(Circular) (Six-base) MAPSORT of: pF1KB9923.seq Check: 9711 from: 1 to: 4139 pF1KB9923 4139 bp With 182 enzymes: SgfI * October 7, 2009 14:26 .. AccI GT'mk_AC Cuts at: 1177 1177 Size: 4139 Acc65I G'GTAC_C Cuts at: 1155 1155 Size: 4139 AclI AA'CG_TT Cuts at: 3979 3979 Size: 4139 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1000 1105 1964 2396 3143 3411 1000 Size: 105 859 432 747 268 1728 Fragments arranged by size: 1728 859 747 432 268 105 AflIII A'CryG_T Cuts at: 1491 2595 3572 1491 Size: 1104 977 2058 Fragments arranged by size: 2058 1104 977 AgeI A'CCGG_T Cuts at: 2527 2527 Size: 4139 AleI CACnn'nnGTG Cuts at: 796 796 Size: 4139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 455 455 Size: 4139 AlwNI CAG_nnn'CTG Cuts at: 945 1444 3011 945 Size: 499 1567 2073 Fragments arranged by size: 2073 1567 499 ApaI G_GGCC'C Cuts at: 354 899 354 Size: 545 3594 ApaLI G'TGCA_C Cuts at: 430 1003 2909 430 Size: 573 1906 1660 Fragments arranged by size: 1906 1660 573 ApoI r'AATT_y Cuts at: 1143 3492 3748 1143 Size: 2349 256 1534 Fragments arranged by size: 2349 1534 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4139 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4139 AvaI C'yCGr_G Cuts at: 325 347 607 716 1159 325 Size: 22 260 109 443 3305 Fragments arranged by size: 3305 443 260 109 22 BamHI G'GATC_C Cuts at: 1164 1164 Size: 4139 BanI G'GyrC_C Cuts at: 621 1155 1569 1817 1852 621 Size: 534 414 248 35 2908 Fragments arranged by size: 2908 534 414 248 35 BanII G_rGCy'C Cuts at: 354 899 932 1153 2183 354 Size: 545 33 221 1030 2310 Fragments arranged by size: 2310 1030 545 221 33 BbeI G_GCGC'C Cuts at: 1573 1821 1573 Size: 248 3891 BciVI GTATCCnnnnn_n' Cuts at: 2037 2798 4139 2037 Size: 761 1341 2037 Fragments arranged by size: 2037 1341 761 BclI T'GATC_A Cuts at: 1659 1659 Size: 4139 BglI GCCn_nnn'nGGC Cuts at: 658 864 658 Size: 206 3933 BglII A'GATC_T Cuts at: 1654 3691 1654 Size: 2037 2102 BlpI GC'TnA_GC Cuts at: 737 803 1251 737 Size: 66 448 3625 Fragments arranged by size: 3625 448 66 Bme1580I G_kGCm'C Cuts at: 354 434 899 1007 1764 1857 2913 354 Size: 80 465 108 757 93 1056 1580 Fragments arranged by size: 1580 1056 757 465 108 93 80 BmgBI CAC'GTC Cuts at: 1008 1008 Size: 4139 BmrI ACTGGGnnnn_n' Cuts at: 1022 1351 1432 1767 3911 1022 Size: 329 81 335 2144 1250 Fragments arranged by size: 2144 1250 335 329 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 742 2506 742 Size: 1764 2375 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 967 967 Size: 4139 Bpu10I CC'TnA_GC Cuts at: 913 913 Size: 4139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 448 1315 2194 2686 2984 448 Size: 867 879 492 298 1603 Fragments arranged by size: 1603 879 867 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 281 3843 281 Size: 3562 577 BsaAI yAC'GTr Cuts at: 1492 1492 Size: 4139 BsaBI GATnn'nnATC Cuts at: 6 1653 3690 6 Size: 1647 2037 455 Fragments arranged by size: 2037 1647 455 BsaHI Gr'CG_yC Cuts at: 1570 1818 2520 4016 1570 Size: 248 702 1496 1693 Fragments arranged by size: 1693 1496 702 248 BsaWI w'CCGG_w Cuts at: 1328 1849 2527 2801 2948 3359 1328 Size: 521 678 274 147 411 2108 Fragments arranged by size: 2108 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 745 775 811 841 745 Size: 30 36 30 4043 Fragments arranged by size: 4043 36 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 343 403 661 709 886 1111 343 Size: 60 258 48 177 225 3371 Fragments arranged by size: 3371 258 225 177 60 48 BseYI C'CCAG_C Cuts at: 458 784 1564 2322 2899 3395 458 Size: 326 780 758 577 496 1202 Fragments arranged by size: 1202 780 758 577 496 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 962 1096 962 Size: 134 4005 BsiEI CG_ry'CG Cuts at: 104 1681 1727 2935 104 Size: 1577 46 1208 1308 Fragments arranged by size: 1577 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 434 691 932 1007 1153 1931 2121 2913 434 Size: 257 241 75 146 778 190 792 1660 Fragments arranged by size: 1660 792 778 257 241 190 146 75 BsmBI CGTCTCn'nnnn_ Cuts at: 989 989 Size: 4139 Bsp1286I G_dGCh'C Cuts at: 354 434 691 899 932 1007 1153 1764 1857 Size: 80 257 208 33 75 146 611 93 Cuts at: 1857 1931 2121 2183 2913 354 Size: 74 190 62 730 1580 Fragments arranged by size: 1580 730 611 257 208 190 146 93 80 75 74 62 33 BspEI T'CCGG_A Cuts at: 3359 3359 Size: 4139 BspHI T'CATG_A Cuts at: 3315 3315 Size: 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 422 447 1189 1403 1705 2086 422 Size: 25 742 214 302 381 2475 Fragments arranged by size: 2475 742 381 302 214 25 BsrBI CCG'CTC Cuts at: 50 393 2431 2485 3969 4134 50 Size: 343 2038 54 1484 165 55 Fragments arranged by size: 2038 1484 343 165 55 54 BsrDI GCAATG_nn' Cuts at: 2051 2051 Size: 4139 BsrFI r'CCGG_y Cuts at: 365 2137 2318 2527 365 Size: 1772 181 209 1977 Fragments arranged by size: 1977 1772 209 181 BssSI C'ACGA_G Cuts at: 2410 2768 2410 Size: 358 3781 BstAPI GCAn_nnn'nTGC Cuts at: 1011 1516 1011 Size: 505 3634 BstBI TT'CG_AA Cuts at: 2500 2500 Size: 4139 BstEII G'GTnAC_C Cuts at: 489 489 Size: 4139 BstXI CCAn_nnnn'nTGG Cuts at: 361 798 361 Size: 437 3702 BstYI r'GATC_y Cuts at: 1 666 1100 1164 1631 1654 1989 2235 3247 Size: 665 434 64 467 23 335 246 1012 Cuts at: 3247 3333 3345 3691 1 Size: 86 12 346 449 Fragments arranged by size: 1012 665 467 449 434 346 335 246 86 64 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2089 2270 2433 2089 Size: 181 163 3795 Fragments arranged by size: 3795 181 163 BtsI GCAGTG_nn' Cuts at: 1382 1382 Size: 4139 DraI TTT'AAA Cuts at: 1138 1138 Size: 4139 DrdI GACnn_nn'nnGTC Cuts at: 1845 2703 1845 Size: 858 3281 EaeI y'GGCC_r Cuts at: 363 1724 1898 2289 2316 3661 363 Size: 1361 174 391 27 1345 841 Fragments arranged by size: 1361 1345 841 391 174 27 EagI C'GGCC_G Cuts at: 1724 1724 Size: 4139 EarI CTCTTCn'nnn_ Cuts at: 319 754 872 2162 2372 319 Size: 435 118 1290 210 2086 Fragments arranged by size: 2086 1290 435 210 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 307 2347 2657 2803 3949 307 Size: 2040 310 146 1146 497 Fragments arranged by size: 2040 1146 497 310 146 EcoICRI GAG'CTC Cuts at: 930 1151 930 Size: 221 3918 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 967 1000 1105 1964 2396 3143 3411 967 Size: 33 105 859 432 747 268 1695 Fragments arranged by size: 1695 859 747 432 268 105 33 EcoNI CCTnn'n_nnAGG Cuts at: 716 730 916 716 Size: 14 186 3939 Fragments arranged by size: 3939 186 14 EcoO109I rG'GnC_Cy Cuts at: 222 411 516 603 660 721 895 942 1278 Size: 189 105 87 57 61 174 47 336 Cuts at: 1278 222 Size: 3083 Fragments arranged by size: 3083 336 189 174 105 87 61 57 47 EcoRI G'AATT_C Cuts at: 1143 1143 Size: 4139 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 147 489 2158 147 Size: 342 1669 2128 Fragments arranged by size: 2128 1669 342 FspI TGC'GCA Cuts at: 1920 1920 Size: 4139 HaeII r_GCGC'y Cuts at: 968 1573 1821 2843 3822 968 Size: 605 248 1022 979 1285 Fragments arranged by size: 1285 1022 979 605 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 685 717 745 777 811 843 1981 2011 2013 Size: 32 28 32 34 32 1138 30 2 Cuts at: 2013 2043 685 Size: 30 2781 Fragments arranged by size: 2781 1138 34 32 32 32 30 30 28 2 HincII GTy'rAC Cuts at: 1178 1178 Size: 4139 KasI G'GCGC_C Cuts at: 1569 1817 1569 Size: 248 3891 KpnI G_GTAC'C Cuts at: 1159 1159 Size: 4139 MluI A'CGCG_T Cuts at: 3572 3572 Size: 4139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 134 2810 2994 134 Size: 2676 184 1279 Fragments arranged by size: 2676 1279 184 MscI TGG'CCA Cuts at: 1900 1900 Size: 4139 MslI CAynn'nnrTG Cuts at: 796 2128 2255 796 Size: 1332 127 2680 Fragments arranged by size: 2680 1332 127 MspA1I CmG'CkG Cuts at: 1075 1250 1564 1924 2937 3182 1075 Size: 175 314 360 1013 245 2032 Fragments arranged by size: 2032 1013 360 314 245 175 NaeI GCC'GGC Cuts at: 2320 2320 Size: 4139 NarI GG'CG_CC Cuts at: 1570 1818 1570 Size: 248 3891 NgoMIV G'CCGG_C Cuts at: 2318 2318 Size: 4139 NspI r_CATG'y Cuts at: 1192 1692 2599 1192 Size: 500 907 2732 Fragments arranged by size: 2732 907 500 PasI CC'CwG_GG Cuts at: 851 851 Size: 4139 PciI A'CATG_T Cuts at: 2595 2595 Size: 4139 PmeI GTTT'AAAC Cuts at: 1138 1138 Size: 4139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3278 3310 3278 Size: 32 4107 PpuMI rG'GwC_Cy Cuts at: 222 411 516 222 Size: 189 105 3845 Fragments arranged by size: 3845 189 105 PspOMI G'GGCC_C Cuts at: 350 895 350 Size: 545 3594 PspXI vC'TCGA_Gb Cuts at: 325 607 325 Size: 282 3857 PstI C_TGCA'G Cuts at: 594 1186 1871 594 Size: 592 685 2862 Fragments arranged by size: 2862 685 592 PvuI CG_AT'CG Cuts at: 104 104 Size: 4139 PvuII CAG'CTG Cuts at: 1075 1564 1924 1075 Size: 489 360 3290 Fragments arranged by size: 3290 489 360 RsrII CG'GwC_CG Cuts at: 2334 2334 Size: 4139 SacI G_AGCT'C Cuts at: 932 1153 932 Size: 221 3918 SalI G'TCGA_C Cuts at: 1176 1176 Size: 4139 SapI GCTCTTCn'nnn_ Cuts at: 2162 2372 2162 Size: 210 3929 SbfI CC_TGCA'GG Cuts at: 1186 1186 Size: 4139 ScaI AGT'ACT Cuts at: 3656 3656 Size: 4139 SfcI C'TryA_G Cuts at: 33 590 1182 1867 2860 3051 33 Size: 557 592 685 993 191 1121 Fragments arranged by size: 1121 993 685 592 557 191 SfoI GGC'GCC Cuts at: 1571 1819 1571 Size: 248 3891 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4139 SmaI CCC'GGG Cuts at: 349 1161 349 Size: 812 3327 SmlI C'TyrA_G Cuts at: 325 463 607 1294 2209 2701 2963 325 Size: 138 144 687 915 492 262 1501 Fragments arranged by size: 1501 915 687 492 262 144 138 SpeI A'CTAG_T Cuts at: 67 67 Size: 4139 SphI G_CATG'C Cuts at: 1192 1692 1192 Size: 500 3639 StyI C'CwwG_G Cuts at: 173 500 764 1273 1627 173 Size: 327 264 509 354 2685 Fragments arranged by size: 2685 509 354 327 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 316 808 952 1722 1855 2314 2523 316 Size: 492 144 770 133 459 209 1932 Fragments arranged by size: 1932 770 492 459 209 144 133 TatI w'GTAC_w Cuts at: 188 3654 188 Size: 3466 673 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3656 3688 3656 Size: 32 4107 Tth111I GACn'n_nGTC Cuts at: 1936 1936 Size: 4139 XbaI T'CTAG_A Cuts at: 1170 1170 Size: 4139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1634 1634 Size: 4139 XhoI C'TCGA_G Cuts at: 325 607 325 Size: 282 3857 XmaI C'CCGG_G Cuts at: 347 1159 347 Size: 812 3327 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI