Restriction map for
pF1KB9934
(Linear) (Six-base) MAPSORT of: KIBB9934.seq Check: 3736 from: 1 to: 1941
KIBB9934.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 1189 1941
Size: 594 595 752
Fragments arranged by size:
752 595 594
AfeI AGC'GCT
Cuts at: 0 268 1941
Size: 268 1673
AflIII A'CryG_T
Cuts at: 0 327 1941
Size: 327 1614
AgeI A'CCGG_T
Cuts at: 0 642 1941
Size: 642 1299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 1941
Size: 947 994
AlwNI CAG_nnn'CTG
Cuts at: 0 123 384 549 681 1377 1398 1941
Size: 123 261 165 132 696 21 543
Fragments arranged by size:
696 543 261 165 132 123 21
ApaI G_GGCC'C
Cuts at: 0 155 1941
Size: 155 1786
AvaI C'yCGr_G
Cuts at: 0 562 1297 1941
Size: 562 735 644
Fragments arranged by size:
735 644 562
BanI G'GyrC_C
Cuts at: 0 808 1016 1201 1222 1312 1797 1941
Size: 808 208 185 21 90 485 144
Fragments arranged by size:
808 485 208 185 144 90 21
BanII G_rGCy'C
Cuts at: 0 155 287 370 1337 1453 1517 1941
Size: 155 132 83 967 116 64 424
Fragments arranged by size:
967 424 155 132 116 83 64
BbeI G_GCGC'C
Cuts at: 0 1226 1941
Size: 1226 715
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 1941
Size: 50 1891
BciVI GTATCCnnnnn_n'
Cuts at: 0 1825 1941
Size: 1825 116
BglI GCCn_nnn'nGGC
Cuts at: 0 100 799 1941
Size: 100 699 1142
Fragments arranged by size:
1142 699 100
BglII A'GATC_T
Cuts at: 0 1127 1941
Size: 1127 814
BlpI GC'TnA_GC
Cuts at: 0 518 949 1941
Size: 518 431 992
Fragments arranged by size:
992 518 431
Bme1580I G_kGCm'C
Cuts at: 0 155 813 1802 1941
Size: 155 658 989 139
Fragments arranged by size:
989 658 155 139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 912 1467 1941
Size: 912 555 474
Fragments arranged by size:
912 555 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 721 1290 1441 1462 1759 1894 1941
Size: 49 672 569 151 21 297 135 47
Fragments arranged by size:
672 569 297 151 135 49 47 21
Bpu10I CC'TnA_GC
Cuts at: 0 1290 1941
Size: 1290 651
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 442 545 821 1077 1316 1941
Size: 25 417 103 276 256 239 625
Fragments arranged by size:
625 417 276 256 239 103 25
BsaAI yAC'GTr
Cuts at: 0 474 1491 1905 1941
Size: 474 1017 414 36
Fragments arranged by size:
1017 474 414 36
BsaHI Gr'CG_yC
Cuts at: 0 1223 1941
Size: 1223 718
BsaWI w'CCGG_w
Cuts at: 0 642 1343 1817 1941
Size: 642 701 474 124
Fragments arranged by size:
701 642 474 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 487 517 1941
Size: 22 30 435 30 1424
Fragments arranged by size:
1424 435 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 76 1606 1609 1639 1891 1894 1912
Size: 18 58 1530 3 30 252 3 18
Cuts at: 1912 1941
Size: 29
Fragments arranged by size:
1530 252 58 30 29 18 18 3
3
BseYI C'CCAG_C
Cuts at: 0 116 634 663 1484 1518 1733 1941
Size: 116 518 29 821 34 215 208
Fragments arranged by size:
821 518 215 208 116 34 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 426 1652 1927 1941
Size: 6 420 1226 275 14
Fragments arranged by size:
1226 420 275 14 6
BsiEI CG_ry'CG
Cuts at: 0 107 323 1941
Size: 107 216 1618
Fragments arranged by size:
1618 216 107
BsiHKAI G_wGCw'C
Cuts at: 0 370 1337 1453 1904 1941
Size: 370 967 116 451 37
Fragments arranged by size:
967 451 370 116 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1714 1941
Size: 1714 227
Bsp1286I G_dGCh'C
Cuts at: 0 155 287 370 813 1337 1453 1517 1802
Size: 155 132 83 443 524 116 64 285
Cuts at: 1802 1904 1941
Size: 102 37
Fragments arranged by size:
524 443 285 155 132 116 102 83
64 37
BspEI T'CCGG_A
Cuts at: 0 1343 1817 1941
Size: 1343 474 124
Fragments arranged by size:
1343 474 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 1189 1941
Size: 594 595 752
Fragments arranged by size:
752 595 594
BsrBI CCG'CTC
Cuts at: 0 8 72 281 522 1629 1941
Size: 8 64 209 241 1107 312
Fragments arranged by size:
1107 312 241 209 64 8
BsrDI GCAATG_nn'
Cuts at: 0 1244 1941
Size: 1244 697
BsrFI r'CCGG_y
Cuts at: 0 642 793 830 1941
Size: 642 151 37 1111
Fragments arranged by size:
1111 642 151 37
BssSI C'ACGA_G
Cuts at: 0 88 1941
Size: 88 1853
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1695 1941
Size: 1695 246
BstYI r'GATC_y
Cuts at: 0 485 1127 1941
Size: 485 642 814
Fragments arranged by size:
814 642 485
Bsu36I CC'TnA_GG
Cuts at: 0 170 1941
Size: 170 1771
BtgI C'CryG_G
Cuts at: 0 293 447 1326 1469 1802 1941
Size: 293 154 879 143 333 139
Fragments arranged by size:
879 333 293 154 143 139
BtsI GCAGTG_nn'
Cuts at: 0 1587 1941
Size: 1587 354
DraIII CAC_nnn'GTG
Cuts at: 0 474 1941
Size: 474 1467
DrdI GACnn_nn'nnGTC
Cuts at: 0 1774 1941
Size: 1774 167
EaeI y'GGCC_r
Cuts at: 0 104 156 320 608 651 756 800 1458
Size: 104 52 164 288 43 105 44 658
Cuts at: 1458 1676 1941
Size: 218 265
Fragments arranged by size:
658 288 265 218 164 105 104 52
44 43
EagI C'GGCC_G
Cuts at: 0 104 320 1941
Size: 104 216 1621
Fragments arranged by size:
1621 216 104
EarI CTCTTCn'nnn_
Cuts at: 0 220 1441 1941
Size: 220 1221 500
Fragments arranged by size:
1221 500 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 102 202 1941
Size: 102 100 1739
Fragments arranged by size:
1739 102 100
EcoICRI GAG'CTC
Cuts at: 0 368 1335 1451 1941
Size: 368 967 116 490
Fragments arranged by size:
967 490 368 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 721 1290 1441 1462 1759 1894 1941
Size: 49 672 569 151 21 297 135 47
Fragments arranged by size:
672 569 297 151 135 49 47 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 802 925 1196 1941
Size: 802 123 271 745
Fragments arranged by size:
802 745 271 123
EcoO109I rG'GnC_Cy
Cuts at: 0 546 744 891 939 999 1294 1301 1941
Size: 546 198 147 48 60 295 7 640
Fragments arranged by size:
640 546 295 198 147 60 48 7
EcoRV GAT'ATC
Cuts at: 0 1920 1941
Size: 1920 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 1941
Size: 522 1419
HaeII r_GCGC'y
Cuts at: 0 78 270 974 1226 1941
Size: 78 192 704 252 715
Fragments arranged by size:
715 704 252 192 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1250 1282 1338 1370 1941
Size: 1250 32 56 32 571
Fragments arranged by size:
1250 571 56 32 32
HincII GTy'rAC
Cuts at: 0 495 1941
Size: 495 1446
KasI G'GCGC_C
Cuts at: 0 1222 1941
Size: 1222 719
MscI TGG'CCA
Cuts at: 0 653 1460 1678 1941
Size: 653 807 218 263
Fragments arranged by size:
807 653 263 218
MslI CAynn'nnrTG
Cuts at: 0 56 1941
Size: 56 1885
MspA1I CmG'CkG
Cuts at: 0 123 295 663 1310 1319 1617 1737 1764
Size: 123 172 368 647 9 298 120 27
Cuts at: 1764 1941
Size: 177
Fragments arranged by size:
647 368 298 177 172 123 120 27
9
NaeI GCC'GGC
Cuts at: 0 795 1941
Size: 795 1146
NarI GG'CG_CC
Cuts at: 0 1223 1941
Size: 1223 718
NcoI C'CATG_G
Cuts at: 0 447 1941
Size: 447 1494
NgoMIV G'CCGG_C
Cuts at: 0 793 1941
Size: 793 1148
NotI GC'GGCC_GC
Cuts at: 0 104 1941
Size: 104 1837
PasI CC'CwG_GG
Cuts at: 0 766 1941
Size: 766 1175
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 606 1941
Size: 384 222 1335
Fragments arranged by size:
1335 384 222
PmlI CAC'GTG
Cuts at: 0 474 1905 1941
Size: 474 1431 36
Fragments arranged by size:
1431 474 36
PspOMI G'GGCC_C
Cuts at: 0 151 1941
Size: 151 1790
PstI C_TGCA'G
Cuts at: 0 254 677 926 1403 1941
Size: 254 423 249 477 538
Fragments arranged by size:
538 477 423 254 249
PvuII CAG'CTG
Cuts at: 0 123 1617 1737 1764 1941
Size: 123 1494 120 27 177
Fragments arranged by size:
1494 177 123 120 27
SacI G_AGCT'C
Cuts at: 0 370 1337 1453 1941
Size: 370 967 116 488
Fragments arranged by size:
967 488 370 116
SacII CC_GC'GG
Cuts at: 0 296 1941
Size: 296 1645
SapI GCTCTTCn'nnn_
Cuts at: 0 220 1441 1941
Size: 220 1221 500
Fragments arranged by size:
1221 500 220
SbfI CC_TGCA'GG
Cuts at: 0 677 1941
Size: 677 1264
SexAI A'CCwGG_T
Cuts at: 0 455 1941
Size: 455 1486
SfcI C'TryA_G
Cuts at: 0 250 673 922 1399 1825 1941
Size: 250 423 249 477 426 116
Fragments arranged by size:
477 426 423 250 249 116
SfoI GGC'GCC
Cuts at: 0 1224 1941
Size: 1224 717
SmaI CCC'GGG
Cuts at: 0 1299 1941
Size: 1299 642
StuI AGG'CCT
Cuts at: 0 16 1744 1941
Size: 16 1728 197
Fragments arranged by size:
1728 197 16
StyI C'CwwG_G
Cuts at: 0 422 430 447 1708 1941
Size: 422 8 17 1261 233
Fragments arranged by size:
1261 422 233 17 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 1046 1941
Size: 460 586 895
Fragments arranged by size:
895 586 460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 443 475 1941
Size: 22 32 389 32 1466
Fragments arranged by size:
1466 389 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 0 1049 1941
Size: 1049 892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1095 1359 1378 1416 1506 1941
Size: 1095 264 19 38 90 435
Fragments arranged by size:
1095 435 264 90 38 19
XmaI C'CCGG_G
Cuts at: 0 1297 1941
Size: 1297 644
Enzymes that do cut:
AarI AfeI AflIII AgeI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BciVI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI PasI PflMI PmlI PspOMI PstI
PvuII SacI SacII SapI SbfI SexAI SfcI SfoI
SmaI StuI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BmgBI BmrI BmtI
BpuEI BsaBI BsiWI BsmI BspHI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EcoRI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NruI NsiI NspI PacI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI ScaI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI XmnI ZraI