Restriction map for
pF1KB5874
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiHKAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MaeII MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 542 GACGCC G[GA]^CG[CT]C
AvaI 38 CCCGGG C^[CT]CG[GA]G
BanI 1303 GGCACC G^G[CT][GA]CC
BglI 617 GCCGAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 542 GACGCC G[GA]^CG[CT]C
BsgI 342 GTGCAG GTGCAG(16/14)
BsiEI 18 CGGCCG CG[GA][CT]^CG
BsmI 1296 GAATGC GAATGC(1/-1)
BsoBI 38 CCCGGG C^[CT]CG[GA]G
Bsp1407I 11 TGTACA T^GTACA
BsrFI 545 GCCGGT [GA]^CCGG[CT]
BsrGI 11 TGTACA T^GTACA
BsrI 1206 CCAGT ACTGG(1/-1)
BstAPI 142 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 777 CCATAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 545 GCCGGT [GA]^CCGG[CT]
DraII 1465 AGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 18 CGGCCG C^GGCCG
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
EcoO109I 1465 AGGTCCC [GA]G^G[ACGT]CC[CT]
Hin1I 542 GACGCC G[GA]^CG[CT]C
MseI 65 TTAA T^TAA
PpuMI 1465 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 542 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 345 CAGCTG CAG^CTG
SapI 972 GAAGAGC GCTCTTC(1/4)
SmaI 38 CCCGGG CCC^GGG
SmlI 1473 CTCAAG C^T[CT][GA]AG
SphI 204 GCATGC GCATG^C
SspBI 11 TGTACA T^GTACA
Tru9I 65 TTAA T^TAA
Tsp45I 388 GTGAC ^GT[GC]AC
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 327 GAATTT [GA]^AATT[CT]
436 GAATTT
AfaI 12 GTAC GT^AC
309 GTAC
Aor51HI 56 AGCGCT AGC^GCT
781 AGCGCT
ApoI 327 GAATTT [GA]^AATT[CT]
436 GAATTT
AspI 841 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1317 GACCCGGTC
BanII 672 GGGCTC G[GA]GC[CT]^C
744 GAGCCC
BbsI 520 GAAGAC GAAGAC(2/6)
1189 GAAGAC
BmyI 672 GGGCTC G[AGT]GC[ACT]^C
744 GAGCCC
BpuAI 520 GAAGAC GAAGAC(2/6)
1189 GAAGAC
BsaBI 1214 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1454 GATCTAGATC
BsaI 1315 GAGACC GGTCTC(1/5)
578 GGTCTC
BsmFI 411 GGGAC GGGAC(10/14)
1467 GTCCC
Bsp1286I 672 GGGCTC G[AGT]GC[ACT]^C
744 GAGCCC
BstF5I 1220 CATCC GGATG(2/0)
480 GGATG
DdeI 25 CTGAG C^T[ACGT]AG
647 CTAAG
EaeI 18 CGGCCG [CT]^GGCC[GA]
273 TGGCCG
Eco47III 56 AGCGCT AGC^GCT
781 AGCGCT
FokI 1220 CATCC GGATG(9/13)
480 GGATG
HaeII 56 AGCGCT [GA]GCGC^[CT]
781 AGCGCT
HphI 184 GGTGA GGTGA(8/7)
429 GGTGA
KspI 4 CCGCGG CCGC^GG
210 CCGCGG
MaeIII 233 GTTAC ^GT[ACGT]AC
388 GTGAC
MamI 1214 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1454 GATCTAGATC
NlaIII 205 CATG CATG^
1249 CATG
NspI 204 GCATGC [GA]CATG^[CT]
1248 ACATGC
PflFI 841 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1317 GACCCGGTC
RsaI 12 GTAC GT^AC
309 GTAC
SacII 4 CCGCGG CCGC^GG
210 CCGCGG
TfiI 1261 GAATC G^A[AT]TC
1369 GAATC
TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^
1194 CACTG
Tth111I 841 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1317 GACCCGGTC
XbaI 1420 TCTAGA T^CTAGA
1456 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BcnI BfaI BglII BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsrBI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII DsaI
EarI EcoRII FauI Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TthHB8I
XhoII