Restriction map for pF1KB7992
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        757  GGTACC               G^GTACC
AhdI          267  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI           25  GGGCCC               GGGCC^C
Asp718I       757  GGTACC               G^GTACC
AspEI         267  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          278  CCCGGG               C^[CT]CG[GA]G
BanI          757  GGTACC               G^G[CT][GA]CC
BbvCI         321  GCTGAGG              CCTCAGC(-5/-2)
BcgI          301  CGAGTACTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         227  AGATCT               A^GATCT
BsoBI         278  CCCGGG               C^[CT]CG[GA]G
BsrDI         665  CATTGC               GCAATG(2/0)
BsrI          218  CCAGT                ACTGG(1/-1)
BstBI        1048  TTCGAA               TT^CGAA
DsaI          535  CCATGG               C^C[GA][CT]GG
Eam1105I      267  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         390  CCTGCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          417  GCGGG                CCCGC(4/6)
HapII         279  CCGG                 C^CGG
HindIII      1238  AAGCTT               A^AGCTT
HpaII         279  CCGG                 C^CGG
HphI          426  TCACC                GGTGA(8/7)
KpnI          757  GGTACC               GGTAC^C
MspI          279  CCGG                 C^CGG
NcoI          535  CCATGG               C^CATGG
NspV         1048  TTCGAA               TT^CGAA
PleI          267  GACTC                GAGTC(4/5)
PpuMI         497  AGGTCCC              [GA]G^G[AT]CC[CT]
SapI          529  GAAGAGC              GCTCTTC(1/4)
ScaI          303  AGTACT               AGT^ACT
SfuI         1048  TTCGAA               TT^CGAA
SmaI          278  CCCGGG               CCC^GGG
SmlI          288  CTTGAG               C^T[CT][GA]AG
TaqI         1049  TCGA                 T^CGA
TthHB8I      1049  TCGA                 T^CGA
XmaCI         278  CCCGGG               C^CCGGG
XmaI          278  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwI          351  GATCC                GGATC(4/5)
              661  GGATC               
AocI          394  CCTGAGG              CC^T[ACGT]AGG
              595  CCTAAGG             
BcnI          278  CCCGG                CC^[GC]GG
              279  CCGGG               
BfaI           67  CTAG                 C^TAG
               77  CTAG                
BsaI          190  GAGACC               GGTCTC(1/5)
              601  GAGACC              
BseRI         397  GAGGAG               GAGGAG(10/8)
              442  GAGGAG              
BspMI         519  ACCTGC               ACCTGC(4/8)
              495  GCAGGT              
BstYI         227  AGATCT               [GA]^GATC[CT]
              350  AGATCC              
Bsu36I        394  CCTGAGG              CC^T[ACGT]AGG
              595  CCTAAGG             
EarI          166  GAAGAG               CTCTTC(1/4)
              529  GAAGAG              
Eco81I        394  CCTGAGG              CC^T[ACGT]AGG
              595  CCTAAGG             
EcoRI        1025  GAATTC               G^AATTC
             1193  GAATTC              
EcoT14I       535  CCATGG               C^C[AT][AT]GG
              650  CCAAGG              
Ksp632I       166  GAAGAG               CTCTTC(1/4)
              529  GAAGAG              
MaeI           67  CTAG                 C^TAG
               77  CTAG                
MflI          227  AGATCT               [GA]^GATC[CT]
              350  AGATCC              
MspA1I         42  CAGCTG               C[AC]G^C[GT]G
              101  CAGCTG              
MwoI          393  GCCTGAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              565  GCCCAGGAAGC         
NciI          278  CCCGG                CC^[GC]GG
              279  CCGGG               
PstI           59  CTGCAG               CTGCA^G
              769  CTGCAG              
PvuII          42  CAGCTG               CAG^CTG
              101  CAGCTG              
SfcI           59  CTGCAG               C^T[GA][CT]AG
              769  CTGCAG              
StyI          535  CCATGG               C^C[AT][AT]GG
              650  CCAAGG              
Tsp45I        451  GTGAC                ^GT[GC]AC
              974  GTGAC               
TspRI         575  CACTG                CA[GC]TG[ACGT][ACGT]^
             1200  CAGTG               
XcmI          261  CCAGGAGACTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              680  CCATTACTGAAGTGG     
XhoII         227  AGATCT               [GA]^GATC[CT]
              350  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        Asp700I     AspHI       AvaII       BanII       
BbvI        BmyI        BpmI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeIII      MboI        MboII       MnlI        MvaI        
NdeII       NlaIII      NlaIV       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       StuI        
TfiI        TseI        Tsp509I     XmnI        
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