Restriction map for pF1KB5832
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BbrPI       BbsI        BbvCI       BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoT22I     
FbaI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tru9I       Tsp509I     XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII         157  CGCG                 CG^CG
AcyI         1164  GGCGCC               G[GA]^CG[CT]C
Alw44I        198  GTGCAC               G^TGCAC
AlwNI         560  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         198  GTGCAC               G^TGCAC
AspI          445  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1014  TGGCCA               TGG^CCA
BamHI         378  GGATCC               G^GATCC
BbeI         1164  GGCGCC               GGCGC^C
BcgI          995  GCAGCCTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          992  CTAG                 C^TAG
BglI           29  GCCCGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         169  TACGTG               [CT]AC^GT[GA]
BsaHI        1164  GGCGCC               G[GA]^CG[CT]C
BsaI          692  GAGACC               GGTCTC(1/5)
BseRI         645  CTCCTC               GAGGAG(10/8)
BsmBI         551  CGTCTC               CGTCTC(1/5)
BsmI          733  GCATTC               GAATGC(1/-1)
BspHI        1382  TCATGA               T^CATGA
BsrBI        1027  CCGCTC               CCGCTC(-3/-3)
BstEII        162  GGTCACC              G^GT[ACGT]ACC
BstPI         162  GGTCACC              G^GT[ACGT]ACC
BstUI         157  CGCG                 CG^CG
EaeI         1014  TGGCCA               [CT]^GGCC[GA]
EcoNI         760  CCTTCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       162  GGTCACC              G^GT[ACGT]ACC
EcoRV         175  GATATC               GAT^ATC
EcoT14I       765  CCAAGG               C^C[AT][AT]GG
HaeII        1164  GGCGCC               [GA]GCGC^[CT]
Hin1I        1164  GGCGCC               G[GA]^CG[CT]C
KasI         1164  GGCGCC               G^GCGCC
MaeI          992  CTAG                 C^TAG
MluNI        1014  TGGCCA               TGG^CCA
MscI         1014  TGGCCA               TGG^CCA
MvnI          157  CGCG                 CG^CG
NarI         1164  GGCGCC               GG^CGCC
PaeR7I        576  CTCGAG               C^TCGAG
PflFI         445  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1222  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          891  GACTC                GAGTC(4/5)
RcaI         1382  TCATGA               T^CATGA
SbfI          871  CCTGCAGG             CCTGCA^GG
ScaI          664  AGTACT               AGT^ACT
SfoI         1164  GGCGCC               GGC^GCC
SmlI          576  CTCGAG               C^T[CT][GA]AG
Sse8387I      871  CCTGCAGG             CCTGCA^GG
StyI          765  CCAAGG               C^C[AT][AT]GG
TfiI          370  GATTC                G^A[AT]TC
Tth111I       445  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1222  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          576  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          364  GTAC                 GT^AC
              665  GTAC                
AspHI         198  GTGCAC               G[AT]GC[AT]^C
              624  GAGCAC              
BsiHKAI       198  GTGCAC               G[AT]GC[AT]^C
              624  GAGCAC              
BstAPI        200  GCACCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              733  GCATTCCCTGC         
BstYI         378  GGATCC               [GA]^GATC[CT]
             1364  GGATCT              
DsaI          182  CCGTGG               C^C[GA][CT]GG
              303  CCGTGG              
HinfI         370  GATTC                G^A[ACGT]TC
              891  GACTC               
MaeII         170  ACGT                 A^CGT
              947  ACGT                
MaeIII        163  GTCAC                ^GT[ACGT]AC
             1039  GTCAC               
MflI          378  GGATCC               [GA]^GATC[CT]
             1364  GGATCT              
MslI          165  CACCTACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              486  CACCCCTGTG          
NlaIII        261  CATG                 CATG^
             1383  CATG                
PpuMI         963  GGGACCC              [GA]G^G[AT]CC[CT]
             1019  AGGTCCC             
PvuII         836  CAGCTG               CAG^CTG
             1358  CAGCTG              
RsaI          364  GTAC                 GT^AC
              665  GTAC                
SapI          809  GCTCTTC              GCTCTTC(1/4)
             1301  GCTCTTC             
SfaNI         346  GATGC                GCATC(5/9)
              787  GATGC               
TaqI          143  TCGA                 T^CGA
              577  TCGA                
Tsp45I        163  GTCAC                ^GT[GC]AC
             1039  GTCAC               
TthHB8I       143  TCGA                 T^CGA
              577  TCGA                
XhoII         378  GGATCC               [GA]^GATC[CT]
             1364  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIV       PstI        Sau3AI      Sau96I      ScrFI       
SfcI        SmaI        TseI        TspRI       XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com