Restriction map for pF1KB5883
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BfrI        
BglII       BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MaeIII      MamI        MfeI        MluI        MseI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp45I      Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccIII        774  TCCGGA               T^CCGGA
AhdI          332  GACAGGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         332  GACAGGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          319  CCCGAG               C^[CT]CG[GA]G
AvrII         618  CCTAGG               C^CTAGG
BciVI         448  GGATAC               GTATCC(6/5)
BlnI          618  CCTAGG               C^CTAGG
BsaWI         774  TCCGGA               [AT]^CCGG[AT]
BseAI         774  TCCGGA               T^CCGGA
BsoBI         319  CCCGAG               C^[CT]CG[GA]G
BspEI         774  TCCGGA               T^CCGGA
BsrDI         979  GCAATG               GCAATG(2/0)
BstAPI        163  GCAAGGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1283  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          753  GCGC                 GCG^C
DsaI           11  CCACGG               C^C[GA][CT]GG
Eam1105I      332  GACAGGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI          753  GCGC                 GCG^C
HinP1I        753  GCGC                 G^CGC
HincII       1093  GTCAAC               GT[CT]^[GA]AC
HindII       1093  GTCAAC               GT[CT]^[GA]AC
MaeII         194  ACGT                 A^CGT
MroI          774  TCCGGA               T^CCGGA
NspI          168  GCATGC               [GA]CATG^[CT]
PleI         1359  GAGTC                GAGTC(4/5)
PstI           42  CTGCAG               CTGCA^G
SapI          233  GAAGAGC              GCTCTTC(1/4)
SmlI         1114  CTTGAG               C^T[CT][GA]AG
SphI          168  GCATGC               GCATG^C
TfiI            4  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          352  GAATTC               [GA]^AATT[CT]
             1392  GAATTC              
AlwNI         823  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1069  CAGAGACTG           
ApoI          352  GAATTC               [GA]^AATT[CT]
             1392  GAATTC              
AspHI         694  GAGCAC               G[AT]GC[AT]^C
             1256  GAGCAC              
BalI           50  TGGCCA               TGG^CCA
              905  TGGCCA              
BcnI          146  CCGGG                CC^[GC]GG
              297  CCGGG               
BglI          219  GCCTGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              842  GCCTCTGTGGC         
BsaI          459  GAGACC               GGTCTC(1/5)
             1293  GGTCTC              
BsgI          673  CTGCAC               GTGCAG(16/14)
             1088  GTGCAG              
BsiHKAI       694  GAGCAC               G[AT]GC[AT]^C
             1256  GAGCAC              
BstYI         546  AGATCC               [GA]^GATC[CT]
             1101  AGATCC              
EaeI           50  TGGCCA               [CT]^GGCC[GA]
              905  TGGCCA              
EcoRI         352  GAATTC               G^AATTC
             1392  GAATTC              
FauI          855  CCCGC                CCCGC(4/6)
             1329  CCCGC               
HinfI           4  GAATC                G^A[ACGT]TC
             1359  GAGTC               
MflI          546  AGATCC               [GA]^GATC[CT]
             1101  AGATCC              
MluNI          50  TGGCCA               TGG^CCA
              905  TGGCCA              
MscI           50  TGGCCA               TGG^CCA
              905  TGGCCA              
MslI          785  CATTCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              805  CACCATCATG          
NciI          146  CCGGG                CC^[GC]GG
              297  CCGGG               
TaqI          356  TCGA                 T^CGA
             1297  TCGA                
Tsp509I       353  AATT                 ^AATT
             1393  AATT                
TthHB8I       356  TCGA                 T^CGA
             1297  TCGA                
XcmI          314  CCACTCCCGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              498  CCAACAGCAGTATGG     
XhoII         546  AGATCC               [GA]^GATC[CT]
             1101  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AvaII       
BanII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflMI       PpuMI       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        TspRI       Van91I      
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