Restriction map for pF1KB9914
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BsaBI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BspEI       BspHI       
BsrBI       BsrDI       BsrI        BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoT22I     
FseI        FspI        HaeII       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        PvuII       RcaI        SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AocI          702  CCTCAGG              CC^T[ACGT]AGG
Asp700I      1300  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI           4  GGATCC               G^GATCC
BanI         1314  GGCACC               G^G[CT][GA]CC
BanII         619  GAGCCC               G[GA]GC[CT]^C
BbsI         1515  GAAGAC               GAAGAC(2/6)
BclI         1067  TGATCA               T^GATCA
BcnI          889  CCCGG                CC^[GC]GG
BfaI          370  CTAG                 C^TAG
BlpI          633  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      633  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1515  GAAGAC               GAAGAC(2/6)
BsaAI         903  CACGTA               [CT]AC^GT[GA]
BsiEI        1284  CGACCG               CG[GA][CT]^CG
BsmFI         677  GGGAC                GGGAC(10/14)
Bsp1407I      791  TGTACA               T^GTACA
BspDI          49  ATCGAT               AT^CGAT
BspLU11I      650  ACATGT               A^CATGT
BsrGI         791  TGTACA               T^GTACA
Bsu36I        702  CCTCAGG              CC^T[ACGT]AGG
CelII         633  GCTGAGC              GC^T[ACGT]AGC
ClaI           49  ATCGAT               AT^CGAT
CpoI          935  CGGTCCG              CG^G[AT]CCG
DraII         581  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI          172  GACGCCATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1411  CTCTTC               CTCTTC(1/4)
Eco81I        702  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      581  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         207  GAATTC               G^AATTC
FbaI         1067  TGATCA               T^GATCA
HincII        519  GTTGAC               GT[CT]^[GA]AC
HindII        519  GTTGAC               GT[CT]^[GA]AC
Ksp632I      1411  CTCTTC               CTCTTC(1/4)
MaeI          370  CTAG                 C^TAG
MluI          378  ACGCGT               A^CGCGT
MslI         1086  CACTGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          889  CCCGG                CC^[GC]GG
PleI          522  GACTC                GAGTC(4/5)
RsrII         935  CGGTCCG              CG^G[AT]CCG
SapI         1410  GCTCTTC              GCTCTTC(1/4)
ScaI           83  AGTACT               AGT^ACT
SmlI          312  CTCAAG               C^T[CT][GA]AG
SspBI         791  TGTACA               T^GTACA
SspI         1268  AATATT               AAT^ATT
StuI         1302  AGGCCT               AGG^CCT
XcmI           95  CCAGCGACATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1300  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AatII         604  GACGTC               GACGT^C
             1530  GACGTC              
AcsI          207  GAATTC               [GA]^AATT[CT]
             1140  GAATTT              
ApoI          207  GAATTC               [GA]^AATT[CT]
             1140  GAATTT              
AseI          273  ATTAAT               AT^TAAT
             1074  ATTAAT              
AsnI          273  ATTAAT               AT^TAAT
             1074  ATTAAT              
AspHI         646  GAGCAC               G[AT]GC[AT]^C
             1162  GAGCAC              
AvaI          664  CTCGGG               C^[CT]CG[GA]G
             1457  CCCGAG              
BalI          242  TGGCCA               TGG^CCA
             1082  TGGCCA              
BsiHKAI       646  GAGCAC               G[AT]GC[AT]^C
             1162  GAGCAC              
BsmI           59  GCATTC               GAATGC(1/-1)
              319  GCATTC              
BsoBI         664  CTCGGG               C^[CT]CG[GA]G
             1457  CCCGAG              
BspMI         557  ACCTGC               ACCTGC(4/8)
              294  GCAGGT              
BsrFI          23  ACCGGC               [GA]^CCGG[CT]
              127  ACCGGC              
BstAPI         80  GCAAGTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1096  GCAAGACATGC         
BstF5I        886  CATCC                GGATG(2/0)
             1029  CATCC               
BstXI          35  CCATCTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1085  CCACTGACATGG        
CfoI          292  GCGC                 GCG^C
              977  GCGC                
Cfr10I         23  ACCGGC               [GA]^CCGG[CT]
              127  ACCGGC              
EcoRV         841  GATATC               GAT^ATC
             1204  GATATC              
FauI          202  CCCGC                CCCGC(4/6)
              920  CCCGC               
FokI          886  CATCC                GGATG(9/13)
             1029  CATCC               
HhaI          292  GCGC                 GCG^C
              977  GCGC                
HinP1I        292  GCGC                 G^CGC
              977  GCGC                
HphI          698  TCACC                GGTGA(8/7)
              955  TCACC               
MluNI         242  TGGCCA               TGG^CCA
             1082  TGGCCA              
MscI          242  TGGCCA               TGG^CCA
             1082  TGGCCA              
MspA1I        860  CAGCGG               C[AC]G^C[GT]G
              958  CCGCTG              
NcoI          176  CCATGG               C^CATGG
             1418  CCATGG              
NspI          650  ACATGT               [GA]CATG^[CT]
             1101  ACATGC              
PflMI         873  CCACGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1033  CCACCTGATGG         
PshBI         273  ATTAAT               AT^TAAT
             1074  ATTAAT              
PstI          810  CTGCAG               CTGCA^G
             1438  CTGCAG              
TfiI         1117  GATTC                G^A[AT]TC
             1453  GAATC               
Van91I        873  CCACGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1033  CCACCTGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AvaII       BbvI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BssKI       
BstNI       BstUI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HgaI        
Hin1I       HinfI       HpaII       ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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