Restriction map for pF1KB9914
 (Linear) (Six-base) MAPSORT of: KIBB9914.seq  Check: 3115  from: 1  to: 1599



KIBB9914.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AatII G_ACGT'C

Cuts at:      0     608    1534    1599 
   Size:        608     926      65

  Fragments arranged by size: 

                 926     608      65
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     365     666    1198    1498    1599 
   Size:         60     305     301     532     300     101

  Fragments arranged by size: 

                 532     305     301     300     101      60
 
AflIII A'CryG_T

Cuts at:      0     378     650     902    1599 
   Size:        378     272     252     697

  Fragments arranged by size: 

                 697     378     272     252
 
ApoI r'AATT_y

Cuts at:      0     207    1140    1599 
   Size:        207     933     459

  Fragments arranged by size: 

                 933     459     207
 
AseI AT'TA_AT

Cuts at:      0     274    1075    1599 
   Size:        274     801     524

  Fragments arranged by size: 

                 801     524     274
 
AvaI C'yCGr_G

Cuts at:      0     664    1457    1599 
   Size:        664     793     142

  Fragments arranged by size: 

                 793     664     142
 
BamHI G'GATC_C

Cuts at:      0       4    1599 
   Size:          4    1595
 
BanI G'GyrC_C

Cuts at:      0    1314    1599 
   Size:       1314     285
 
BanII G_rGCy'C

Cuts at:      0     623    1599 
   Size:        623     976
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1522    1599 
   Size:       1522      77
 
BclI T'GATC_A

Cuts at:      0    1067    1599 
   Size:       1067     532
 
BlpI GC'TnA_GC

Cuts at:      0     634    1599 
   Size:        634     965
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     327     846    1599 
   Size:        327     519     753

  Fragments arranged by size: 

                 753     519     327
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     551     664     802     982    1599 
   Size:         78     473     113     138     180     617

  Fragments arranged by size: 

                 617     473     180     138     113      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297    1599 
   Size:        297    1302
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     186     228     565    1599 
   Size:        186      42     337    1034

  Fragments arranged by size: 

                1034     337     186      42
 
BsaAI yAC'GTr

Cuts at:      0     905    1599 
   Size:        905     694
 
BsaHI Gr'CG_yC

Cuts at:      0     173     605    1493    1531    1599 
   Size:        173     432     888      38      68

  Fragments arranged by size: 

                 888     432     173      68      38
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      76     106     637     667    1006    1036    1599 
   Size:         76      30     531      30     339      30     563

  Fragments arranged by size: 

                 563     531     339      76      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3     463     515    1174    1441    1486    1599 
   Size:          3     460      52     659     267      45     113

  Fragments arranged by size: 

                 659     460     267     113      52      45       3
 
BsiEI CG_ry'CG

Cuts at:      0    1287    1599 
   Size:       1287     312
 
BsiHKAI G_wGCw'C

Cuts at:      0     650    1166    1599 
   Size:        650     516     433

  Fragments arranged by size: 

                 650     516     433
 
BsmI GAATG_Cn'

Cuts at:      0      59     319    1599 
   Size:         59     260    1280

  Fragments arranged by size: 

                1280     260      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     623     650    1166    1599 
   Size:        623      27     516     433

  Fragments arranged by size: 

                 623     516     433      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     285     566    1599 
   Size:        285     281    1033

  Fragments arranged by size: 

                1033     285     281
 
BsrFI r'CCGG_y

Cuts at:      0      23     127    1599 
   Size:         23     104    1472

  Fragments arranged by size: 

                1472     104      23
 
BsrGI T'GTAC_A

Cuts at:      0     791    1599 
   Size:        791     808
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      86    1102    1599 
   Size:         86    1016     497

  Fragments arranged by size: 

                1016     497      86
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      42    1092    1599 
   Size:         42    1050     507

  Fragments arranged by size: 

                1050     507      42
 
BstYI r'GATC_y

Cuts at:      0       4     992    1052    1599 
   Size:          4     988      60     547

  Fragments arranged by size: 

                 988     547      60       4
 
Bsu36I CC'TnA_GG

Cuts at:      0     703    1599 
   Size:        703     896
 
BtgI C'CryG_G

Cuts at:      0     176     980    1418    1599 
   Size:        176     804     438     181

  Fragments arranged by size: 

                 804     438     181     176
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     105     548    1599 
   Size:        105     443    1051

  Fragments arranged by size: 

                1051     443     105
 
BtsI GCAGTG_nn'

Cuts at:      0     765     972    1599 
   Size:        765     207     627

  Fragments arranged by size: 

                 765     627     207
 
ClaI AT'CG_AT

Cuts at:      0      50    1599 
   Size:         50    1549
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     178    1599 
   Size:        178    1421
 
EaeI y'GGCC_r

Cuts at:      0     242     458     863    1082    1599 
   Size:        242     216     405     219     517

  Fragments arranged by size: 

                 517     405     242     219     216
 
EarI CTCTTCn'nnn_

Cuts at:      0    1417    1599 
   Size:       1417     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60      78     365     551     664     666     802     982 
   Size:         60      18     287     186     113       2     136     180

Cuts at:    982    1198    1498    1599 
   Size:        216     300     101

  Fragments arranged by size: 

                 300     287     216     186     180     136     113     101
                  60      18       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     582    1599 
   Size:        582    1017
 
EcoRI G'AATT_C

Cuts at:      0     207    1599 
   Size:        207    1392
 
EcoRV GAT'ATC

Cuts at:      0     843    1206    1599 
   Size:        843     363     393

  Fragments arranged by size: 

                 843     393     363
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      90     615    1599 
   Size:         90     525     984

  Fragments arranged by size: 

                 984     525      90
 
HincII GTy'rAC

Cuts at:      0     521    1599 
   Size:        521    1078
 
MluI A'CGCG_T

Cuts at:      0     378    1599 
   Size:        378    1221
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1049    1080    1599 
   Size:       1049      31     519

  Fragments arranged by size: 

                1049     519      31
 
MscI TGG'CCA

Cuts at:      0     244    1084    1599 
   Size:        244     840     515

  Fragments arranged by size: 

                 840     515     244
 
MslI CAynn'nnrTG

Cuts at:      0    1090    1599 
   Size:       1090     509
 
MspA1I CmG'CkG

Cuts at:      0     862     960    1599 
   Size:        862      98     639

  Fragments arranged by size: 

                 862     639      98
 
NcoI C'CATG_G

Cuts at:      0     176    1418    1599 
   Size:        176    1242     181

  Fragments arranged by size: 

                1242     181     176
 
NspI r_CATG'y

Cuts at:      0     654    1105    1599 
   Size:        654     451     494

  Fragments arranged by size: 

                 654     494     451
 
PciI A'CATG_T

Cuts at:      0     650    1599 
   Size:        650     949
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     879    1039    1599 
   Size:        879     160     560

  Fragments arranged by size: 

                 879     560     160
 
PfoI T'CCnGG_A

Cuts at:      0     818    1599 
   Size:        818     781
 
PstI C_TGCA'G

Cuts at:      0     814    1442    1599 
   Size:        814     628     157

  Fragments arranged by size: 

                 814     628     157
 
RsrII CG'GwC_CG

Cuts at:      0     936    1599 
   Size:        936     663
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1417    1599 
   Size:       1417     182
 
ScaI AGT'ACT

Cuts at:      0      85    1599 
   Size:         85    1514
 
SfcI C'TryA_G

Cuts at:      0     735     810    1149    1438    1599 
   Size:        735      75     339     289     161

  Fragments arranged by size: 

                 735     339     289     161      75
 
SmlI C'TyrA_G

Cuts at:      0     312    1599 
   Size:        312    1287
 
SspI AAT'ATT

Cuts at:      0    1270    1599 
   Size:       1270     329
 
StuI AGG'CCT

Cuts at:      0    1304    1599 
   Size:       1304     295
 
StyI C'CwwG_G

Cuts at:      0      29     176    1243    1343    1418    1599 
   Size:         29     147    1067     100      75     181

  Fragments arranged by size: 

                1067     181     147     100      75      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1236    1343    1599 
   Size:       1236     107     256

  Fragments arranged by size: 

                1236     256     107
 
TatI w'GTAC_w

Cuts at:      0      83     491     791    1599 
   Size:         83     408     300     808

  Fragments arranged by size: 

                 808     408     300      83
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     386     418    1599 
   Size:        386      32    1181

  Fragments arranged by size: 

                1181     386      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     102    1599 
   Size:        102    1497
 
XmnI GAAnn'nnTTC

Cuts at:      0    1304    1599 
   Size:       1304     295
 
ZraI GAC'GTC

Cuts at:      0     606    1532    1599 
   Size:        606     926      67

  Fragments arranged by size: 

                 926     606      67
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbsI     BclI     BlpI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DrdI     EaeI     EarI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI   HincII     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NspI     PciI    PflMI     PfoI     PstI    RsrII
     SapI     ScaI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BsaBI    BsaWI
    BseYI     BsgI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI
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