Restriction map for pF1KB9909
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BpmI        BpuAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MslI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcyI           30  GGCGTC               G[GA]^CG[CT]C
BalI          458  TGGCCA               TGG^CCA
BamHI         725  GGATCC               G^GATCC
BcgI          590  GCATCTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI         715  TACGTG               [CT]AC^GT[GA]
BsaHI          30  GGCGTC               G[GA]^CG[CT]C
BseRI         358  CTCCTC               GAGGAG(10/8)
BsiEI         203  CGGCCG               CG[GA][CT]^CG
BsmAI         602  GAGAC                GTCTC(1/5)
BsrBI          57  GAGCGG               CCGCTC(-3/-3)
BssSI          94  CACGAG               CACGAG(-5/-1)
BstEII        531  GGTCACC              G^GT[ACGT]ACC
BstPI         531  GGTCACC              G^GT[ACGT]ACC
BstYI         725  GGATCC               [GA]^GATC[CT]
DraII         503  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EagI          203  CGGCCG               C^GGCCG
EclXI         203  CGGCCG               C^GGCCG
Eco52I        203  CGGCCG               C^GGCCG
EcoO109I      503  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       531  GGTCACC              G^GT[ACGT]ACC
HaeII         233  AGCGCC               [GA]GCGC^[CT]
Hin1I          30  GGCGTC               G[GA]^CG[CT]C
MflI          725  GGATCC               [GA]^GATC[CT]
MluNI         458  TGGCCA               TGG^CCA
MscI          458  TGGCCA               TGG^CCA
NcoI          461  CCATGG               C^CATGG
PpuMI         503  AGGTCCT              [GA]G^G[AT]CC[CT]
SacI          514  GAGCTC               GAGCT^C
SbfI          507  CCTGCAGG             CCTGCA^GG
SmaI          150  CCCGGG               CCC^GGG
Sse8387I      507  CCTGCAGG             CCTGCA^GG
XcmI           17  CCAGCCCTGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         725  GGATCC               [GA]^GATC[CT]
XmaCI         150  CCCGGG               C^CCGGG
XmaI          150  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspHI         250  GTGCTC               G[AT]GC[AT]^C
              514  GAGCTC              
AvaI           68  CCCGAG               C^[CT]CG[GA]G
              150  CCCGGG              
AvaII         426  GGACC                G^G[AT]CC
              504  GGTCC               
BlpI           54  GCTGAGC              GC^T[ACGT]AGC
              369  GCTGAGC             
Bpu1102I       54  GCTGAGC              GC^T[ACGT]AGC
              369  GCTGAGC             
BsiHKAI       250  GTGCTC               G[AT]GC[AT]^C
              514  GAGCTC              
BsmFI         221  GGGAC                GGGAC(10/14)
              571  GTCCC               
BsoBI          68  CCCGAG               C^[CT]CG[GA]G
              150  CCCGGG              
BstAPI        210  GCACTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              446  GCACCCAGTGC         
CelII          54  GCTGAGC              GC^T[ACGT]AGC
              369  GCTGAGC             
DraIII        262  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              447  CACCCAGTG           
HinfI         604  GACTC                G^A[ACGT]TC
              649  GACTC               
MaeIII        523  GTCAC                ^GT[ACGT]AC
              532  GTCAC               
MboII         539  TCTTC                GAAGA(8/7)
              695  TCTTC               
MspA1I        367  CAGCTG               C[AC]G^C[GT]G
              412  CAGCTG              
NaeI          545  GCCGGC               GCC^GGC
              586  GCCGGC              
NgoMIV        545  GCCGGC               G^CCGGC
              586  GCCGGC              
NlaIII        123  CATG                 CATG^
              462  CATG                
PleI          604  GACTC                GAGTC(4/5)
              649  GACTC               
PstI          133  CTGCAG               CTGCA^G
              508  CTGCAG              
PvuII         367  CAGCTG               CAG^CTG
              412  CAGCTG              
SfcI          133  CTGCAG               C^T[GA][CT]AG
              508  CTGCAG              
Tsp45I        523  GTCAC                ^GT[GC]AC
              532  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       HphI        
ItaI        MaeII       MboI        MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       PflMI       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        TspRI       Van91I      
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