Restriction map for pF1KB5766
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BspEI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
Cfr10I      CpoI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NgoMIV      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   33 enzymes) #
###################################

Asp700I      1102  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        2002  GAGCTC               G[AT]GC[AT]^C
AvaI         1061  CTCGGG               C^[CT]CG[GA]G
BbsI         1707  GAAGAC               GAAGAC(2/6)
BbvCI        1978  CCTCAGC              CCTCAGC(-5/-2)
BcnI          213  CCGGG                CC^[GC]GG
BpuAI        1707  GAAGAC               GAAGAC(2/6)
BsaI         1852  GAGACC               GGTCTC(1/5)
BsiHKAI      2002  GAGCTC               G[AT]GC[AT]^C
BsmBI        1543  CGTCTC               CGTCTC(1/5)
BsmI          693  GAATGC               GAATGC(1/-1)
BsoBI        1061  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1841  TGTACA               T^GTACA
BspDI         306  ATCGAT               AT^CGAT
BspHI        1041  TCATGA               T^CATGA
BsrGI        1841  TGTACA               T^GTACA
BstXI        2117  CCAGACGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          306  ATCGAT               AT^CGAT
EcoRI        1786  GAATTC               G^AATTC
HaeII        2203  AGCGCC               [GA]GCGC^[CT]
HapII         213  CCGG                 C^CGG
HindIII      1796  AAGCTT               A^AGCTT
HpaII         213  CCGG                 C^CGG
MaeII        1258  ACGT                 A^CGT
MspI          213  CCGG                 C^CGG
NciI          213  CCGGG                CC^[GC]GG
NheI         2079  GCTAGC               G^CTAGC
RcaI         1041  TCATGA               T^CATGA
SacI         2002  GAGCTC               GAGCT^C
ScaI         1937  AGTACT               AGT^ACT
SspBI        1841  TGTACA               T^GTACA
SwaI         2028  ATTTAAAT             ATTT^AAAT
XmnI         1102  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI         1489  GAATTT               [GA]^AATT[CT]
             1786  GAATTC              
ApoI         1489  GAATTT               [GA]^AATT[CT]
             1786  GAATTC              
BanII        1862  GAGCCC               G[GA]GC[CT]^C
             2002  GAGCTC              
BmyI         1862  GAGCCC               G[AGT]GC[ACT]^C
             2002  GAGCTC              
BsgI          515  CTGCAC               GTGCAG(16/14)
             2230  GTGCAG              
Bsp1286I     1862  GAGCCC               G[AGT]GC[ACT]^C
             2002  GAGCTC              
BspMI         513  ACCTGC               ACCTGC(4/8)
              421  GCAGGT              
BstF5I        533  CATCC                GGATG(2/0)
             1065  GGATG               
BstNI         705  CCAGG                CC^[AT]GG
             1111  CCTGG               
CfoI          789  GCGC                 GCG^C
             2204  GCGC                
EcoRII        705  CCAGG                ^CC[AT]GG
             1111  CCTGG               
FokI          533  CATCC                GGATG(9/13)
             1065  GGATG               
HhaI          789  GCGC                 GCG^C
             2204  GCGC                
HinP1I        789  GCGC                 G^CGC
             2204  GCGC                
HincII        120  GTTGAC               GT[CT]^[GA]AC
             1561  GTTGAC              
HindII        120  GTTGAC               GT[CT]^[GA]AC
             1561  GTTGAC              
MaeIII         28  GTGAC                ^GT[ACGT]AC
              667  GTCAC               
MslI         1333  CACAGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2223  CACTCAGGTG          
MvaI          705  CCAGG                CC^[AT]GG
             1111  CCTGG               
NcoI          743  CCATGG               C^CATGG
             1388  CCATGG              
NdeI         2042  CATATG               CA^TATG
             2195  CATATG              
Tsp45I         28  GTGAC                ^GT[GC]AC
              667  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AocI        
AvaII       BbvI        BclI        BfaI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsrDI       BsrI        BssKI       Bsu36I      Cac8I       
Cfr13I      DdeI        DpnII       DraI        DsaI        
EarI        Eco81I      EcoT14I     FauI        FbaI        
Fnu4HI      HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MboI        MboII       MnlI        
MseI        MspA1I      MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     
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