBI         967  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1087  GCATTC               GAATGC(1/-1)
BsoBI         823  CTCGGG               C^[CT]CG[GA]G
BspDI         121  ATCGAT               AT^CGAT
BstAPI       1294  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         117  CCACATCGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          121  ATCGAT               AT^CGAT
DraIII       1115  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          606  CGGCCG               C^GGCCG
EclXI         606  CGGCCG               C^GGCCG
Eco52I        606  CGGCCG               C^GGCCG
EcoRI        1245  GAATTC               G^AATTC
HgaI          424  GACGC                GACGC(5/10)
KpnI         1592  GGTACC               GGTAC^C
MaeII        1268  ACGT                 A^CGT
MamI          967  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          118  CACATCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          604  GCCGGC               GCC^GGC
NciI          180  CCGGG                CC^[GC]GG
NdeI         1373  CATATG               CA^TATG
NgoMIV        604  GCCGGC               G^CCGGC
PflMI         922  CCAAAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         1011  GAGCTC               GAGCT^C
SmlI         1145  CTCAAG               C^T[CT][GA]AG
SphI         1581  GCATGC               GCATG^C
Tsp45I        819  GTCAC                ^GT[GC]AC
Tsp509I      1246  AATT                 ^AATT
Van91I        922  CCAAAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          922  CCAAAGCATGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AhdI          379  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1480  GACTTTCTGTC         
AspEI         379  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1480  GACTTTCTGTC         
AspHI        1011  GAGCTC               G[AT]GC[AT]^C
             1569  GTGCAC              
BanII         128  GGGCTC               G[GA]GC[CT]^C
             1011  GAGCTC              
BsaI          573  GAGACC               GGTCTC(1/5)
              978  GAGACC              
BsiEI         522  CGACCG               CG[GA][CT]^CG
              606  CGGCCG              
BsiHKAI      1011  GAGCTC               G[AT]GC[AT]^C
             1569  GTGCAC              
Bsp1407I      515  TGTACA               T^GTACA
             1391  TGTACA              
BsrFI         524  ACCGGC               [GA]^CCGG[CT]
              604  GCCGGC              
BsrGI         515  TGTACA               T^GTACA
             1391  TGTACA              
BsrI         1459  ACTGG                ACTGG(1/-1)
             1566  CCAGT               
BstYI        1033  AGATCT               [GA]^GATC[CT]
             1418  AGATCC              
Cfr10I        524  ACCGGC               [GA]^CCGG[CT]
              604  GCCGGC              
EaeI          581  TGGCCG               [CT]^GGCC[GA]
              606  CGGCCG              
Eam1105I      379  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1480  GACTTTCTGTC         
KspI          265  CCGCGG               CCGC^GG
             1232  CCGCGG              
MaeIII        819  GTCAC                ^GT[ACGT]AC
             1100  GTAAC               
MflI         1033  AGATCT               [GA]^GATC[CT]
             1418  AGATCC              
NspI         1076  ACATGC               [GA]CATG^[CT]
             1581  GCATGC              
PleI         1463  GACTC                GAGTC(4/5)
              385  GAGTC               
PpuMI          32  AGGTCCT              [GA]G^G[AT]CC[CT]
               44  GGGACCC             
PstI          466  CTGCAG               CTGCA^G
             1586  CTGCAG              
SacII         265  CCGCGG               CCGC^GG
             1232  CCGCGG              
SfaNI         239  GCATC                GCATC(5/9)
              972  GCATC               
SfcI          466  CTGCAG               C^T[GA][CT]AG
             1586  CTGCAG              
SspBI         515  TGTACA               T^GTACA
             1391  TGTACA              
XhoII        1033  AGATCT               [GA]^GATC[CT]
             1418  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BanI        BbvI        BfaI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TaqI        TfiI        Tru9I       TseI        TspRI       
TthHB8I     
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