Restriction map for pF1KB5733
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Aor51HI     ApoI        
AscI        AseI        AsnI        AspEI       AspI        
AvrII       BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        HincII      HindII      HindIII     
HpaI        MfeI        MluI        MroI        MunI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        TfiI        Tth111I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        365  GGTACC               G^GTACC
AcyI         1294  GGCGCC               G[GA]^CG[CT]C
Alw44I       1077  GTGCAC               G^TGCAC
ApaI          762  GGGCCC               GGGCC^C
ApaLI        1077  GTGCAC               G^TGCAC
Asp700I        81  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       365  GGTACC               G^GTACC
BamHI        1623  GGATCC               G^GATCC
BbeI         1294  GGCGCC               GGCGC^C
BbrPI          96  CACGTG               CAC^GTG
BglII         176  AGATCT               A^GATCT
BsaAI          96  CACGTG               [CT]AC^GT[GA]
BsaBI        1618  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1294  GGCGCC               G[GA]^CG[CT]C
BsgI         1813  CTGCAC               GTGCAG(16/14)
Bsp1407I     2246  TGTACA               T^GTACA
BspHI        1598  TCATGA               T^CATGA
BsrBI        1533  CCGCTC               CCGCTC(-3/-3)
BsrDI        1131  CATTGC               GCAATG(2/0)
BsrGI        2246  TGTACA               T^GTACA
Hin1I        1294  GGCGCC               G[GA]^CG[CT]C
KasI         1294  GGCGCC               G^GCGCC
KpnI          365  GGTACC               GGTAC^C
KspI          244  CCGCGG               CCGC^GG
MamI         1618  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         1583  TTAA                 T^TAA
NarI         1294  GGCGCC               GG^CGCC
NdeI         1268  CATATG               CA^TATG
NheI           57  GCTAGC               G^CTAGC
PaeR7I       1393  CTCGAG               C^TCGAG
PmaCI          96  CACGTG               CAC^GTG
PmlI           96  CACGTG               CAC^GTG
RcaI         1598  TCATGA               T^CATGA
SacII         244  CCGCGG               CCGC^GG
SapI         1821  GAAGAGC              GCTCTTC(1/4)
SfoI         1294  GGCGCC               GGC^GCC
SphI          674  GCATGC               GCATG^C
SspBI        2246  TGTACA               T^GTACA
Tru9I        1583  TTAA                 T^TAA
XhoI         1393  CTCGAG               C^TCGAG
XmnI           81  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII         95  ACACGT               A^C[GA][CT]GT
              323  ACGTGT              
AocI         1723  CCTGAGG              CC^T[ACGT]AGG
             2140  CCTGAGG             
AspHI        1077  GTGCAC               G[AT]GC[AT]^C
             1453  GAGCAC              
AviII         107  TGCGCA               TGC^GCA
              671  TGCGCA              
BcgI         1058  CGACTGACTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2067  GCAGCCACCTCG        
BfaI           58  CTAG                 C^TAG
             1645  CTAG                
BsaI         1900  GAGACC               GGTCTC(1/5)
              444  GGTCTC              
BsiHKAI      1077  GTGCAC               G[AT]GC[AT]^C
             1453  GAGCAC              
BspMI        2135  ACCTGC               ACCTGC(4/8)
              294  GCAGGT              
BstYI         176  AGATCT               [GA]^GATC[CT]
             1623  GGATCC              
Bsu36I       1723  CCTGAGG              CC^T[ACGT]AGG
             2140  CCTGAGG             
DrdI          216  GACGAAGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1764  GACCCTCACGTC        
EarI         1821  GAAGAG               CTCTTC(1/4)
             2326  GAAGAG              
Eco81I       1723  CCTGAGG              CC^T[ACGT]AGG
             2140  CCTGAGG             
EcoNI         804  CCTCGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2136  CCTGCCTGAGG         
FseI         1958  GGCCGGCC             GGCCGG^CC
             2151  GGCCGGCC            
FspI          107  TGCGCA               TGC^GCA
              671  TGCGCA              
Ksp632I      1821  GAAGAG               CTCTTC(1/4)
             2326  GAAGAG              
MaeI           58  CTAG                 C^TAG
             1645  CTAG                
MflI          176  AGATCT               [GA]^GATC[CT]
             1623  GGATCC              
PflMI          92  CCAACACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1223  CCAACGACTGG         
PstI          259  CTGCAG               CTGCA^G
             2206  CTGCAG              
SexAI        1184  ACCTGGT              A^CC[AT]GGT
             1511  ACCTGGT             
SmlI          272  CTCAAG               C^T[CT][GA]AG
             1393  CTCGAG              
Tsp509I       496  AATT                 ^AATT
             1318  AATT                
Van91I         92  CCAACACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1223  CCAACGACTGG         
XcmI         1235  CCACCAATGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1298  CCATGGTGGCCCTGG     
XhoII         176  AGATCT               [GA]^GATC[CT]
             1623  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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