Restriction map for pF1KB5922
 (Linear) (Six-base) MAPSORT of: KIBB5922.seq  Check: 7074  from: 1  to: 1599



KIBB5922.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

Acc65I G'GTAC_C

Cuts at:      0    1592    1599 
   Size:       1592       7
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1497    1599 
   Size:        296    1201     102

  Fragments arranged by size: 

                1201     296     102
 
AhdI GACnn_n'nnGTC

Cuts at:      0     384    1485    1599 
   Size:        384    1101     114

  Fragments arranged by size: 

                1101     384     114
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     913    1599 
   Size:        913     686
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1300    1599 
   Size:       1300     299
 
ApaLI G'TGCA_C

Cuts at:      0    1569    1599 
   Size:       1569      30
 
ApoI r'AATT_y

Cuts at:      0    1245    1599 
   Size:       1245     354
 
AvaI C'yCGr_G

Cuts at:      0     823    1599 
   Size:        823     776
 
BanI G'GyrC_C

Cuts at:      0     183     408    1592    1599 
   Size:        183     225    1184       7

  Fragments arranged by size: 

                1184     225     183       7
 
BanII G_rGCy'C

Cuts at:      0     132    1015    1599 
   Size:        132     883     584

  Fragments arranged by size: 

                 883     584     132
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     335    1599 
   Size:        335    1264
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      40      74    1599 
   Size:         40      34    1525

  Fragments arranged by size: 

                1525      40      34
 
BglII A'GATC_T

Cuts at:      0    1033    1599 
   Size:       1033     566
 
Bme1580I G_kGCm'C

Cuts at:      0      20    1573    1599 
   Size:         20    1553      26

  Fragments arranged by size: 

                1553      26      20
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1103    1599 
   Size:       1103     496
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1130    1599 
   Size:       1130     469
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     567     972    1599 
   Size:        567     405     627

  Fragments arranged by size: 

                 627     567     405
 
BsaBI GATnn'nnATC

Cuts at:      0     971    1599 
   Size:        971     628
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     493     523     705     735    1599 
   Size:        493      30     182      30     864

  Fragments arranged by size: 

                 864     493     182      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     246     249     412    1599 
   Size:        246       3     163    1187

  Fragments arranged by size: 

                1187     246     163       3
 
BseYI C'CCAG_C

Cuts at:      0      41     548     655     989    1599 
   Size:         41     507     107     334     610

  Fragments arranged by size: 

                 610     507     334     107      41
 
BsiEI CG_ry'CG

Cuts at:      0     525     609    1599 
   Size:        525      84     990

  Fragments arranged by size: 

                 990     525      84
 
BsiHKAI G_wGCw'C

Cuts at:      0    1015    1573    1599 
   Size:       1015     558      26

  Fragments arranged by size: 

                1015     558      26
 
BsmI GAATG_Cn'

Cuts at:      0    1087    1599 
   Size:       1087     512
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     132    1015    1573    1599 
   Size:         20     112     883     558      26

  Fragments arranged by size: 

                 883     558     112      26      20
 
BsrFI r'CCGG_y

Cuts at:      0     524     604    1599 
   Size:        524      80     995

  Fragments arranged by size: 

                 995     524      80
 
BsrGI T'GTAC_A

Cuts at:      0     515    1391    1599 
   Size:        515     876     208

  Fragments arranged by size: 

                 876     515     208
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1300    1599 
   Size:       1300     299
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     124    1599 
   Size:        124    1475
 
BstYI r'GATC_y

Cuts at:      0    1033    1418    1599 
   Size:       1033     385     181

  Fragments arranged by size: 

                1033     385     181
 
BtgI C'CryG_G

Cuts at:      0     265     413     869    1232    1599 
   Size:        265     148     456     363     367

  Fragments arranged by size: 

                 456     367     363     265     148
 
BtsI GCAGTG_nn'

Cuts at:      0     110     622    1599 
   Size:        110     512     977

  Fragments arranged by size: 

                 977     512     110
 
ClaI AT'CG_AT

Cuts at:      0     122    1599 
   Size:        122    1477
 
DraIII CAC_nnn'GTG

Cuts at:      0    1120    1599 
   Size:       1120     479
 
EaeI y'GGCC_r

Cuts at:      0     581     606    1599 
   Size:        581      25     993

  Fragments arranged by size: 

                 993     581      25
 
EagI C'GGCC_G

Cuts at:      0     606    1599 
   Size:        606     993
 
EarI CTCTTCn'nnn_

Cuts at:      0     221     272     290     398     942    1235    1253    1599 
   Size:        221      51      18     108     544     293      18     346

  Fragments arranged by size: 

                 544     346     293     221     108      51      18      18
            
 
EcoICRI GAG'CTC

Cuts at:      0    1013    1599 
   Size:       1013     586
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1103    1497    1599 
   Size:        296     807     394     102

  Fragments arranged by size: 

                 807     394     296     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33      45    1150    1599 
   Size:         33      12    1105     449

  Fragments arranged by size: 

                1105     449      33      12
 
EcoRI G'AATT_C

Cuts at:      0    1245    1599 
   Size:       1245     354
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1321    1599 
   Size:       1321     278
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     784     816    1154    1186    1410    1442 
   Size:        370      32     382      32     338      32     224      32

Cuts at:   1442    1471    1503    1599 
   Size:         29      32      96

  Fragments arranged by size: 

                 382     370     338     224      96      32      32      32
                  32      32      29
 
KpnI G_GTAC'C

Cuts at:      0    1596    1599 
   Size:       1596       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    1040    1599 
   Size:        530     510     559

  Fragments arranged by size: 

                 559     530     510
 
MslI CAynn'nnrTG

Cuts at:      0     122    1599 
   Size:        122    1477
 
MspA1I CmG'CkG

Cuts at:      0     267     811    1234    1599 
   Size:        267     544     423     365

  Fragments arranged by size: 

                 544     423     365     267
 
NaeI GCC'GGC

Cuts at:      0     606    1599 
   Size:        606     993
 
NdeI CA'TA_TG

Cuts at:      0    1374    1599 
   Size:       1374     225
 
NgoMIV G'CCGG_C

Cuts at:      0     604    1599 
   Size:        604     995
 
NspI r_CATG'y

Cuts at:      0    1080    1585    1599 
   Size:       1080     505      14

  Fragments arranged by size: 

                1080     505      14
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     928    1599 
   Size:        928     671
 
PpuMI rG'GwC_Cy

Cuts at:      0      33      45    1599 
   Size:         33      12    1554

  Fragments arranged by size: 

                1554      33      12
 
PstI C_TGCA'G

Cuts at:      0     470    1590    1599 
   Size:        470    1120       9

  Fragments arranged by size: 

                1120     470       9
 
SacI G_AGCT'C

Cuts at:      0    1015    1599 
   Size:       1015     584
 
SacII CC_GC'GG

Cuts at:      0     268    1235    1599 
   Size:        268     967     364

  Fragments arranged by size: 

                 967     364     268
 
SanDI GG'GwC_CC

Cuts at:      0      45    1599 
   Size:         45    1554
 
SfcI C'TryA_G

Cuts at:      0     466    1586    1599 
   Size:        466    1120      13

  Fragments arranged by size: 

                1120     466      13
 
SmlI C'TyrA_G

Cuts at:      0    1145    1599 
   Size:       1145     454
 
SphI G_CATG'C

Cuts at:      0    1585    1599 
   Size:       1585      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     591    1599 
   Size:        591    1008
 
TatI w'GTAC_w

Cuts at:      0     515    1391    1599 
   Size:        515     876     208

  Fragments arranged by size: 

                 876     515     208
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     705     737    1599 
   Size:        705      32     862

  Fragments arranged by size: 

                 862     705      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     929    1599 
   Size:        929     670
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI     AlfI    AlwNI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI     BcgI    BglII Bme1580I     BpmI    BpuEI
     BsaI    BsaBI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BsrFI    BsrGI   BstAPI    BstXI    BstYI     BtgI     BtsI
     ClaI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    Hin4I     KpnI     MmeI     MslI   MspA1I     NaeI
     NdeI   NgoMIV     NspI    PflMI    PpuMI     PstI     SacI    SacII
    SanDI     SfcI     SmlI     SphI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI
     BsgI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI    CspCI
     DraI     DrdI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NarI     NcoI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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