Restriction map for pF1KB5733
 (Linear) (Six-base) MAPSORT of: KIBB5733.seq  Check: 5415  from: 1  to: 2382



KIBB5733.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     285    2144    2382 
   Size:        285    1859     238

  Fragments arranged by size: 

                1859     285     238
 
Acc65I G'GTAC_C

Cuts at:      0     365    2382 
   Size:        365    2017
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2312    2382 
   Size:       2312      70
 
AflIII A'CryG_T

Cuts at:      0      95     323    2382 
   Size:         95     228    2059

  Fragments arranged by size: 

                2059     228      95
 
AlwNI CAG_nnn'CTG

Cuts at:      0     285    1108    1757    1919    2382 
   Size:        285     823     649     162     463

  Fragments arranged by size: 

                 823     649     463     285     162
 
ApaI G_GGCC'C

Cuts at:      0     766    2382 
   Size:        766    1616
 
ApaLI G'TGCA_C

Cuts at:      0    1077    2382 
   Size:       1077    1305
 
AvaI C'yCGr_G

Cuts at:      0     766     876    1393    2382 
   Size:        766     110     517     989

  Fragments arranged by size: 

                 989     766     517     110
 
BamHI G'GATC_C

Cuts at:      0    1623    2382 
   Size:       1623     759
 
BanI G'GyrC_C

Cuts at:      0     365    1096    1294    1528    1714    2382 
   Size:        365     731     198     234     186     668

  Fragments arranged by size: 

                 731     668     365     234     198     186
 
BanII G_rGCy'C

Cuts at:      0     338     766     859    1373    1433    2382 
   Size:        338     428      93     514      60     949

  Fragments arranged by size: 

                 949     514     428     338      93      60
 
BbeI G_GCGC'C

Cuts at:      0    1298    2382 
   Size:       1298    1084
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      78    1411    2175    2187    2382 
   Size:         78    1333     764      12     195

  Fragments arranged by size: 

                1333     764     195      78      12
 
BbvCI CC'TCA_GC

Cuts at:      0       7     356    1101    1978    2158    2382 
   Size:          7     349     745     877     180     224

  Fragments arranged by size: 

                 877     745     349     224     180       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1047    1081    2056    2090    2382 
   Size:       1047      34     975      34     292

  Fragments arranged by size: 

                1047     975     292      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     814    1303    1526    1847    2076    2382 
   Size:        814     489     223     321     229     306

  Fragments arranged by size: 

                 814     489     321     306     229     223
 
BglII A'GATC_T

Cuts at:      0     176    2382 
   Size:        176    2206
 
Bme1580I G_kGCm'C

Cuts at:      0     434     766    1081    1099    1531    2314    2382 
   Size:        434     332     315      18     432     783      68

  Fragments arranged by size: 

                 783     434     432     332     315      68      18
 
BmgBI CAC'GTC

Cuts at:      0     324    1772    1896    2382 
   Size:        324    1448     124     486

  Fragments arranged by size: 

                1448     486     324     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1239    1599    2382 
   Size:       1239     360     783

  Fragments arranged by size: 

                1239     783     360
 
BmtI G_CTAG'C

Cuts at:      0      61    2382 
   Size:         61    2321
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     287     729    1014    1644    2382 
   Size:        287     442     285     630     738

  Fragments arranged by size: 

                 738     630     442     287     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137    1165    1471    1727    1829    2382 
   Size:        137    1028     306     256     102     553

  Fragments arranged by size: 

                1028     553     306     256     137     102
 
Bpu10I CC'TnA_GC

Cuts at:      0       7      71     356    1101    1116    1460    1978    2158 
   Size:          7      64     285     745      15     344     518     180

Cuts at:   2158    2382 
   Size:        224

  Fragments arranged by size: 

                 745     518     344     285     224     180      64      15
                   7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257    2382 
   Size:        257    2125
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     450    1894    2382 
   Size:        450    1444     488

  Fragments arranged by size: 

                1444     488     450
 
BsaAI yAC'GTr

Cuts at:      0      98    2382 
   Size:         98    2284
 
BsaBI GATnn'nnATC

Cuts at:      0    1622    2382 
   Size:       1622     760
 
BsaHI Gr'CG_yC

Cuts at:      0    1295    2382 
   Size:       1295    1087
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2299    2329    2382 
   Size:       2299      30      53

  Fragments arranged by size: 

                2299      53      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     224     826    2320    2382 
   Size:        224     602    1494      62

  Fragments arranged by size: 

                1494     602     224      62
 
BseYI C'CCAG_C

Cuts at:      0     123     545     717     758     852    1337    1987    2382 
   Size:        123     422     172      41      94     485     650     395

  Fragments arranged by size: 

                 650     485     422     395     172     123      94      41
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1798    2382 
   Size:       1798     584
 
BsiHKAI G_wGCw'C

Cuts at:      0    1081    1457    2382 
   Size:       1081     376     925

  Fragments arranged by size: 

                1081     925     376
 
Bsp1286I G_dGCh'C

Cuts at:      0     338     434     766     859    1081    1099    1373    1433 
   Size:        338      96     332      93     222      18     274      60

Cuts at:   1433    1457    1531    2314    2382 
   Size:         24      74     783      68

  Fragments arranged by size: 

                 783     338     332     274     222      96      93      74
                  68      60      24      18
 
BspHI T'CATG_A

Cuts at:      0    1598    2382 
   Size:       1598     784
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     285    2144    2382 
   Size:        285    1859     238

  Fragments arranged by size: 

                1859     285     238
 
BsrBI CCG'CTC

Cuts at:      0    1535    2382 
   Size:       1535     847
 
BsrDI GCAATG_nn'

Cuts at:      0    1130    2382 
   Size:       1130    1252
 
BsrFI r'CCGG_y

Cuts at:      0     254    1123    1959    2152    2382 
   Size:        254     869     836     193     230

  Fragments arranged by size: 

                 869     836     254     230     193
 
BsrGI T'GTAC_A

Cuts at:      0    2246    2382 
   Size:       2246     136
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      67    1757    2109    2382 
   Size:         67    1690     352     273

  Fragments arranged by size: 

                1690     352     273      67
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     141    1245    1270    1512    1545    2382 
   Size:        141    1104      25     242      33     837

  Fragments arranged by size: 

                1104     837     242     141      33      25
 
BstYI r'GATC_y

Cuts at:      0     176    1623    2382 
   Size:        176    1447     759

  Fragments arranged by size: 

                1447     759     176
 
Bsu36I CC'TnA_GG

Cuts at:      0    1724    2141    2382 
   Size:       1724     417     241

  Fragments arranged by size: 

                1724     417     241
 
BtgI C'CryG_G

Cuts at:      0     244     825     979    1298    1491    1523    2252    2259 
   Size:        244     581     154     319     193      32     729       7

Cuts at:   2259    2382 
   Size:        123

  Fragments arranged by size: 

                 729     581     319     244     193     154     123      32
                   7
 
BtsI GCAGTG_nn'

Cuts at:      0     367     707    1032    1758    1836    2382 
   Size:        367     340     325     726      78     546

  Fragments arranged by size: 

                 726     546     367     340     325      78
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     262     297     948     983    2382 
   Size:        262      35     651      35    1399

  Fragments arranged by size: 

                1399     651     262      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     590     592    1188    2382 
   Size:        590       2     596    1194

  Fragments arranged by size: 

                1194     596     590       2
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     222    1770    2382 
   Size:        222    1548     612

  Fragments arranged by size: 

                1548     612     222
 
EaeI y'GGCC_r

Cuts at:      0     131     614    1010    1502    2150    2382 
   Size:        131     483     396     492     648     232

  Fragments arranged by size: 

                 648     492     483     396     232     131
 
EarI CTCTTCn'nnn_

Cuts at:      0    1816    2321    2382 
   Size:       1816     505      61

  Fragments arranged by size: 

                1816     505      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137    1165    1471    1727    1829    2312    2382 
   Size:        137    1028     306     256     102     483      70

  Fragments arranged by size: 

                1028     483     306     256     137     102      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     808    2140    2382 
   Size:        808    1332     242

  Fragments arranged by size: 

                1332     808     242
 
EcoO109I rG'GnC_Cy

Cuts at:      0      11     390     534     762     763    1641    2382 
   Size:         11     379     144     228       1     878     741

  Fragments arranged by size: 

                 878     741     379     228     144      11       1
 
FseI GG_CCGG'CC

Cuts at:      0    1963    2156    2382 
   Size:       1963     193     226

  Fragments arranged by size: 

                1963     226     193
 
FspI TGC'GCA

Cuts at:      0     109     673    2382 
   Size:        109     564    1709

  Fragments arranged by size: 

                1709     564     109
 
FspAI rTGC'GCAy

Cuts at:      0     109     673    2382 
   Size:        109     564    1709

  Fragments arranged by size: 

                1709     564     109
 
HaeII r_GCGC'y

Cuts at:      0     959    1298    2378    2382 
   Size:        959     339    1080       4

  Fragments arranged by size: 

                1080     959     339       4
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2299    2331    2382 
   Size:       2299      32      51

  Fragments arranged by size: 

                2299      51      32
 
KasI G'GCGC_C

Cuts at:      0    1294    2382 
   Size:       1294    1088
 
KpnI G_GTAC'C

Cuts at:      0     369    2382 
   Size:        369    2013
 
MscI TGG'CCA

Cuts at:      0     133     616    1012    1504    2382 
   Size:        133     483     396     492     878

  Fragments arranged by size: 

                 878     492     483     396     133
 
MslI CAynn'nnrTG

Cuts at:      0     562    1217    1268    2382 
   Size:        562     655      51    1114

  Fragments arranged by size: 

                1114     655     562      51
 
MspA1I CmG'CkG

Cuts at:      0      64     127     246     387     758     905    1341    1982 
   Size:         64      63     119     141     371     147     436     641

Cuts at:   1982    2162    2211    2382 
   Size:        180      49     171

  Fragments arranged by size: 

                 641     436     371     180     171     147     141     119
                  64      63      49
 
NaeI GCC'GGC

Cuts at:      0     256    1125    1961    2154    2382 
   Size:        256     869     836     193     228

  Fragments arranged by size: 

                 869     836     256     228     193
 
NarI GG'CG_CC

Cuts at:      0    1295    2382 
   Size:       1295    1087
 
NcoI C'CATG_G

Cuts at:      0    1298    1523    2252    2382 
   Size:       1298     225     729     130

  Fragments arranged by size: 

                1298     729     225     130
 
NdeI CA'TA_TG

Cuts at:      0    1269    2382 
   Size:       1269    1113
 
NgoMIV G'CCGG_C

Cuts at:      0     254    1123    1959    2152    2382 
   Size:        254     869     836     193     230

  Fragments arranged by size: 

                 869     836     254     230     193
 
NheI G'CTAG_C

Cuts at:      0      57    2382 
   Size:         57    2325
 
NspI r_CATG'y

Cuts at:      0     678    1179    1952    2382 
   Size:        678     501     773     430

  Fragments arranged by size: 

                 773     678     501     430
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      98    1229    2382 
   Size:         98    1131    1153

  Fragments arranged by size: 

                1153    1131      98
 
PmlI CAC'GTG

Cuts at:      0      98    2382 
   Size:         98    2284
 
PpuMI rG'GwC_Cy

Cuts at:      0     390     534    1641    2382 
   Size:        390     144    1107     741

  Fragments arranged by size: 

                1107     741     390     144
 
PspOMI G'GGCC_C

Cuts at:      0     762    2382 
   Size:        762    1620
 
PspXI vC'TCGA_Gb

Cuts at:      0    1393    2382 
   Size:       1393     989
 
PstI C_TGCA'G

Cuts at:      0     263    2210    2382 
   Size:        263    1947     172

  Fragments arranged by size: 

                1947     263     172
 
PvuII CAG'CTG

Cuts at:      0      64     758     905    1982    2162    2211    2382 
   Size:         64     694     147    1077     180      49     171

  Fragments arranged by size: 

                1077     694     180     171     147      64      49
 
SacII CC_GC'GG

Cuts at:      0     247    2382 
   Size:        247    2135
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1816    2382 
   Size:       1816     566
 
SexAI A'CCwGG_T

Cuts at:      0    1184    1511    2382 
   Size:       1184     327     871

  Fragments arranged by size: 

                1184     871     327
 
SfcI C'TryA_G

Cuts at:      0     259     747    1020    1878    2206    2382 
   Size:        259     488     273     858     328     176

  Fragments arranged by size: 

                 858     488     328     273     259     176
 
SfoI GGC'GCC

Cuts at:      0    1296    2382 
   Size:       1296    1086
 
SmlI C'TyrA_G

Cuts at:      0     272    1393    2382 
   Size:        272    1121     989

  Fragments arranged by size: 

                1121     989     272
 
SphI G_CATG'C

Cuts at:      0     678    2382 
   Size:        678    1704
 
StuI AGG'CCT

Cuts at:      0     817     994    1783    2382 
   Size:        817     177     789     599

  Fragments arranged by size: 

                 817     789     599     177
 
StyI C'CwwG_G

Cuts at:      0      47    1298    1523    2000    2252    2382 
   Size:         47    1251     225     477     252     130

  Fragments arranged by size: 

                1251     477     252     225     130      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      15    2382 
   Size:         15    2367
 
TatI w'GTAC_w

Cuts at:      0     276    2246    2382 
   Size:        276    1970     136

  Fragments arranged by size: 

                1970     276     136
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1242    1305    2382 
   Size:       1242      63    1077

  Fragments arranged by size: 

                1242    1077      63
 
XhoI C'TCGA_G

Cuts at:      0    1393    2382 
   Size:       1393     989
 
XmnI GAAnn'nnTTC

Cuts at:      0      85    2382 
   Size:         85    2297
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI
   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FseI
     FspI    FspAI    HaeII    Hin4I     KasI     KpnI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NspI
    PflMI     PmlI    PpuMI   PspOMI    PspXI     PstI    PvuII    SacII
     SapI    SexAI     SfcI     SfoI     SmlI     SphI     StuI     StyI
    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BciVI     BclI    BfrBI     BlpI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI     DraI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
   HincII  HindIII     HpaI     MfeI     MluI     MmeI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SbfI
     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XmaI     ZraI
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