Restriction map for
pF1KB7992
(Circular) (Six-base) MAPSORT of: pF1KB7992.seq Check: 6188 from: 1 to: 4418
pF1KB7992 4418 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1456 1456
Size: 4418
Acc65I G'GTAC_C
Cuts at: 865 1434 865
Size: 569 3849
AclI AA'CG_TT
Cuts at: 4258 4258
Size: 4418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 336 815 1167 2243 2675 3422 3690 211
Size: 125 479 352 1076 432 747 268 939
Fragments arranged by size:
1076 939 747 479 432 352 268 125
AflIII A'CryG_T
Cuts at: 1770 2874 3851 1770
Size: 1104 977 2337
Fragments arranged by size:
2337 1104 977
AgeI A'CCGG_T
Cuts at: 2806 2806
Size: 4418
AhdI GACnn_n'nnGTC
Cuts at: 380 380
Size: 4418
AlwNI CAG_nnn'CTG
Cuts at: 211 462 616 1723 3290 211
Size: 251 154 1107 1567 1339
Fragments arranged by size:
1567 1339 1107 251 154
ApaI G_GGCC'C
Cuts at: 137 137
Size: 4418
ApaLI G'TGCA_C
Cuts at: 3188 3188
Size: 4418
ApoI r'AATT_y
Cuts at: 965 1133 1301 1422 3771 4027 965
Size: 168 168 121 2349 256 1356
Fragments arranged by size:
2349 1356 256 168 168 121
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 386 1438 386
Size: 1052 3366
BamHI G'GATC_C
Cuts at: 1443 1443
Size: 4418
BanI G'GyrC_C
Cuts at: 865 1434 1848 2096 2131 865
Size: 569 414 248 35 3152
Fragments arranged by size:
3152 569 414 248 35
BanII G_rGCy'C
Cuts at: 137 512 758 1111 1370 1432 2462 137
Size: 375 246 353 259 62 1030 2093
Fragments arranged by size:
2093 1030 375 353 259 246 62
BbeI G_GCGC'C
Cuts at: 1852 2100 1852
Size: 248 4170
BbvCI CC'TCA_GC
Cuts at: 430 430
Size: 4418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 398 432 398
Size: 34 4384
BciVI GTATCCnnnnn_n'
Cuts at: 2316 3077 4418 2316
Size: 761 1341 2316
Fragments arranged by size:
2316 1341 761
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BglII A'GATC_T
Cuts at: 335 1933 3970 335
Size: 1598 2037 783
Fragments arranged by size:
2037 1598 783
BlpI GC'TnA_GC
Cuts at: 1530 1530
Size: 4418
Bme1580I G_kGCm'C
Cuts at: 137 2043 2136 3192 137
Size: 1906 93 1056 1363
Fragments arranged by size:
1906 1363 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1630 1711 2046 4190 1630
Size: 81 335 2144 1858
Fragments arranged by size:
2144 1858 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 589 682 1079 484
Size: 105 93 397 3823
Fragments arranged by size:
3823 397 105 93
Bpu10I CC'TnA_GC
Cuts at: 430 430
Size: 4418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 1594 2473 2965 3263 417
Size: 1177 879 492 298 1572
Fragments arranged by size:
1572 1177 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 292 703 4122 292
Size: 411 3419 588
Fragments arranged by size:
3419 588 411
BsaAI yAC'GTr
Cuts at: 1771 1771
Size: 4418
BsaBI GATnn'nnATC
Cuts at: 6 1932 3969 6
Size: 1926 2037 455
Fragments arranged by size:
2037 1926 455
BsaHI Gr'CG_yC
Cuts at: 1849 2097 2799 4295 1849
Size: 248 702 1496 1972
Fragments arranged by size:
1972 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1607 2128 2806 3080 3227 3638 1607
Size: 521 678 274 147 411 2387
Fragments arranged by size:
2387 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 565 520
Size: 45 4373
BseYI C'CCAG_C
Cuts at: 713 1843 2601 3178 3674 713
Size: 1130 758 577 496 1457
Fragments arranged by size:
1457 1130 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 602 614 199
Size: 403 12 4003
Fragments arranged by size:
4003 403 12
BsiEI CG_ry'CG
Cuts at: 104 1960 2006 3214 104
Size: 1856 46 1208 1308
Fragments arranged by size:
1856 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 512 1111 1197 1363 1370 1432 2210 2400 3192
Size: 599 86 166 7 62 778 190 792
Cuts at: 3192 512
Size: 1738
Fragments arranged by size:
1738 792 778 599 190 166 86 62
7
Bsp1286I G_dGCh'C
Cuts at: 137 512 758 1111 1197 1363 1370 1432 2043
Size: 375 246 353 86 166 7 62 611
Cuts at: 2043 2136 2210 2400 2462 3192 137
Size: 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 375 353 246 190 166
93 86 74 62 62 7
BspEI T'CCGG_A
Cuts at: 3638 3638
Size: 4418
BspHI T'CATG_A
Cuts at: 3594 3594
Size: 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 594 636 1468 1682 1984 2365 594
Size: 42 832 214 302 381 2647
Fragments arranged by size:
2647 832 381 302 214 42
BsrBI CCG'CTC
Cuts at: 50 2710 2764 4248 4413 50
Size: 2660 54 1484 165 55
Fragments arranged by size:
2660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 772 2330 772
Size: 1558 2860
BsrFI r'CCGG_y
Cuts at: 2416 2597 2806 2416
Size: 181 209 4028
Fragments arranged by size:
4028 209 181
BssSI C'ACGA_G
Cuts at: 2689 3047 2689
Size: 358 4060
BstAPI GCAn_nnn'nTGC
Cuts at: 1795 1795
Size: 4418
BstBI TT'CG_AA
Cuts at: 1157 2779 1157
Size: 1622 2796
BstYI r'GATC_y
Cuts at: 1 335 458 1443 1910 1933 2268 2514 3526
Size: 334 123 985 467 23 335 246 1012
Cuts at: 3526 3612 3624 3970 1
Size: 86 12 346 449
Fragments arranged by size:
1012 985 467 449 346 335 334 246
123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 503 704 503
Size: 201 4217
BtgI C'CryG_G
Cuts at: 107 643 107
Size: 536 3882
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2368 2549 2712 2368
Size: 181 163 4074
Fragments arranged by size:
4074 181 163
BtsI GCAGTG_nn'
Cuts at: 1661 1661
Size: 4418
DraI TTT'AAA
Cuts at: 1417 1417
Size: 4418
DrdI GACnn_nn'nnGTC
Cuts at: 2124 2982 2124
Size: 858 3560
EaeI y'GGCC_r
Cuts at: 2003 2177 2568 2595 3940 2003
Size: 174 391 27 1345 2481
Fragments arranged by size:
2481 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2003 2003
Size: 4418
EarI CTCTTCn'nnn_
Cuts at: 269 632 2441 2651 269
Size: 363 1809 210 2036
Fragments arranged by size:
2036 1809 363 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 331 2626 2936 3082 4228 331
Size: 2295 310 146 1146 521
Fragments arranged by size:
2295 1146 521 310 146
EcoICRI GAG'CTC
Cuts at: 510 1109 1368 1430 510
Size: 599 259 62 3498
Fragments arranged by size:
3498 599 259 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 336 484 589 682 815 1079 1167 2243
Size: 125 148 105 93 133 264 88 1076
Cuts at: 2243 2675 3422 3690 211
Size: 432 747 268 939
Fragments arranged by size:
1076 939 747 432 268 264 148 133
125 105 93 88
EcoNI CCTnn'n_nnAGG
Cuts at: 502 502
Size: 4418
EcoO109I rG'GnC_Cy
Cuts at: 133 134 606 613 1557 133
Size: 1 472 7 944 2994
Fragments arranged by size:
2994 944 472 7 1
EcoRI G'AATT_C
Cuts at: 1133 1301 1422 1133
Size: 168 121 4129
Fragments arranged by size:
4129 168 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4418
FspI TGC'GCA
Cuts at: 2199 2199
Size: 4418
HaeII r_GCGC'y
Cuts at: 1852 2100 3122 4101 1852
Size: 248 1022 979 2169
Fragments arranged by size:
2169 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 366 398 499 531 754 786 2260
Size: 32 66 32 101 32 223 32 1474
Cuts at: 2260 2290 2292 2322 268
Size: 30 2 30 2364
Fragments arranged by size:
2364 1474 223 101 66 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1457 1457
Size: 4418
HindIII A'AGCT_T
Cuts at: 1346 1346
Size: 4418
KasI G'GCGC_C
Cuts at: 1848 2096 1848
Size: 248 4170
KpnI G_GTAC'C
Cuts at: 869 1438 869
Size: 569 3849
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3089 3273 3089
Size: 184 4234
MscI TGG'CCA
Cuts at: 2179 2179
Size: 4418
MslI CAynn'nnrTG
Cuts at: 2407 2534 2407
Size: 127 4291
MspA1I CmG'CkG
Cuts at: 152 211 1529 1843 2203 3216 3461 152
Size: 59 1318 314 360 1013 245 1109
Fragments arranged by size:
1318 1109 1013 360 314 245 59
NaeI GCC'GGC
Cuts at: 2599 2599
Size: 4418
NarI GG'CG_CC
Cuts at: 1849 2097 1849
Size: 248 4170
NcoI C'CATG_G
Cuts at: 107 643 107
Size: 536 3882
NgoMIV G'CCGG_C
Cuts at: 2597 2597
Size: 4418
NspI r_CATG'y
Cuts at: 1471 1971 2878 1471
Size: 500 907 3011
Fragments arranged by size:
3011 907 500
PciI A'CATG_T
Cuts at: 2874 2874
Size: 4418
PfoI T'CCnGG_A
Cuts at: 461 461
Size: 4418
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4386
PpuMI rG'GwC_Cy
Cuts at: 606 606
Size: 4418
PsiI TTA'TAA
Cuts at: 1022 1022
Size: 4418
PspOMI G'GGCC_C
Cuts at: 133 133
Size: 4418
PstI C_TGCA'G
Cuts at: 171 881 1465 2150 171
Size: 710 584 685 2439
Fragments arranged by size:
2439 710 685 584
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 152 211 1843 2203 152
Size: 59 1632 360 2367
Fragments arranged by size:
2367 1632 360 59
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 512 1111 1370 1432 512
Size: 599 259 62 3498
Fragments arranged by size:
3498 599 259 62
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SapI GCTCTTCn'nnn_
Cuts at: 632 2441 2651 632
Size: 1809 210 2399
Fragments arranged by size:
2399 1809 210
SbfI CC_TGCA'GG
Cuts at: 1465 1465
Size: 4418
ScaI AGT'ACT
Cuts at: 413 3935 413
Size: 3522 896
SfcI C'TryA_G
Cuts at: 33 167 877 1461 2146 3139 3330 33
Size: 134 710 584 685 993 191 1121
Fragments arranged by size:
1121 993 710 685 584 191 134
SfoI GGC'GCC
Cuts at: 1850 2098 1850
Size: 248 4170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SmaI CCC'GGG
Cuts at: 388 1440 388
Size: 1052 3366
SmlI C'TyrA_G
Cuts at: 396 1573 2488 2980 3242 396
Size: 1177 915 492 262 1572
Fragments arranged by size:
1572 1177 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 1471 1971 1471
Size: 500 3918
StuI AGG'CCT
Cuts at: 394 1180 1264 394
Size: 786 84 3548
Fragments arranged by size:
3548 786 84
StyI C'CwwG_G
Cuts at: 107 643 758 1552 1906 107
Size: 536 115 794 354 2619
Fragments arranged by size:
2619 794 536 354 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 510 801 1108 2001 2134 2593 2802 510
Size: 291 307 893 133 459 209 2126
Fragments arranged by size:
2126 893 459 307 291 209 133
TatI w'GTAC_w
Cuts at: 411 3933 411
Size: 3522 896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 674 706 3935 3967 674
Size: 32 3229 32 1125
Fragments arranged by size:
3229 1125 32 32
Tth111I GACn'n_nGTC
Cuts at: 2215 2215
Size: 4418
XbaI T'CTAG_A
Cuts at: 1449 1449
Size: 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 376 795 1913 376
Size: 419 1118 2881
Fragments arranged by size:
2881 1118 419
XmaI C'CCGG_G
Cuts at: 386 1438 386
Size: 1052 3366
XmnI GAAnn'nnTTC
Cuts at: 1096 1153 1180 1096
Size: 57 27 4334
Fragments arranged by size:
4334 57 27
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI