Restriction map for pF1KB5902
 (Linear) (Six-base) MAPSORT of: KIBB5902.seq  Check: 7609  from: 1  to: 1575



KIBB5902.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1003    1575 
   Size:       1003     572
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555    1575 
   Size:        555    1020
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1043    1575 
   Size:       1043     532
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      30    1575 
   Size:         30    1545
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18      79     595    1123    1575 
   Size:         18      61     516     528     452

  Fragments arranged by size: 

                 528     516     452      61      18
 
ApaI G_GGCC'C

Cuts at:      0     102     281     713    1575 
   Size:        102     179     432     862

  Fragments arranged by size: 

                 862     432     179     102
 
ApaLI G'TGCA_C

Cuts at:      0     451    1575 
   Size:        451    1124
 
AvaI C'yCGr_G

Cuts at:      0    1546    1552    1558    1575 
   Size:       1546       6       6      17

  Fragments arranged by size: 

                1546      17       6       6
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1103    1136    1575 
   Size:       1103      33     439

  Fragments arranged by size: 

                1103     439      33
 
BamHI G'GATC_C

Cuts at:      0    1084    1575 
   Size:       1084     491
 
BanI G'GyrC_C

Cuts at:      0     261    1300    1327    1575 
   Size:        261    1039      27     248

  Fragments arranged by size: 

                1039     261     248      27
 
BanII G_rGCy'C

Cuts at:      0     102     114     281     713     791    1150    1323    1547 
   Size:        102      12     167     432      78     359     173     224

Cuts at:   1547    1553    1559    1575 
   Size:          6       6      16

  Fragments arranged by size: 

                 432     359     224     173     167     102      78      16
                  12       6       6
 
BbeI G_GCGC'C

Cuts at:      0     265    1575 
   Size:        265    1310
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1431    1575 
   Size:       1431     144
 
BbvCI CC'TCA_GC

Cuts at:      0      80    1575 
   Size:         80    1495
 
BlpI GC'TnA_GC

Cuts at:      0     304    1575 
   Size:        304    1271
 
Bme1580I G_kGCm'C

Cuts at:      0     102     281     455     713    1305    1332    1575 
   Size:        102     179     174     258     592      27     243

  Fragments arranged by size: 

                 592     258     243     179     174     102      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558    1575 
   Size:        558    1017
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      52     133     805     836     950    1100    1213 
   Size:         38      14      81     672      31     114     150     113

Cuts at:   1213    1418    1525    1575 
   Size:        205     107      50

  Fragments arranged by size: 

                 672     205     150     114     113     107      81      50
                  38      31      14
 
Bpu10I CC'TnA_GC

Cuts at:      0      80    1575 
   Size:         80    1495
 
BsaHI Gr'CG_yC

Cuts at:      0     262     428    1575 
   Size:        262     166    1147

  Fragments arranged by size: 

                1147     262     166
 
BsaWI w'CCGG_w

Cuts at:      0     608    1515    1539    1575 
   Size:        608     907      24      36

  Fragments arranged by size: 

                 907     608      36      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28    1044    1074    1256    1286    1575 
   Size:         28    1016      30     182      30     289

  Fragments arranged by size: 

                1016     289     182      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     258     887    1185    1513    1575 
   Size:        258     629     298     328      62

  Fragments arranged by size: 

                 629     328     298     258      62
 
BseYI C'CCAG_C

Cuts at:      0     119     411     588     982    1208    1220    1335    1575 
   Size:        119     292     177     394     226      12     115     240

  Fragments arranged by size: 

                 394     292     240     226     177     119     115      12
            
 
BsiEI CG_ry'CG

Cuts at:      0     378    1575 
   Size:        378    1197
 
BsiHKAI G_wGCw'C

Cuts at:      0     455    1150    1575 
   Size:        455     695     425

  Fragments arranged by size: 

                 695     455     425
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     756    1575 
   Size:        756     819
 
Bsp1286I G_dGCh'C

Cuts at:      0     102     114     281     455     713     791    1150    1305 
   Size:        102      12     167     174     258      78     359     155

Cuts at:   1305    1323    1332    1547    1553    1559    1575 
   Size:         18       9     215       6       6      16

  Fragments arranged by size: 

                 359     258     215     174     167     155     102      78
                  18      16      12       9       6       6
 
BspEI T'CCGG_A

Cuts at:      0     608    1575 
   Size:        608     967
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     811    1003    1575 
   Size:        811     192     572

  Fragments arranged by size: 

                 811     572     192
 
BsrDI GCAATG_nn'

Cuts at:      0    1237    1575 
   Size:       1237     338
 
BsrFI r'CCGG_y

Cuts at:      0     377     430    1575 
   Size:        377      53    1145

  Fragments arranged by size: 

                1145     377      53
 
BssHII G'CGCG_C

Cuts at:      0     420    1575 
   Size:        420    1155
 
BstEII G'GTnAC_C

Cuts at:      0    1076    1575 
   Size:       1076     499
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1312    1575 
   Size:       1312     263
 
BstYI r'GATC_y

Cuts at:      0     153     529    1084    1575 
   Size:        153     376     555     491

  Fragments arranged by size: 

                 555     491     376     153
 
Bsu36I CC'TnA_GG

Cuts at:      0     457    1575 
   Size:        457    1118
 
BtgI C'CryG_G

Cuts at:      0     446     698    1575 
   Size:        446     252     877

  Fragments arranged by size: 

                 877     446     252
 
BtsI GCAGTG_nn'

Cuts at:      0     881    1277    1575 
   Size:        881     396     298

  Fragments arranged by size: 

                 881     396     298
 
EaeI y'GGCC_r

Cuts at:      0     228     375    1575 
   Size:        228     147    1200

  Fragments arranged by size: 

                1200     228     147
 
EagI C'GGCC_G

Cuts at:      0     375    1575 
   Size:        375    1200
 
EarI CTCTTCn'nnn_

Cuts at:      0     165     682    1575 
   Size:        165     517     893

  Fragments arranged by size: 

                 893     517     165
 
EcoICRI GAG'CTC

Cuts at:      0    1148    1575 
   Size:       1148     427
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38      52     133     555     805     836     950    1100 
   Size:         38      14      81     422     250      31     114     150

Cuts at:   1100    1213    1418    1525    1575 
   Size:        113     205     107      50

  Fragments arranged by size: 

                 422     250     205     150     114     113     107      81
                  50      38      31      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     499     905    1575 
   Size:        499     406     670

  Fragments arranged by size: 

                 670     499     406
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84     277     284     313     710     866     910     986 
   Size:         84     193       7      29     397     156      44      76

Cuts at:    986    1183    1575 
   Size:        197     392

  Fragments arranged by size: 

                 397     392     197     193     156      84      76      44
                  29       7
 
FspI TGC'GCA

Cuts at:      0     328    1575 
   Size:        328    1247
 
HaeII r_GCGC'y

Cuts at:      0     265    1575 
   Size:        265    1310
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     164     196     297     329     620     652    1147    1179 
   Size:        164      32     101      32     291      32     495      32

Cuts at:   1179    1458    1490    1575 
   Size:        279      32      85

  Fragments arranged by size: 

                 495     291     279     164     101      85      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     669    1575 
   Size:        669     906
 
KasI G'GCGC_C

Cuts at:      0     261    1575 
   Size:        261    1314
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     569    1575 
   Size:        569    1006
 
MslI CAynn'nnrTG

Cuts at:      0    1054    1575 
   Size:       1054     521
 
MspA1I CmG'CkG

Cuts at:      0     217     332     363     448    1239    1575 
   Size:        217     115      31      85     791     336

  Fragments arranged by size: 

                 791     336     217     115      85      31
 
NaeI GCC'GGC

Cuts at:      0     379     432    1575 
   Size:        379      53    1143

  Fragments arranged by size: 

                1143     379      53
 
NarI GG'CG_CC

Cuts at:      0     262    1575 
   Size:        262    1313
 
NcoI C'CATG_G

Cuts at:      0     698    1575 
   Size:        698     877
 
NgoMIV G'CCGG_C

Cuts at:      0     377     430    1575 
   Size:        377      53    1145

  Fragments arranged by size: 

                1145     377      53
 
PasI CC'CwG_GG

Cuts at:      0     713    1089    1575 
   Size:        713     376     486

  Fragments arranged by size: 

                 713     486     376
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     205     237    1123    1575 
   Size:        205      32     886     452

  Fragments arranged by size: 

                 886     452     205      32
 
PfoI T'CCnGG_A

Cuts at:      0     149     315     638    1575 
   Size:        149     166     323     937

  Fragments arranged by size: 

                 937     323     166     149
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1007    1039    1575 
   Size:         30     977      32     536

  Fragments arranged by size: 

                 977     536      32      30
 
PpuMI rG'GwC_Cy

Cuts at:      0      84     313     866     986    1183    1575 
   Size:         84     229     553     120     197     392

  Fragments arranged by size: 

                 553     392     229     197     120      84
 
PspOMI G'GGCC_C

Cuts at:      0      98     277     709    1575 
   Size:         98     179     432     866

  Fragments arranged by size: 

                 866     432     179      98
 
PstI C_TGCA'G

Cuts at:      0      38     133     350    1013    1575 
   Size:         38      95     217     663     562

  Fragments arranged by size: 

                 663     562     217      95      38
 
PvuII CAG'CTG

Cuts at:      0     363    1239    1575 
   Size:        363     876     336

  Fragments arranged by size: 

                 876     363     336
 
SacI G_AGCT'C

Cuts at:      0    1150    1575 
   Size:       1150     425
 
SacII CC_GC'GG

Cuts at:      0     449    1575 
   Size:        449    1126
 
SanDI GG'GwC_CC

Cuts at:      0     313     866    1575 
   Size:        313     553     709

  Fragments arranged by size: 

                 709     553     313
 
SapI GCTCTTCn'nnn_

Cuts at:      0     165    1575 
   Size:        165    1410
 
SfcI C'TryA_G

Cuts at:      0      34     129     346     732    1009    1575 
   Size:         34      95     217     386     277     566

  Fragments arranged by size: 

                 566     386     277     217      95      34
 
SfoI GGC'GCC

Cuts at:      0     263    1575 
   Size:        263    1312
 
SgrAI Cr'CCGG_yG

Cuts at:      0     430    1575 
   Size:        430    1145
 
StuI AGG'CCT

Cuts at:      0     323     505    1575 
   Size:        323     182    1070

  Fragments arranged by size: 

                1070     323     182
 
StyI C'CwwG_G

Cuts at:      0     698    1186    1225    1365    1575 
   Size:        698     488      39     140     210

  Fragments arranged by size: 

                 698     488     210     140      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      36    1575 
   Size:         36    1539
 
TatI w'GTAC_w

Cuts at:      0    1274    1575 
   Size:       1274     301
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1044    1076    1256    1288    1575 
   Size:       1044      32     180      32     287

  Fragments arranged by size: 

                1044     287     180      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     550     870    1575 
   Size:        550     320     705

  Fragments arranged by size: 

                 705     550     320
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     979    1038    1575 
   Size:        979      59     537

  Fragments arranged by size: 

                 979     537      59
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     AloI    AlwNI     ApaI    ApaLI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI   BssHII   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII    Hin4I  HindIII     KasI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     PasI    PflMI     PfoI     PpiI    PpuMI
   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI     SfcI
     SfoI    SgrAI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     ApoI     AscI     AseI    AsiSI    AvrII     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI     BsgI    BsiWI     BsmI    BspHI
    BsrBI    BsrGI    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI    EcoRI    EcoRV     FalI
     FseI    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MscI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SalI     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI
     XmaI     XmnI     ZraI
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