Restriction map for pF1KB5902
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvrII       BalI        BbrPI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BsgI        BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        KpnI        
MamI        MfeI        MluI        MluNI       MscI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII        608  TCCGGA               T^CCGGA
Alw44I        451  GTGCAC               G^TGCAC
AocI          456  CCTCAGG              CC^T[ACGT]AGG
ApaLI         451  GTGCAC               G^TGCAC
AviII         326  TGCGCA               TGC^GCA
BamHI        1084  GGATCC               G^GATCC
BbeI          261  GGCGCC               GGCGC^C
BbsI         1424  GAAGAC               GAAGAC(2/6)
BbvCI          79  GCTGAGG              CCTCAGC(-5/-2)
BlpI          303  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      303  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1424  GAAGAC               GAAGAC(2/6)
BseAI         608  TCCGGA               T^CCGGA
BsiEI         375  CGGCCG               CG[GA][CT]^CG
BsmBI         750  CGTCTC               CGTCTC(1/5)
BspEI         608  TCCGGA               T^CCGGA
BsrDI        1230  GCAATG               GCAATG(2/0)
BssHII        420  GCGCGC               G^CGCGC
BstEII       1076  GGTCACC              G^GT[ACGT]ACC
BstPI        1076  GGTCACC              G^GT[ACGT]ACC
BstXI        1305  CCAACGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        456  CCTCAGG              CC^T[ACGT]AGG
CelII         303  GCTGAGC              GC^T[ACGT]AGC
EagI          375  CGGCCG               C^GGCCG
EclXI         375  CGGCCG               C^GGCCG
Eco52I        375  CGGCCG               C^GGCCG
Eco81I        456  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1076  GGTCACC              G^GT[ACGT]ACC
FspI          326  TGCGCA               TGC^GCA
HaeII         261  GGCGCC               [GA]GCGC^[CT]
HindIII       669  AAGCTT               A^AGCTT
KasI          261  GGCGCC               G^GCGCC
KspI          446  CCGCGG               CCGC^GG
MaeII         749  ACGT                 A^CGT
MroI          608  TCCGGA               T^CCGGA
MslI         1050  CATCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          261  GGCGCC               GG^CGCC
NcoI          698  CCATGG               C^CATGG
SacI         1146  GAGCTC               GAGCT^C
SacII         446  CCGCGG               CCGC^GG
SapI          170  GAAGAGC              GCTCTTC(1/4)
SfoI          261  GGCGCC               GGC^GCC
SgrAI         429  CGCCGGCG             C[GA]^CCGG[CT]G
TfiI         1467  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          261  GGCGCC               G[GA]^CG[CT]C
              427  GACGCC              
AspHI         451  GTGCAC               G[AT]GC[AT]^C
             1146  GAGCTC              
AspI          547  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              867  GACCCGGTC           
BfaI          958  CTAG                 C^TAG
             1292  CTAG                
BsaHI         261  GGCGCC               G[GA]^CG[CT]C
              427  GACGCC              
BsiHKAI       451  GTGCAC               G[AT]GC[AT]^C
             1146  GAGCTC              
BsmAI         932  GAGAC                GTCTC(1/5)
              751  GTCTC               
BspMI         994  ACCTGC               ACCTGC(4/8)
              820  GCAGGT              
BsrFI         377  GCCGGC               [GA]^CCGG[CT]
              430  GCCGGC              
Cfr10I        377  GCCGGC               [GA]^CCGG[CT]
              430  GCCGGC              
DsaI          446  CCGCGG               C^C[GA][CT]GG
              698  CCATGG              
EaeI          228  CGGCCA               [CT]^GGCC[GA]
              375  CGGCCG              
EarI          676  CTCTTC               CTCTTC(1/4)
              170  GAAGAG              
EcoNI         495  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              901  CCTGGGGGAGG         
HgaI          427  GACGC                GACGC(5/10)
              356  GCGTC               
Hin1I         261  GGCGCC               G[GA]^CG[CT]C
              427  GACGCC              
Ksp632I       676  CTCTTC               CTCTTC(1/4)
              170  GAAGAG              
MaeI          958  CTAG                 C^TAG
             1292  CTAG                
NaeI          377  GCCGGC               GCC^GGC
              430  GCCGGC              
NgoMIV        377  GCCGGC               G^CCGGC
              430  GCCGGC              
NlaIII        699  CATG                 CATG^
              759  CATG                
PflFI         547  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              867  GACCCGGTC           
PvuII         361  CAGCTG               CAG^CTG
             1237  CAGCTG              
SfaNI         192  GCATC                GCATC(5/9)
              722  GCATC               
StuI          321  AGGCCT               AGG^CCT
              503  AGGCCT              
TaqI          508  TCGA                 T^CGA
              954  TCGA                
Tth111I       547  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              867  GACCCGGTC           
TthHB8I       508  TCGA                 T^CGA
              954  TCGA                
XcmI          972  CCAGGCCCAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1031  CCACTGTCACATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsaWI       BseRI       BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIV       PflMI       PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        StyI        TseI        Tsp45I      TspRI       
Van91I      XhoII       
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