Restriction map for pF1KB5891
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvrII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoT22I     FbaI        FseI        
HaeII       HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          214  GTCGAC               GT^[AC][GT]AC
AcyI         1035  GACGCC               G[GA]^CG[CT]C
AflIII        592  ACACGT               A^C[GA][CT]GT
AhdI         1480  GACGACTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1201  GGGCCC               GGGCC^C
AspEI        1480  GACGACTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1349  TGCGCA               TGC^GCA
BamHI         246  GGATCC               G^GATCC
BanI           32  GGCACC               G^G[CT][GA]CC
BciVI         669  GTATCC               GTATCC(6/5)
BpmI         1028  CTCCAG               CTGGAG(16/14)
BsaBI         987  GATAGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1035  GACGCC               G[GA]^CG[CT]C
BsaI         1025  GGTCTC               GGTCTC(1/5)
BsgI         1226  GTGCAG               GTGCAG(16/14)
BsiEI         216  CGACCG               CG[GA][CT]^CG
BsmI           76  GAATGC               GAATGC(1/-1)
BspHI         512  TCATGA               T^CATGA
CfoI         1350  GCGC                 GCG^C
Eam1105I     1480  GACGACTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         910  CCTCCTCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         817  GAATTC               G^AATTC
EcoRV         508  GATATC               GAT^ATC
FspI         1349  TGCGCA               TGC^GCA
HgaI         1035  GACGC                GACGC(5/10)
HhaI         1350  GCGC                 GCG^C
Hin1I        1035  GACGCC               G[GA]^CG[CT]C
HinP1I       1350  GCGC                 G^CGC
HincII        214  GTCGAC               GT[CT]^[GA]AC
HindII        214  GTCGAC               GT[CT]^[GA]AC
HindIII       325  AAGCTT               A^AGCTT
MamI          987  GATAGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          230  TTAA                 T^TAA
MslI          678  CACTTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII        513  CATG                 CATG^
PstI          493  CTGCAG               CTGCA^G
RcaI          512  TCATGA               T^CATGA
SacI          157  GAGCTC               GAGCT^C
SalI          214  GTCGAC               G^TCGAC
SmaI          940  CCCGGG               CCC^GGG
TfiI          278  GAATC                G^A[AT]TC
Tru9I         230  TTAA                 T^TAA
XmaCI         940  CCCGGG               C^CCGGG
XmaI          940  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          687  GTAC                 GT^AC
             1440  GTAC                
AspHI         157  GAGCTC               G[AT]GC[AT]^C
             1166  GAGCAC              
AvaI          188  CTCGGG               C^[CT]CG[GA]G
              940  CCCGGG              
AvaII         862  GGACC                G^G[AT]CC
             1487  GGTCC               
BalI         1233  TGGCCA               TGG^CCA
             1406  TGGCCA              
BanII         157  GAGCTC               G[GA]GC[CT]^C
             1201  GGGCCC              
BfaI           56  CTAG                 C^TAG
              916  CTAG                
BsaAI         578  CACGTA               [CT]AC^GT[GA]
              593  CACGTA              
BsiHKAI       157  GAGCTC               G[AT]GC[AT]^C
             1166  GAGCAC              
BsmAI        1026  GTCTC                GTCTC(1/5)
             1419  GTCTC               
BsoBI         188  CTCGGG               C^[CT]CG[GA]G
              940  CCCGGG              
BspMI         957  ACCTGC               ACCTGC(4/8)
             1316  ACCTGC              
BsrDI        1428  CATTGC               GCAATG(2/0)
             1397  GCAATG              
EaeI         1233  TGGCCA               [CT]^GGCC[GA]
             1406  TGGCCA              
EarI          522  GAAGAG               CTCTTC(1/4)
              601  GAAGAG              
FauI         1368  GCGGG                CCCGC(4/6)
             1383  GCGGG               
HapII         941  CCGG                 C^CGG
             1198  CCGG                
HpaII         941  CCGG                 C^CGG
             1198  CCGG                
Ksp632I       522  GAAGAG               CTCTTC(1/4)
              601  GAAGAG              
MaeI           56  CTAG                 C^TAG
              916  CTAG                
MluNI        1233  TGGCCA               TGG^CCA
             1406  TGGCCA              
MscI         1233  TGGCCA               TGG^CCA
             1406  TGGCCA              
MspI          941  CCGG                 C^CGG
             1198  CCGG                
RsaI          687  GTAC                 GT^AC
             1440  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MvaI        MwoI        NciI        NdeII       
NlaIV       PleI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       
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