Restriction map for pF1KB5874
 (Linear) (Six-base) MAPSORT of: KIBB5874.seq  Check: 9669  from: 1  to: 1482



KIBB5874.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     509     839    1482 
   Size:        118     391     330     643

  Fragments arranged by size: 

                 643     391     330     118
 
AfeI AGC'GCT

Cuts at:      0      58     783    1482 
   Size:         58     725     699

  Fragments arranged by size: 

                 725     699      58
 
ApoI r'AATT_y

Cuts at:      0     327     436    1482 
   Size:        327     109    1046

  Fragments arranged by size: 

                1046     327     109
 
AvaI C'yCGr_G

Cuts at:      0      38    1482 
   Size:         38    1444
 
BanI G'GyrC_C

Cuts at:      0    1303    1482 
   Size:       1303     179
 
BanII G_rGCy'C

Cuts at:      0     676     748    1482 
   Size:        676      72     734

  Fragments arranged by size: 

                 734     676      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     527    1196    1482 
   Size:        527     669     286

  Fragments arranged by size: 

                 669     527     286
 
BglI GCCn_nnn'nGGC

Cuts at:      0     623    1482 
   Size:        623     859
 
BglII A'GATC_T

Cuts at:      0     655    1213    1459    1482 
   Size:        655     558     246      23

  Fragments arranged by size: 

                 655     558     246      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1200    1482 
   Size:       1200     282
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     674    1482 
   Size:        674     808
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     540    1362    1482 
   Size:        106     434     822     120

  Fragments arranged by size: 

                 822     434     120     106
 
Bpu10I CC'TnA_GC

Cuts at:      0      25    1482 
   Size:         25    1457
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1458    1482 
   Size:       1458      24
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     584    1309    1482 
   Size:        584     725     173

  Fragments arranged by size: 

                 725     584     173
 
BsaBI GATnn'nnATC

Cuts at:      0    1218    1458    1482 
   Size:       1218     240      24

  Fragments arranged by size: 

                1218     240      24
 
BsaHI Gr'CG_yC

Cuts at:      0     543    1482 
   Size:        543     939
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     346     696     726     894     924    1306    1336 
   Size:        316      30     350      30     168      30     382      30

Cuts at:   1336    1482 
   Size:        146

  Fragments arranged by size: 

                 382     350     316     168     146      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     933     943     946    1099    1140    1298    1482 
   Size:        933      10       3     153      41     158     184

  Fragments arranged by size: 

                 933     184     158     153      41      10       3
 
BseYI C'CCAG_C

Cuts at:      0     748    1482 
   Size:        748     734
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363    1482 
   Size:        363    1119
 
BsiEI CG_ry'CG

Cuts at:      0      21    1482 
   Size:         21    1461
 
BsmI GAATG_Cn'

Cuts at:      0    1302    1482 
   Size:       1302     180
 
Bsp1286I G_dGCh'C

Cuts at:      0     676     748    1482 
   Size:        676      72     734

  Fragments arranged by size: 

                 734     676      72
 
BsrBI CCG'CTC

Cuts at:      0     680    1202    1226    1242    1482 
   Size:        680     522      24      16     240

  Fragments arranged by size: 

                 680     522     240      24      16
 
BsrFI r'CCGG_y

Cuts at:      0     545    1482 
   Size:        545     937
 
BsrGI T'GTAC_A

Cuts at:      0      11    1482 
   Size:         11    1471
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     148    1482 
   Size:        148    1334
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     784    1482 
   Size:        784     698
 
BstYI r'GATC_y

Cuts at:      0      80      93     655     827    1213    1333    1423    1459 
   Size:         80      13     562     172     386     120      90      36

Cuts at:   1459    1482 
   Size:         23

  Fragments arranged by size: 

                 562     386     172     120      90      80      36      23
                  13
 
BtgI C'CryG_G

Cuts at:      0       4     210     217    1482 
   Size:          4     206       7    1265

  Fragments arranged by size: 

                1265     206       7       4
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      62    1482 
   Size:         62    1420
 
BtsI GCAGTG_nn'

Cuts at:      0     255    1482 
   Size:        255    1227
 
EaeI y'GGCC_r

Cuts at:      0      18     273    1482 
   Size:         18     255    1209

  Fragments arranged by size: 

                1209     255      18
 
EagI C'GGCC_G

Cuts at:      0      18    1482 
   Size:         18    1464
 
EarI CTCTTCn'nnn_

Cuts at:      0     815     967    1066    1482 
   Size:        815     152      99     416

  Fragments arranged by size: 

                 815     416     152      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     970    1482 
   Size:        970     512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     118     509     540     839    1362    1482 
   Size:        106      12     391      31     299     523     120

  Fragments arranged by size: 

                 523     391     299     120     106      31      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1466    1482 
   Size:       1466      16
 
HaeII r_GCGC'y

Cuts at:      0      60     785    1482 
   Size:         60     725     697

  Fragments arranged by size: 

                 725     697      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     696     728     852     882     884     894 
   Size:        316      32     348      32     124      30       2      10

Cuts at:    894     914     926    1080    1112    1263    1295    1306    1338 
   Size:         20      12     154      32     151      32      11      32

Cuts at:   1338    1482 
   Size:        144

  Fragments arranged by size: 

                 348     316     154     151     144     124      32      32
                  32      32      32      30      20      12      11      10
                   2
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     694     880     970    1482 
   Size:        458     236     186      90     512

  Fragments arranged by size: 

                 512     458     236     186      90
 
MspA1I CmG'CkG

Cuts at:      0       6     212     347    1482 
   Size:          6     206     135    1135

  Fragments arranged by size: 

                1135     206     135       6
 
NspI r_CATG'y

Cuts at:      0     208    1252    1482 
   Size:        208    1044     230

  Fragments arranged by size: 

                1044     230     208
 
PfoI T'CCnGG_A

Cuts at:      0      83     682    1482 
   Size:         83     599     800

  Fragments arranged by size: 

                 800     599      83
 
PpuMI rG'GwC_Cy

Cuts at:      0    1466    1482 
   Size:       1466      16
 
PshAI GACnn'nnGTC

Cuts at:      0     546    1482 
   Size:        546     936
 
PvuII CAG'CTG

Cuts at:      0     347    1482 
   Size:        347    1135
 
SacII CC_GC'GG

Cuts at:      0       7     213    1482 
   Size:          7     206    1269

  Fragments arranged by size: 

                1269     206       7
 
SapI GCTCTTCn'nnn_

Cuts at:      0     967    1482 
   Size:        967     515
 
SmaI CCC'GGG

Cuts at:      0      40    1482 
   Size:         40    1442
 
SmlI C'TyrA_G

Cuts at:      0    1473    1482 
   Size:       1473       9
 
SphI G_CATG'C

Cuts at:      0     208    1482 
   Size:        208    1274
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     650    1400    1482 
   Size:        650     750      82

  Fragments arranged by size: 

                 750     650      82
 
TatI w'GTAC_w

Cuts at:      0      11     308    1482 
   Size:         11     297    1174

  Fragments arranged by size: 

                1174     297      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1462    1482 
   Size:       1462      20
 
Tth111I GACn'n_nGTC

Cuts at:      0     844    1320    1482 
   Size:        844     476     162

  Fragments arranged by size: 

                 844     476     162
 
XbaI T'CTAG_A

Cuts at:      0    1420    1456    1482 
   Size:       1420      36      26

  Fragments arranged by size: 

                1420      36      26
 
XmaI C'CCGG_G

Cuts at:      0      38    1482 
   Size:         38    1444
 

 Enzymes that do cut:

     AcuI     AfeI     ApoI     AvaI     BanI    BanII     BbsI     BglI
    BglII     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI Bsp1286I
    BsrBI    BsrFI    BsrGI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII
    Hin4I     MmeI   MspA1I     NspI     PfoI    PpuMI    PshAI    PvuII
    SacII     SapI     SmaI     SmlI     SphI    TaqII     TatI     TstI
  Tth111I     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmtI    BsaAI
    BsaWI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     SwaI     XcmI     XhoI     XmnI
     ZraI
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