Restriction map for pF1KB5849
 (Linear) (Six-base) MAPSORT of: KIBB5849.seq  Check: 3158  from: 1  to: 738



KIBB5849.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AfeI AGC'GCT

Cuts at:      0     637     738 
   Size:        637     101
 
AleI CACnn'nnGTG

Cuts at:      0     283     738 
   Size:        283     455
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84     181     738 
   Size:         84      97     557

  Fragments arranged by size: 

                 557      97      84
 
AvaI C'yCGr_G

Cuts at:      0     504     595     738 
   Size:        504      91     143

  Fragments arranged by size: 

                 504     143      91
 
BanI G'GyrC_C

Cuts at:      0     568     738 
   Size:        568     170
 
BanII G_rGCy'C

Cuts at:      0     448     587     596     602     738 
   Size:        448     139       9       6     136

  Fragments arranged by size: 

                 448     139     136       9       6
 
BbvCI CC'TCA_GC

Cuts at:      0     254     738 
   Size:        254     484
 
BclI T'GATC_A

Cuts at:      0     287     738 
   Size:        287     451
 
Bme1580I G_kGCm'C

Cuts at:      0     571     738 
   Size:        571     167
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     315     738 
   Size:        315     423
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     462     738 
   Size:        462     276
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     109     738 
   Size:         58      51     629

  Fragments arranged by size: 

                 629      58      51
 
Bpu10I CC'TnA_GC

Cuts at:      0     254     738 
   Size:        254     484
 
BsaWI w'CCGG_w

Cuts at:      0     302     738 
   Size:        302     436
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     243     454     738 
   Size:        243     211     284

  Fragments arranged by size: 

                 284     243     211
 
BseYI C'CCAG_C

Cuts at:      0     130     406     738 
   Size:        130     276     332

  Fragments arranged by size: 

                 332     276     130
 
BsiEI CG_ry'CG

Cuts at:      0     303     738 
   Size:        303     435
 
BsmI GAATG_Cn'

Cuts at:      0     403     738 
   Size:        403     335
 
Bsp1286I G_dGCh'C

Cuts at:      0     448     571     587     596     602     738 
   Size:        448     123      16       9       6     136

  Fragments arranged by size: 

                 448     136     123      16       9       6
 
BsrGI T'GTAC_A

Cuts at:      0     479     738 
   Size:        479     259
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      84     738 
   Size:         84     654
 
BstEII G'GTnAC_C

Cuts at:      0     453     738 
   Size:        453     285
 
BstYI r'GATC_y

Cuts at:      0     529     710     738 
   Size:        529     181      28

  Fragments arranged by size: 

                 529     181      28
 
Bsu36I CC'TnA_GG

Cuts at:      0     431     738 
   Size:        431     307
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     156     738 
   Size:        156     582
 
BtsI GCAGTG_nn'

Cuts at:      0      85     547     738 
   Size:         85     462     191

  Fragments arranged by size: 

                 462     191      85
 
EaeI y'GGCC_r

Cuts at:      0      44     738 
   Size:         44     694
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     220     738 
   Size:         88     132     518

  Fragments arranged by size: 

                 518     132      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     640     738 
   Size:        640      98
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     109     738 
   Size:         58      51     629

  Fragments arranged by size: 

                 629      58      51
 
FspI TGC'GCA

Cuts at:      0     370     738 
   Size:        370     368
 
HaeII r_GCGC'y

Cuts at:      0     639     738 
   Size:        639      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     521     553     571     603     738 
   Size:        521      32      18      32     135

  Fragments arranged by size: 

                 521     135      32      32      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     738 
   Size:        299     439
 
MscI TGG'CCA

Cuts at:      0      46     738 
   Size:         46     692
 
MslI CAynn'nnrTG

Cuts at:      0     283     738 
   Size:        283     455
 
MspA1I CmG'CkG

Cuts at:      0     216     426     738 
   Size:        216     210     312

  Fragments arranged by size: 

                 312     216     210
 
NdeI CA'TA_TG

Cuts at:      0     282     738 
   Size:        282     456
 
NspI r_CATG'y

Cuts at:      0     633     738 
   Size:        633     105
 
PstI C_TGCA'G

Cuts at:      0     529     554     738 
   Size:        529      25     184

  Fragments arranged by size: 

                 529     184      25
 
SfcI C'TryA_G

Cuts at:      0     525     550     738 
   Size:        525      25     188

  Fragments arranged by size: 

                 525     188      25
 
SmaI CCC'GGG

Cuts at:      0     506     738 
   Size:        506     232
 
StuI AGG'CCT

Cuts at:      0     670     738 
   Size:        670      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166     510     738 
   Size:        166     344     228

  Fragments arranged by size: 

                 344     228     166
 
TatI w'GTAC_w

Cuts at:      0     479     738 
   Size:        479     259
 
XmaI C'CCGG_G

Cuts at:      0     504     738 
   Size:        504     234
 

 Enzymes that do cut:

     AfeI     AleI    AlwNI     AvaI     BanI    BanII    BbvCI     BclI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaWI    BseRI    BseYI
    BsiEI     BsmI Bsp1286I    BsrGI   BstAPI   BstEII    BstYI   Bsu36I
    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI     FspI    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NdeI     NspI     PstI
     SfcI     SmaI     StuI    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI    BstXI  BstZ17I     BtgI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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