(Circular) (Six-base) MAPSORT of: pF1KB5807.seq Check: 3930 from: 1 to: 4529 pF1KB5807 4529 bp With 182 enzymes: SgfI * October 7, 2009 14:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 124 124 Size: 4529 AccI GT'mk_AC Cuts at: 1567 1567 Size: 4529 Acc65I G'GTAC_C Cuts at: 557 1545 557 Size: 988 3541 AclI AA'CG_TT Cuts at: 1523 4369 1523 Size: 2846 1683 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 309 853 874 2354 2786 3533 3801 309 Size: 544 21 1480 432 747 268 1037 Fragments arranged by size: 1480 1037 747 544 432 268 21 AflIII A'CryG_T Cuts at: 1881 2985 3962 1881 Size: 1104 977 2448 Fragments arranged by size: 2448 1104 977 AgeI A'CCGG_T Cuts at: 2917 2917 Size: 4529 AhdI GACnn_n'nnGTC Cuts at: 1196 1196 Size: 4529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 590 590 Size: 4529 AlwNI CAG_nnn'CTG Cuts at: 1128 1834 3401 1128 Size: 706 1567 2256 Fragments arranged by size: 2256 1567 706 ApaLI G'TGCA_C Cuts at: 1471 3299 1471 Size: 1828 2701 ApoI r'AATT_y Cuts at: 1533 3882 4138 1533 Size: 2349 256 1924 Fragments arranged by size: 2349 1924 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4529 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4529 AvaI C'yCGr_G Cuts at: 1549 1549 Size: 4529 BamHI G'GATC_C Cuts at: 1554 1554 Size: 4529 BanI G'GyrC_C Cuts at: 557 1545 1959 2207 2242 557 Size: 988 414 248 35 2844 Fragments arranged by size: 2844 988 414 248 35 BanII G_rGCy'C Cuts at: 157 1109 1543 2573 157 Size: 952 434 1030 2113 Fragments arranged by size: 2113 1030 952 434 BbeI G_GCGC'C Cuts at: 1963 2211 1963 Size: 248 4281 BbsI GAAGACnn'nnnn_ Cuts at: 1327 1327 Size: 4529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1492 1526 1492 Size: 34 4495 BciVI GTATCCnnnnn_n' Cuts at: 2427 3188 4529 2427 Size: 761 1341 2427 Fragments arranged by size: 2427 1341 761 BclI T'GATC_A Cuts at: 2049 2049 Size: 4529 BglI GCCn_nnn'nGGC Cuts at: 691 691 Size: 4529 BglII A'GATC_T Cuts at: 1097 2044 4081 1097 Size: 947 2037 1545 Fragments arranged by size: 2037 1545 947 BlpI GC'TnA_GC Cuts at: 1153 1641 1153 Size: 488 4041 Bme1580I G_kGCm'C Cuts at: 1475 2154 2247 3303 1475 Size: 679 93 1056 2701 Fragments arranged by size: 2701 1056 679 93 BmgBI CAC'GTC Cuts at: 1317 1317 Size: 4529 BmrI ACTGGGnnnn_n' Cuts at: 1389 1741 1822 2157 4301 1389 Size: 352 81 335 2144 1617 Fragments arranged by size: 2144 1617 352 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2896 2896 Size: 4529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 123 174 535 832 1150 1192 1204 1351 1384 Size: 51 361 297 318 42 12 147 33 Cuts at: 1384 1422 123 Size: 38 3230 Fragments arranged by size: 3230 361 318 297 147 51 42 38 33 12 Bpu10I CC'TnA_GC Cuts at: 946 946 Size: 4529 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1705 2584 3076 3374 1705 Size: 879 492 298 2860 Fragments arranged by size: 2860 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1183 4233 1183 Size: 3050 1479 BsaAI yAC'GTr Cuts at: 1882 1882 Size: 4529 BsaBI GATnn'nnATC Cuts at: 6 2043 4080 6 Size: 2037 2037 455 Fragments arranged by size: 2037 2037 455 BsaHI Gr'CG_yC Cuts at: 1376 1960 2208 2910 4406 1376 Size: 584 248 702 1496 1499 Fragments arranged by size: 1499 1496 702 584 248 BsaWI w'CCGG_w Cuts at: 1718 2239 2917 3191 3338 3749 1718 Size: 521 678 274 147 411 2498 Fragments arranged by size: 2498 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1372 1402 1372 Size: 30 4499 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 126 192 270 532 853 883 886 1054 1201 Size: 66 78 262 321 30 3 168 147 Cuts at: 1201 1264 1377 1380 1392 1425 126 Size: 63 113 3 12 33 3230 Fragments arranged by size: 3230 321 262 168 147 113 78 66 63 33 30 12 3 3 BseYI C'CCAG_C Cuts at: 235 1148 1262 1954 2712 3289 3785 235 Size: 913 114 692 758 577 496 979 Fragments arranged by size: 979 913 758 692 577 496 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 914 1099 914 Size: 185 4344 BsiEI CG_ry'CG Cuts at: 104 480 2071 2117 3325 104 Size: 376 1591 46 1208 1308 Fragments arranged by size: 1591 1308 1208 376 46 BsiHKAI G_wGCw'C Cuts at: 513 1109 1475 1543 2321 2511 3303 513 Size: 596 366 68 778 190 792 1739 Fragments arranged by size: 1739 792 778 596 366 190 68 BsmI GAATG_Cn' Cuts at: 885 953 885 Size: 68 4461 Bsp1286I G_dGCh'C Cuts at: 157 513 1109 1475 1543 2154 2247 2321 2511 Size: 356 596 366 68 611 93 74 190 Cuts at: 2511 2573 3303 157 Size: 62 730 1383 Fragments arranged by size: 1383 730 611 596 366 356 190 93 74 68 62 BspEI T'CCGG_A Cuts at: 3749 3749 Size: 4529 BspHI T'CATG_A Cuts at: 3705 3705 Size: 4529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 124 743 1494 1579 1793 2095 2476 124 Size: 619 751 85 214 302 381 2177 Fragments arranged by size: 2177 751 619 381 302 214 85 BsrBI CCG'CTC Cuts at: 50 1085 2821 2875 4359 4524 50 Size: 1035 1736 54 1484 165 55 Fragments arranged by size: 1736 1484 1035 165 55 54 BsrDI GCAATG_nn' Cuts at: 2441 2441 Size: 4529 BsrFI r'CCGG_y Cuts at: 2527 2708 2917 2527 Size: 181 209 4139 Fragments arranged by size: 4139 209 181 BssSI C'ACGA_G Cuts at: 865 1498 2800 3158 865 Size: 633 1302 358 2236 Fragments arranged by size: 2236 1302 633 358 BstAPI GCAn_nnn'nTGC Cuts at: 1906 1906 Size: 4529 BstBI TT'CG_AA Cuts at: 2890 2890 Size: 4529 BstEII G'GTnAC_C Cuts at: 456 456 Size: 4529 BstXI CCAn_nnnn'nTGG Cuts at: 901 1464 901 Size: 563 3966 BstYI r'GATC_y Cuts at: 1 545 1097 1223 1304 1554 2021 2044 2379 Size: 544 552 126 81 250 467 23 335 Cuts at: 2379 2625 3637 3723 3735 4081 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 552 544 467 449 346 335 250 246 126 86 81 23 12 Bsu36I CC'TnA_GG Cuts at: 622 622 Size: 4529 BtgI C'CryG_G Cuts at: 695 695 Size: 4529 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2479 2660 2823 2479 Size: 181 163 4185 Fragments arranged by size: 4185 181 163 BtsI GCAGTG_nn' Cuts at: 453 1772 453 Size: 1319 3210 DraI TTT'AAA Cuts at: 1528 1528 Size: 4529 DrdI GACnn_nn'nnGTC Cuts at: 2235 3093 2235 Size: 858 3671 EaeI y'GGCC_r Cuts at: 212 692 797 1058 2114 2288 2679 2706 4051 Size: 480 105 261 1056 174 391 27 1345 Cuts at: 4051 212 Size: 690 Fragments arranged by size: 1345 1056 690 480 391 261 174 105 27 EagI C'GGCC_G Cuts at: 2114 2114 Size: 4529 EarI CTCTTCn'nnn_ Cuts at: 2552 2762 2552 Size: 210 4319 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2737 3047 3193 4339 2737 Size: 310 146 1146 2927 Fragments arranged by size: 2927 1146 310 146 EcoICRI GAG'CTC Cuts at: 1107 1541 1107 Size: 434 4095 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 123 174 309 535 832 853 874 1150 1192 Size: 51 135 226 297 21 21 276 42 Cuts at: 1192 1204 1351 1384 1422 2354 2786 3533 3801 Size: 12 147 33 38 932 432 747 268 Cuts at: 3801 123 Size: 851 Fragments arranged by size: 932 851 747 432 297 276 268 226 147 135 51 42 38 33 21 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 499 835 1363 499 Size: 336 528 3665 Fragments arranged by size: 3665 528 336 EcoO109I rG'GnC_Cy Cuts at: 221 618 1506 1668 221 Size: 397 888 162 3082 Fragments arranged by size: 3082 888 397 162 EcoRI G'AATT_C Cuts at: 1533 1533 Size: 4529 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2548 Size: 4529 FspI TGC'GCA Cuts at: 739 772 2310 739 Size: 33 1538 2958 Fragments arranged by size: 2958 1538 33 HaeII r_GCGC'y Cuts at: 1963 2211 3233 4212 1963 Size: 248 1022 979 2280 Fragments arranged by size: 2280 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1129 1161 1182 1214 1417 1449 2371 2401 2403 Size: 32 21 32 203 32 922 30 2 Cuts at: 2403 2433 1129 Size: 30 3225 Fragments arranged by size: 3225 922 203 32 32 32 30 30 21 2 HincII GTy'rAC Cuts at: 1568 1568 Size: 4529 KasI G'GCGC_C Cuts at: 1959 2207 1959 Size: 248 4281 KpnI G_GTAC'C Cuts at: 561 1549 561 Size: 988 3541 MluI A'CGCG_T Cuts at: 3962 3962 Size: 4529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3200 3384 3200 Size: 184 4345 MscI TGG'CCA Cuts at: 799 1060 2290 799 Size: 261 1230 3038 Fragments arranged by size: 3038 1230 261 MslI CAynn'nnrTG Cuts at: 745 1468 2518 2645 745 Size: 723 1050 127 2629 Fragments arranged by size: 2629 1050 723 127 MspA1I CmG'CkG Cuts at: 570 697 1215 1257 1271 1640 1954 2314 3327 Size: 127 518 42 14 369 314 360 1013 Cuts at: 3327 3572 570 Size: 245 1527 Fragments arranged by size: 1527 1013 518 369 360 314 245 127 42 14 NaeI GCC'GGC Cuts at: 2710 2710 Size: 4529 NarI GG'CG_CC Cuts at: 1960 2208 1960 Size: 248 4281 NgoMIV G'CCGG_C Cuts at: 2708 2708 Size: 4529 NspI r_CATG'y Cuts at: 1212 1582 2082 2989 1212 Size: 370 500 907 2752 Fragments arranged by size: 2752 907 500 370 PciI A'CATG_T Cuts at: 2985 2985 Size: 4529 PfoI T'CCnGG_A Cuts at: 1226 1226 Size: 4529 PmeI GTTT'AAAC Cuts at: 1528 1528 Size: 4529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1006 1038 3668 3700 1006 Size: 32 2630 32 1835 Fragments arranged by size: 2630 1835 32 32 PpuMI rG'GwC_Cy Cuts at: 221 618 1506 221 Size: 397 888 3244 Fragments arranged by size: 3244 888 397 PshAI GACnn'nnGTC Cuts at: 593 593 Size: 4529 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1282 1314 1282 Size: 32 4497 PstI C_TGCA'G Cuts at: 121 674 1145 1576 2261 121 Size: 553 471 431 685 2389 Fragments arranged by size: 2389 685 553 471 431 PvuI CG_AT'CG Cuts at: 104 104 Size: 4529 PvuII CAG'CTG Cuts at: 1215 1257 1954 2314 1215 Size: 42 697 360 3430 Fragments arranged by size: 3430 697 360 42 RsrII CG'GwC_CG Cuts at: 2724 2724 Size: 4529 SacI G_AGCT'C Cuts at: 1109 1543 1109 Size: 434 4095 SacII CC_GC'GG Cuts at: 698 698 Size: 4529 SalI G'TCGA_C Cuts at: 1566 1566 Size: 4529 SapI GCTCTTCn'nnn_ Cuts at: 2552 2762 2552 Size: 210 4319 SbfI CC_TGCA'GG Cuts at: 1576 1576 Size: 4529 ScaI AGT'ACT Cuts at: 4046 4046 Size: 4529 SfcI C'TryA_G Cuts at: 33 117 591 670 1141 1572 2257 3250 3441 Size: 84 474 79 471 431 685 993 191 Cuts at: 3441 33 Size: 1121 Fragments arranged by size: 1121 993 685 474 471 431 191 84 79 SfoI GGC'GCC Cuts at: 1961 2209 1961 Size: 248 4281 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4529 SmaI CCC'GGG Cuts at: 1551 1551 Size: 4529 SmlI C'TyrA_G Cuts at: 1684 2599 3091 3353 1684 Size: 915 492 262 2860 Fragments arranged by size: 2860 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4529 SphI G_CATG'C Cuts at: 1212 1582 2082 1212 Size: 370 500 3659 Fragments arranged by size: 3659 500 370 StyI C'CwwG_G Cuts at: 381 1457 1663 2017 381 Size: 1076 206 354 2893 Fragments arranged by size: 2893 1076 354 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 385 889 1510 2112 2245 2704 2913 385 Size: 504 621 602 133 459 209 2001 Fragments arranged by size: 2001 621 602 504 459 209 133 TatI w'GTAC_w Cuts at: 1298 4044 1298 Size: 2746 1783 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1372 1404 4046 4078 1372 Size: 32 2642 32 1823 Fragments arranged by size: 2642 1823 32 32 Tth111I GACn'n_nGTC Cuts at: 2326 2326 Size: 4529 XbaI T'CTAG_A Cuts at: 1560 1560 Size: 4529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 531 807 1071 1125 1234 1402 2024 531 Size: 276 264 54 109 168 622 3036 Fragments arranged by size: 3036 622 276 264 168 109 54 XmaI C'CCGG_G Cuts at: 1549 1549 Size: 4529 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AleI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI