(Circular) (Six-base) MAPSORT of: pF1KB5826.seq Check: 9456 from: 1 to: 4538 pF1KB5826 4538 bp With 182 enzymes: SgfI * October 7, 2009 14:17 .. AccI GT'mk_AC Cuts at: 1576 1576 Size: 4538 Acc65I G'GTAC_C Cuts at: 1554 1554 Size: 4538 AclI AA'CG_TT Cuts at: 4378 4378 Size: 4538 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 98 904 961 1291 1428 2363 2795 3542 3810 Size: 806 57 330 137 935 432 747 268 Cuts at: 3810 98 Size: 826 Fragments arranged by size: 935 826 806 747 432 330 268 137 57 AfeI AGC'GCT Cuts at: 277 277 Size: 4538 AflIII A'CryG_T Cuts at: 958 1890 2994 3971 958 Size: 932 1104 977 1525 Fragments arranged by size: 1525 1104 977 932 AgeI A'CCGG_T Cuts at: 2926 2926 Size: 4538 AlwNI CAG_nnn'CTG Cuts at: 212 257 705 1843 3410 212 Size: 45 448 1138 1567 1340 Fragments arranged by size: 1567 1340 1138 448 45 ApaLI G'TGCA_C Cuts at: 3308 3308 Size: 4538 ApoI r'AATT_y Cuts at: 1542 3891 4147 1542 Size: 2349 256 1933 Fragments arranged by size: 2349 1933 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4538 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4538 AvaI C'yCGr_G Cuts at: 1558 1558 Size: 4538 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 493 526 493 Size: 33 4505 BamHI G'GATC_C Cuts at: 981 1519 1563 981 Size: 538 44 3956 Fragments arranged by size: 3956 538 44 BanI G'GyrC_C Cuts at: 812 1105 1279 1311 1554 1968 2216 2251 812 Size: 293 174 32 243 414 248 35 3099 Fragments arranged by size: 3099 414 293 248 243 174 35 32 BanII G_rGCy'C Cuts at: 791 1552 2582 791 Size: 761 1030 2747 Fragments arranged by size: 2747 1030 761 BbeI G_GCGC'C Cuts at: 1972 2220 1972 Size: 248 4290 BbvCI CC'TCA_GC Cuts at: 952 952 Size: 4538 BciVI GTATCCnnnnn_n' Cuts at: 2436 3197 4538 2436 Size: 761 1341 2436 Fragments arranged by size: 2436 1341 761 BclI T'GATC_A Cuts at: 2058 2058 Size: 4538 BfrBI ATG'CAT Cuts at: 224 224 Size: 4538 BglI GCCn_nnn'nGGC Cuts at: 760 760 Size: 4538 BglII A'GATC_T Cuts at: 2053 4090 2053 Size: 2037 2501 BlpI GC'TnA_GC Cuts at: 822 1650 822 Size: 828 3710 Bme1580I G_kGCm'C Cuts at: 367 453 1110 1211 2163 2256 3312 367 Size: 86 657 101 952 93 1056 1593 Fragments arranged by size: 1593 1056 952 657 101 93 86 BmrI ACTGGGnnnn_n' Cuts at: 447 1146 1750 1831 2166 4310 447 Size: 699 604 81 335 2144 675 Fragments arranged by size: 2144 699 675 604 335 81 BmtI G_CTAG'C Cuts at: 316 316 Size: 4538 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 810 2905 810 Size: 2095 2443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 508 727 890 508 Size: 219 163 4156 Fragments arranged by size: 4156 219 163 Bpu10I CC'TnA_GC Cuts at: 952 952 Size: 4538 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 271 511 1714 2593 3085 3383 271 Size: 240 1203 879 492 298 1426 Fragments arranged by size: 1426 1203 879 492 298 240 BsaI GGTCTCn'nnnn_ Cuts at: 1365 4242 1365 Size: 2877 1661 BsaAI yAC'GTr Cuts at: 1891 1891 Size: 4538 BsaBI GATnn'nnATC Cuts at: 6 1088 2052 4089 6 Size: 1082 964 2037 455 Fragments arranged by size: 2037 1082 964 455 BsaHI Gr'CG_yC Cuts at: 1969 2217 2919 4415 1969 Size: 248 702 1496 2092 Fragments arranged by size: 2092 1496 702 248 BsaWI w'CCGG_w Cuts at: 1727 2248 2926 3200 3347 3758 1727 Size: 521 678 274 147 411 2507 Fragments arranged by size: 2507 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1372 1402 1372 Size: 30 4508 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 157 508 157 Size: 351 4187 BseYI C'CCAG_C Cuts at: 440 764 1049 1109 1963 2721 3298 3794 440 Size: 324 285 60 854 758 577 496 1184 Fragments arranged by size: 1184 854 758 577 496 324 285 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 404 485 676 983 404 Size: 81 191 307 3959 Fragments arranged by size: 3959 307 191 81 BsiEI CG_ry'CG Cuts at: 104 2080 2126 3334 104 Size: 1976 46 1208 1308 Fragments arranged by size: 1976 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 791 1552 2330 2520 3312 791 Size: 761 778 190 792 2017 Fragments arranged by size: 2017 792 778 761 190 BsmI GAATG_Cn' Cuts at: 226 226 Size: 4538 Bsp1286I G_dGCh'C Cuts at: 367 453 791 1110 1211 1552 2163 2256 2330 Size: 86 338 319 101 341 611 93 74 Cuts at: 2330 2520 2582 3312 367 Size: 190 62 730 1593 Fragments arranged by size: 1593 730 611 341 338 319 190 101 93 86 74 62 BspEI T'CCGG_A Cuts at: 3758 3758 Size: 4538 BspHI T'CATG_A Cuts at: 3714 3714 Size: 4538 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1588 1802 2104 2485 1588 Size: 214 302 381 3641 Fragments arranged by size: 3641 381 302 214 BsrBI CCG'CTC Cuts at: 50 2830 2884 4368 4533 50 Size: 2780 54 1484 165 55 Fragments arranged by size: 2780 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2450 2450 Size: 4538 BsrFI r'CCGG_y Cuts at: 269 2536 2717 2926 269 Size: 2267 181 209 1881 Fragments arranged by size: 2267 1881 209 181 BssSI C'ACGA_G Cuts at: 2809 3167 2809 Size: 358 4180 BstAPI GCAn_nnn'nTGC Cuts at: 1215 1915 1215 Size: 700 3838 BstBI TT'CG_AA Cuts at: 2899 2899 Size: 4538 BstYI r'GATC_y Cuts at: 1 981 1519 1563 2030 2053 2388 2634 3646 Size: 980 538 44 467 23 335 246 1012 Cuts at: 3646 3732 3744 4090 1 Size: 86 12 346 449 Fragments arranged by size: 1012 980 538 467 449 346 335 246 86 44 23 12 BtgI C'CryG_G Cuts at: 107 479 1000 1383 107 Size: 372 521 383 3262 Fragments arranged by size: 3262 521 383 372 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2488 2669 2832 2488 Size: 181 163 4194 Fragments arranged by size: 4194 181 163 BtsI GCAGTG_nn' Cuts at: 392 1781 392 Size: 1389 3149 DraI TTT'AAA Cuts at: 1537 1537 Size: 4538 DrdI GACnn_nn'nnGTC Cuts at: 1177 2244 3102 1177 Size: 1067 858 2613 Fragments arranged by size: 2613 1067 858 EaeI y'GGCC_r Cuts at: 201 2123 2297 2688 2715 4060 201 Size: 1922 174 391 27 1345 679 Fragments arranged by size: 1922 1345 679 391 174 27 EagI C'GGCC_G Cuts at: 2123 2123 Size: 4538 EarI CTCTTCn'nnn_ Cuts at: 1067 2561 2771 1067 Size: 1494 210 2834 Fragments arranged by size: 2834 1494 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2746 3056 3202 4348 2746 Size: 310 146 1146 2936 Fragments arranged by size: 2936 1146 310 146 EcoICRI GAG'CTC Cuts at: 789 1550 789 Size: 761 3777 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 98 508 727 890 904 961 1291 1428 2363 Size: 410 219 163 14 57 330 137 935 Cuts at: 2363 2795 3542 3810 98 Size: 432 747 268 826 Fragments arranged by size: 935 826 747 432 410 330 268 219 163 137 57 14 EcoO109I rG'GnC_Cy Cuts at: 432 777 888 1170 1426 1677 432 Size: 345 111 282 256 251 3293 Fragments arranged by size: 3293 345 282 256 251 111 EcoRI G'AATT_C Cuts at: 1542 1542 Size: 4538 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1054 1227 1251 2557 1054 Size: 173 24 1306 3035 Fragments arranged by size: 3035 1306 173 24 FspI TGC'GCA Cuts at: 1198 2319 1198 Size: 1121 3417 HaeII r_GCGC'y Cuts at: 279 1972 2220 3242 4221 279 Size: 1693 248 1022 979 596 Fragments arranged by size: 1693 1022 979 596 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 886 918 1372 1404 2380 2410 2412 2442 886 Size: 32 454 32 976 30 2 30 2982 Fragments arranged by size: 2982 976 454 32 32 30 30 2 HincII GTy'rAC Cuts at: 1577 1577 Size: 4538 KasI G'GCGC_C Cuts at: 1968 2216 1968 Size: 248 4290 KpnI G_GTAC'C Cuts at: 1558 1558 Size: 4538 MluI A'CGCG_T Cuts at: 3971 3971 Size: 4538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 554 3209 3393 554 Size: 2655 184 1699 Fragments arranged by size: 2655 1699 184 MscI TGG'CCA Cuts at: 203 2299 203 Size: 2096 2442 MslI CAynn'nnrTG Cuts at: 2527 2654 2527 Size: 127 4411 MspA1I CmG'CkG Cuts at: 696 729 1649 1963 2323 3336 3581 696 Size: 33 920 314 360 1013 245 1653 Fragments arranged by size: 1653 1013 920 360 314 245 33 NaeI GCC'GGC Cuts at: 271 2719 271 Size: 2448 2090 NarI GG'CG_CC Cuts at: 1969 2217 1969 Size: 248 4290 NcoI C'CATG_G Cuts at: 107 479 1383 107 Size: 372 904 3262 Fragments arranged by size: 3262 904 372 NgoMIV G'CCGG_C Cuts at: 269 2717 269 Size: 2448 2090 NheI G'CTAG_C Cuts at: 312 312 Size: 4538 NsiI A_TGCA'T Cuts at: 226 226 Size: 4538 NspI r_CATG'y Cuts at: 155 962 1591 2091 2998 155 Size: 807 629 500 907 1695 Fragments arranged by size: 1695 907 807 629 500 PciI A'CATG_T Cuts at: 958 2994 958 Size: 2036 2502 PflMI CCAn_nnn'nTGG Cuts at: 480 1001 480 Size: 521 4017 PfoI T'CCnGG_A Cuts at: 857 874 1337 857 Size: 17 463 4058 Fragments arranged by size: 4058 463 17 PmeI GTTT'AAAC Cuts at: 1537 1537 Size: 4538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 3677 Size: 32 4506 PpuMI rG'GwC_Cy Cuts at: 888 1170 1426 888 Size: 282 256 4000 Fragments arranged by size: 4000 282 256 PstI C_TGCA'G Cuts at: 701 842 1186 1585 2270 701 Size: 141 344 399 685 2969 Fragments arranged by size: 2969 685 399 344 141 PvuI CG_AT'CG Cuts at: 104 104 Size: 4538 PvuII CAG'CTG Cuts at: 696 729 1963 2323 696 Size: 33 1234 360 2911 Fragments arranged by size: 2911 1234 360 33 RsrII CG'GwC_CG Cuts at: 2733 2733 Size: 4538 SacI G_AGCT'C Cuts at: 791 1552 791 Size: 761 3777 SalI G'TCGA_C Cuts at: 1575 1575 Size: 4538 SapI GCTCTTCn'nnn_ Cuts at: 2561 2771 2561 Size: 210 4328 SbfI CC_TGCA'GG Cuts at: 1585 1585 Size: 4538 ScaI AGT'ACT Cuts at: 4055 4055 Size: 4538 SfcI C'TryA_G Cuts at: 33 697 781 838 1182 1581 2266 3259 3450 Size: 664 84 57 344 399 685 993 191 Cuts at: 3450 33 Size: 1121 Fragments arranged by size: 1121 993 685 664 399 344 191 84 57 SfiI GGCCn_nnn'nGGCC Cuts at: 760 760 Size: 4538 SfoI GGC'GCC Cuts at: 1970 2218 1970 Size: 248 4290 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4538 SmaI CCC'GGG Cuts at: 1560 1560 Size: 4538 SmlI C'TyrA_G Cuts at: 286 490 1693 2608 3100 3362 286 Size: 204 1203 915 492 262 1462 Fragments arranged by size: 1462 1203 915 492 262 204 SpeI A'CTAG_T Cuts at: 67 67 Size: 4538 SphI G_CATG'C Cuts at: 1591 2091 1591 Size: 500 4038 SspI AAT'ATT Cuts at: 1506 1506 Size: 4538 StuI AGG'CCT Cuts at: 1165 1165 Size: 4538 StyI C'CwwG_G Cuts at: 107 479 1383 1672 2026 107 Size: 372 904 289 354 2619 Fragments arranged by size: 2619 904 372 354 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1063 1093 1165 2121 2254 2713 2922 1063 Size: 30 72 956 133 459 209 2679 Fragments arranged by size: 2679 956 459 209 133 72 30 TatI w'GTAC_w Cuts at: 4053 4053 Size: 4538 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 453 485 966 981 998 1013 4055 4087 453 Size: 32 481 15 17 15 3042 32 904 Fragments arranged by size: 3042 904 481 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 2335 2335 Size: 4538 XbaI T'CTAG_A Cuts at: 1569 1569 Size: 4538 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2033 2033 Size: 4538 XmaI C'CCGG_G Cuts at: 1558 1558 Size: 4538 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BbsI BcgI BmgBI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI NruI PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI