Restriction map for pF1KB5801
 (Linear) (Six-base) MAPSORT of: KIBB5801.seq  Check: 9202  from: 1  to: 1113



KIBB5801.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AflIII A'CryG_T

Cuts at:      0     614    1113 
   Size:        614     499
 
AlwNI CAG_nnn'CTG

Cuts at:      0     992    1113 
   Size:        992     121
 
ApoI r'AATT_y

Cuts at:      0      55     288     336    1096    1113 
   Size:         55     233      48     760      17

  Fragments arranged by size: 

                 760     233      55      48      17
 
AvaI C'yCGr_G

Cuts at:      0     492     562     770    1113 
   Size:        492      70     208     343

  Fragments arranged by size: 

                 492     343     208      70
 
BamHI G'GATC_C

Cuts at:      0     557     942    1113 
   Size:        557     385     171

  Fragments arranged by size: 

                 557     385     171
 
BanII G_rGCy'C

Cuts at:      0     531     802    1113 
   Size:        531     271     311

  Fragments arranged by size: 

                 531     311     271
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     441     640     832    1113 
   Size:        441     199     192     281

  Fragments arranged by size: 

                 441     281     199     192
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     142     176     697     731     959     993    1113 
   Size:        142      34     521      34     228      34     120

  Fragments arranged by size: 

                 521     228     142     120      34      34      34
 
BglII A'GATC_T

Cuts at:      0      27    1113 
   Size:         27    1086
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     715     805    1031    1113 
   Size:        715      90     226      82

  Fragments arranged by size: 

                 715     226      90      82
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     669    1113 
   Size:        669     444
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     465    1113 
   Size:        465     648
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     428     458     516     546    1113 
   Size:        428      30      58      30     567

  Fragments arranged by size: 

                 567     428      58      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     604     607     610     754     757    1113 
   Size:        604       3       3     144       3     356

  Fragments arranged by size: 

                 604     356     144       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     866    1113 
   Size:        866     247
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210    1113 
   Size:        210     903
 
BsiHKAI G_wGCw'C

Cuts at:      0     531    1113 
   Size:        531     582
 
Bsp1286I G_dGCh'C

Cuts at:      0     531     802    1113 
   Size:        531     271     311

  Fragments arranged by size: 

                 531     311     271
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     674    1113 
   Size:        674     439
 
BsrDI GCAATG_nn'

Cuts at:      0     386     954    1113 
   Size:        386     568     159

  Fragments arranged by size: 

                 568     386     159
 
BsrFI r'CCGG_y

Cuts at:      0     995    1113 
   Size:        995     118
 
BssSI C'ACGA_G

Cuts at:      0     144    1113 
   Size:        144     969
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     188     385     992    1113 
   Size:        188     197     607     121

  Fragments arranged by size: 

                 607     197     188     121
 
BstBI TT'CG_AA

Cuts at:      0     903    1113 
   Size:        903     210
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     866     889    1113 
   Size:        866      23     224

  Fragments arranged by size: 

                 866     224      23
 
BstYI r'GATC_y

Cuts at:      0      27     557     942    1113 
   Size:         27     530     385     171

  Fragments arranged by size: 

                 530     385     171      27
 
BtgI C'CryG_G

Cuts at:      0      80     429     584     638    1113 
   Size:         80     349     155      54     475

  Fragments arranged by size: 

                 475     349     155      80      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     232    1113 
   Size:        232     881
 
DraI TTT'AAA

Cuts at:      0     359    1113 
   Size:        359     754
 
EarI CTCTTCn'nnn_

Cuts at:      0     596     914    1113 
   Size:        596     318     199

  Fragments arranged by size: 

                 596     318     199
 
EcoICRI GAG'CTC

Cuts at:      0     529    1113 
   Size:        529     584
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     669    1113 
   Size:        669     444
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     968    1090    1113 
   Size:        968     122      23

  Fragments arranged by size: 

                 968     122      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     176     551     566    1026    1113 
   Size:        176     375      15     460      87

  Fragments arranged by size: 

                 460     375     176      87      15
 
EcoRI G'AATT_C

Cuts at:      0     288    1113 
   Size:        288     825
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      63     400    1113 
   Size:         63     337     713

  Fragments arranged by size: 

                 713     337      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324     428     448     460     480     516     548 
   Size:        292      32     104      20      12      20      36      32

Cuts at:    548    1113 
   Size:        565

  Fragments arranged by size: 

                 565     292     104      36      32      32      20      20
                  12
 
HindIII A'AGCT_T

Cuts at:      0     411    1113 
   Size:        411     702
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     479     728    1113 
   Size:        329     150     249     385

  Fragments arranged by size: 

                 385     329     249     150
 
MslI CAynn'nnrTG

Cuts at:      0     619    1113 
   Size:        619     494
 
NaeI GCC'GGC

Cuts at:      0     997    1113 
   Size:        997     116
 
NcoI C'CATG_G

Cuts at:      0      80     584    1113 
   Size:         80     504     529

  Fragments arranged by size: 

                 529     504      80
 
NgoMIV G'CCGG_C

Cuts at:      0     995    1113 
   Size:        995     118
 
NspI r_CATG'y

Cuts at:      0     618    1072    1113 
   Size:        618     454      41

  Fragments arranged by size: 

                 618     454      41
 
PciI A'CATG_T

Cuts at:      0     614    1113 
   Size:        614     499
 
PfoI T'CCnGG_A

Cuts at:      0     505     553    1113 
   Size:        505      48     560

  Fragments arranged by size: 

                 560     505      48
 
PpuMI rG'GwC_Cy

Cuts at:      0     176     551     566    1026    1113 
   Size:        176     375      15     460      87

  Fragments arranged by size: 

                 460     375     176      87      15
 
PshAI GACnn'nnGTC

Cuts at:      0    1024    1113 
   Size:       1024      89
 
PstI C_TGCA'G

Cuts at:      0     155     763     853     988    1113 
   Size:        155     608      90     135     125

  Fragments arranged by size: 

                 608     155     135     125      90
 
SacI G_AGCT'C

Cuts at:      0     531    1113 
   Size:        531     582
 
SanDI GG'GwC_CC

Cuts at:      0     566    1113 
   Size:        566     547
 
SexAI A'CCwGG_T

Cuts at:      0     541    1113 
   Size:        541     572
 
SfcI C'TryA_G

Cuts at:      0     151     171     759     849     984    1113 
   Size:        151      20     588      90     135     129

  Fragments arranged by size: 

                 588     151     135     129      90      20
 
SmaI CCC'GGG

Cuts at:      0     494    1113 
   Size:        494     619
 
SmlI C'TyrA_G

Cuts at:      0     770    1113 
   Size:        770     343
 
SphI G_CATG'C

Cuts at:      0    1072    1113 
   Size:       1072      41
 
StyI C'CwwG_G

Cuts at:      0      80     584    1113 
   Size:         80     504     529

  Fragments arranged by size: 

                 529     504      80
 
TatI w'GTAC_w

Cuts at:      0     611    1113 
   Size:        611     502
 
XhoI C'TCGA_G

Cuts at:      0     770    1113 
   Size:        770     343
 
XmaI C'CCGG_G

Cuts at:      0     492    1113 
   Size:        492     621
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     AvaI    BamHI    BanII     BbsI     BcgI
    BglII     BmrI     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     DraI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I  HindIII     MmeI     MslI
     NaeI     NcoI   NgoMIV     NspI     PciI     PfoI    PpuMI    PshAI
     PstI     SacI    SanDI    SexAI     SfcI     SmaI     SmlI     SphI
     StyI     TatI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI
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