Restriction map for pF1KB5913
 (Linear) (Six-base) MAPSORT of: KIBB5913.seq  Check: 3349  from: 1  to: 1512



KIBB5913.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     749    1512 
   Size:        749     763
 
AatII G_ACGT'C

Cuts at:      0     539    1512 
   Size:        539     973
 
AccI GT'mk_AC

Cuts at:      0     449    1512 
   Size:        449    1063
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1512 
   Size:       1084     428
 
AflII C'TTAA_G

Cuts at:      0    1417    1512 
   Size:       1417      95
 
AflIII A'CryG_T

Cuts at:      0     527    1512 
   Size:        527     985
 
AleI CACnn'nnGTG

Cuts at:      0     764    1512 
   Size:        764     748
 
AlwNI CAG_nnn'CTG

Cuts at:      0     125    1006    1062    1443    1512 
   Size:        125     881      56     381      69

  Fragments arranged by size: 

                 881     381     125      69      56
 
ApaI G_GGCC'C

Cuts at:      0     979    1284    1512 
   Size:        979     305     228

  Fragments arranged by size: 

                 979     305     228
 
AvaI C'yCGr_G

Cuts at:      0    1073    1181    1512 
   Size:       1073     108     331

  Fragments arranged by size: 

                1073     331     108
 
BamHI G'GATC_C

Cuts at:      0     387    1512 
   Size:        387    1125
 
BanI G'GyrC_C

Cuts at:      0     670    1512 
   Size:        670     842
 
BanII G_rGCy'C

Cuts at:      0      78     218     866     979    1182    1284    1512 
   Size:         78     140     648     113     203     102     228

  Fragments arranged by size: 

                 648     228     203     140     113     102      78
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1218    1512 
   Size:       1218     294
 
BglI GCCn_nnn'nGGC

Cuts at:      0      73     442    1512 
   Size:         73     369    1070

  Fragments arranged by size: 

                1070     369      73
 
BlpI GC'TnA_GC

Cuts at:      0      77    1187    1512 
   Size:         77    1110     325

  Fragments arranged by size: 

                1110     325      77
 
Bme1580I G_kGCm'C

Cuts at:      0     979    1284    1512 
   Size:        979     305     228

  Fragments arranged by size: 

                 979     305     228
 
BmgBI CAC'GTC

Cuts at:      0     557    1512 
   Size:        557     955
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116    1512 
   Size:        116    1396
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     274    1259    1378    1512 
   Size:        232      42     985     119     134

  Fragments arranged by size: 

                 985     232     134     119      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1235    1512 
   Size:       1235     277
 
BsaHI Gr'CG_yC

Cuts at:      0     536    1512 
   Size:        536     976
 
BsaWI w'CCGG_w

Cuts at:      0     476     875    1322    1512 
   Size:        476     399     447     190

  Fragments arranged by size: 

                 476     447     399     190
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     130     160     314     344     395     425    1512 
   Size:        130      30     154      30      51      30    1087

  Fragments arranged by size: 

                1087     154     130      51      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0     432    1284    1512 
   Size:        432     852     228

  Fragments arranged by size: 

                 852     432     228
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1011    1512 
   Size:        377     634     501

  Fragments arranged by size: 

                 634     501     377
 
BsiEI CG_ry'CG

Cuts at:      0     477    1512 
   Size:        477    1035
 
BsiHKAI G_wGCw'C

Cuts at:      0     218    1182    1512 
   Size:        218     964     330

  Fragments arranged by size: 

                 964     330     218
 
BsmI GAATG_Cn'

Cuts at:      0    1074    1512 
   Size:       1074     438
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     218     866     979    1182    1284    1512 
   Size:         78     140     648     113     203     102     228

  Fragments arranged by size: 

                 648     228     203     140     113     102      78
 
BspEI T'CCGG_A

Cuts at:      0    1322    1512 
   Size:       1322     190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     503     749    1047    1163    1512 
   Size:        503     246     298     116     349

  Fragments arranged by size: 

                 503     349     298     246     116
 
BsrBI CCG'CTC

Cuts at:      0    1191    1512 
   Size:       1191     321
 
BsrDI GCAATG_nn'

Cuts at:      0     218     992    1512 
   Size:        218     774     520

  Fragments arranged by size: 

                 774     520     218
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     634    1512 
   Size:        634     878
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     766    1170    1512 
   Size:        766     404     342

  Fragments arranged by size: 

                 766     404     342
 
BstYI r'GATC_y

Cuts at:      0     387     917    1512 
   Size:        387     530     595

  Fragments arranged by size: 

                 595     530     387
 
Bsu36I CC'TnA_GG

Cuts at:      0    1453    1512 
   Size:       1453      59
 
BtgI C'CryG_G

Cuts at:      0     519     652    1079    1512 
   Size:        519     133     427     433

  Fragments arranged by size: 

                 519     433     427     133
 
BtsI GCAGTG_nn'

Cuts at:      0     205    1214    1512 
   Size:        205    1009     298

  Fragments arranged by size: 

                1009     298     205
 
DraIII CAC_nnn'GTG

Cuts at:      0    1143    1512 
   Size:       1143     369
 
EaeI y'GGCC_r

Cuts at:      0      65     443     649     909    1445    1512 
   Size:         65     378     206     260     536      67

  Fragments arranged by size: 

                 536     378     260     206      67      65
 
EarI CTCTTCn'nnn_

Cuts at:      0     848    1369    1512 
   Size:        848     521     143

  Fragments arranged by size: 

                 848     521     143
 
EcoICRI GAG'CTC

Cuts at:      0     216    1180    1512 
   Size:        216     964     332

  Fragments arranged by size: 

                 964     332     216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     274    1084    1259    1378    1512 
   Size:        232      42     810     175     119     134

  Fragments arranged by size: 

                 810     232     175     134     119      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1018    1417    1512 
   Size:       1018     399      95

  Fragments arranged by size: 

                1018     399      95
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258     266     428     975     976    1167    1280    1281 
   Size:        258       8     162     547       1     191     113       1

Cuts at:   1281    1481    1512 
   Size:        200      31

  Fragments arranged by size: 

                 547     258     200     191     162     113      31       8
                   1       1
 
EcoRV GAT'ATC

Cuts at:      0     548    1512 
   Size:        548     964
 
HaeII r_GCGC'y

Cuts at:      0     363     680    1512 
   Size:        363     317     832

  Fragments arranged by size: 

                 832     363     317
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     395     427    1192    1224    1390    1422 
   Size:        130      32     233      32     765      32     166      32

Cuts at:   1422    1512 
   Size:         90

  Fragments arranged by size: 

                 765     233     166     130      90      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1356    1512 
   Size:       1356     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     128    1512 
   Size:        128    1384
 
MscI TGG'CCA

Cuts at:      0      67    1447    1512 
   Size:         67    1380      65

  Fragments arranged by size: 

                1380      67      65
 
MslI CAynn'nnrTG

Cuts at:      0     764     986    1033    1512 
   Size:        764     222      47     479

  Fragments arranged by size: 

                 764     479     222      47
 
MspA1I CmG'CkG

Cuts at:      0      54     624    1512 
   Size:         54     570     888

  Fragments arranged by size: 

                 888     570      54
 
NcoI C'CATG_G

Cuts at:      0    1079    1512 
   Size:       1079     433
 
NspI r_CATG'y

Cuts at:      0     531    1512 
   Size:        531     981
 
PasI CC'CwG_GG

Cuts at:      0     261    1512 
   Size:        261    1251
 
PciI A'CATG_T

Cuts at:      0     527    1512 
   Size:        527     985
 
PfoI T'CCnGG_A

Cuts at:      0    1040    1236    1394    1512 
   Size:       1040     196     158     118

  Fragments arranged by size: 

                1040     196     158     118
 
PpuMI rG'GwC_Cy

Cuts at:      0     266     428    1512 
   Size:        266     162    1084

  Fragments arranged by size: 

                1084     266     162
 
PspOMI G'GGCC_C

Cuts at:      0     975    1280    1512 
   Size:        975     305     232

  Fragments arranged by size: 

                 975     305     232
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     268     300    1512 
   Size:        268      32    1212

  Fragments arranged by size: 

                1212     268      32
 
PstI C_TGCA'G

Cuts at:      0      41     182     241     971    1002    1058    1512 
   Size:         41     141      59     730      31      56     454

  Fragments arranged by size: 

                 730     454     141      59      56      41      31
 
PvuII CAG'CTG

Cuts at:      0      54     624    1512 
   Size:         54     570     888

  Fragments arranged by size: 

                 888     570      54
 
SacI G_AGCT'C

Cuts at:      0     218    1182    1512 
   Size:        218     964     330

  Fragments arranged by size: 

                 964     330     218
 
SanDI GG'GwC_CC

Cuts at:      0     428    1512 
   Size:        428    1084
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1369    1512 
   Size:       1369     143
 
SbfI CC_TGCA'GG

Cuts at:      0     971    1058    1512 
   Size:        971      87     454

  Fragments arranged by size: 

                 971     454      87
 
ScaI AGT'ACT

Cuts at:      0     286    1048    1512 
   Size:        286     762     464

  Fragments arranged by size: 

                 762     464     286
 
SexAI A'CCwGG_T

Cuts at:      0    1139    1512 
   Size:       1139     373
 
SfcI C'TryA_G

Cuts at:      0      37     178     237     967     998    1054    1512 
   Size:         37     141      59     730      31      56     458

  Fragments arranged by size: 

                 730     458     141      59      56      37      31
 
SmlI C'TyrA_G

Cuts at:      0    1417    1512 
   Size:       1417      95
 
StuI AGG'CCT

Cuts at:      0    1024    1512 
   Size:       1024     488
 
StyI C'CwwG_G

Cuts at:      0     368     782    1079    1163    1512 
   Size:        368     414     297      84     349

  Fragments arranged by size: 

                 414     368     349     297      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     532     953    1259    1512 
   Size:        532     421     306     253

  Fragments arranged by size: 

                 532     421     306     253
 
TatI w'GTAC_w

Cuts at:      0       5     284    1046    1512 
   Size:          5     279     762     466

  Fragments arranged by size: 

                 762     466     279       5
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     184     216     314     346    1512 
   Size:        184      32      98      32    1166

  Fragments arranged by size: 

                1166     184      98      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     817    1512 
   Size:        817     695
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     103    1512 
   Size:        103    1409
 
ZraI GAC'GTC

Cuts at:      0     537    1512 
   Size:        537     975
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII   AflIII     AleI    AlwNI
     ApaI     AvaI    BamHI     BanI    BanII    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PasI     PciI     PfoI    PpuMI   PspOMI     PsrI     PstI
    PvuII     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI    BglII     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BseRI    BsiWI    BsmBI    BspHI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoRI     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PvuI    RsrII    SacII     SalI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
     XhoI     XmaI     XmnI
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