################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccII 93 CGCG CG^CG AlwNI 832 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 513 GGTGCC G^G[CT][GA]CC BpmI 874 CTCCAG CTGGAG(16/14) BsaI 179 GAGACC GGTCTC(1/5) BsmBI 887 GAGACG CGTCTC(1/5) BsrBI 283 GAGCGG CCGCTC(-3/-3) BsrFI 257 GCCGGT [GA]^CCGG[CT] BstAPI 897 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 381 GGTGACC G^GT[ACGT]ACC BstPI 381 GGTGACC G^GT[ACGT]ACC BstUI 93 CGCG CG^CG Cfr10I 257 GCCGGT [GA]^CCGG[CT] EaeI 255 TGGCCG [CT]^GGCC[GA] EcoO65I 381 GGTGACC G^GT[ACGT]ACC HaeII 856 GGCGCT [GA]GCGC^[CT] MaeII 899 ACGT A^CGT MseI 569 TTAA T^TAA MslI 684 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 965 CAGCTG C[AC]G^C[GT]G MvnI 93 CGCG CG^CG PstI 482 CTGCAG CTGCA^G PvuII 965 CAGCTG CAG^CTG SacI 820 GAGCTC GAGCT^C SfaNI 592 GCATC GCATC(5/9) SfcI 482 CTGCAG C^T[GA][CT]AG SmaI 239 CCCGGG CCC^GGG Tru9I 569 TTAA T^TAA Tsp509I 506 AATT ^AATT XmaCI 239 CCCGGG C^CCGGG XmaI 239 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Alw44I 322 GTGCAC G^TGCAC 895 GTGCAC ApaI 242 GGGCCC GGGCC^C 787 GGGCCC ApaLI 322 GTGCAC G^TGCAC 895 GTGCAC BglI 544 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 750 GCCAGGAGGGC BlpI 628 GCTGAGC GC^T[ACGT]AGC 694 GCTGAGC Bpu1102I 628 GCTGAGC GC^T[ACGT]AGC 694 GCTGAGC BsgI 869 CTGCAC GTGCAG(16/14) 1003 CTGCAC BspMI 305 ACCTGC ACCTGC(4/8) 330 ACCTGC BsrI 517 CCAGT ACTGG(1/-1) 920 CCAGT BstXI 246 CCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 683 CCACCCATGTGG BstYI 558 GGATCT [GA]^GATC[CT] 992 AGATCC CelII 628 GCTGAGC GC^T[ACGT]AGC 694 GCTGAGC MflI 558 GGATCT [GA]^GATC[CT] 992 AGATCC NlaIII 175 CATG CATG^ 688 CATG PaeR7I 217 CTCGAG C^TCGAG 499 CTCGAG Tsp45I 382 GTGAC ^GT[GC]AC 923 GTGAC XhoI 217 CTCGAG C^TCGAG 499 CTCGAG XhoII 558 GGATCT [GA]^GATC[CT] 992 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SmlI StyI TaqI TseI TspRI TthHB8I