Restriction map for pF1KB9909
 (Linear) (Six-base) MAPSORT of: KIBB9909.seq  Check: 9109  from: 1  to: 768



KIBB9909.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     237     456     507     768 
   Size:        237     219      51     261

  Fragments arranged by size: 

                 261     237     219      51
 
AvaI C'yCGr_G

Cuts at:      0      68     150     768 
   Size:         68      82     618

  Fragments arranged by size: 

                 618      82      68
 
BamHI G'GATC_C

Cuts at:      0     725     768 
   Size:        725      43
 
BanI G'GyrC_C

Cuts at:      0     172     445     481     768 
   Size:        172     273      36     287

  Fragments arranged by size: 

                 287     273     172      36
 
BanII G_rGCy'C

Cuts at:      0     261     284     518     768 
   Size:        261      23     234     250

  Fragments arranged by size: 

                 261     250     234      23
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     579     613     768 
   Size:        579      34     155

  Fragments arranged by size: 

                 579     155      34
 
BlpI GC'TnA_GC

Cuts at:      0      55     370     768 
   Size:         55     315     398

  Fragments arranged by size: 

                 398     315      55
 
Bme1580I G_kGCm'C

Cuts at:      0      95     440     486     768 
   Size:         95     345      46     282

  Fragments arranged by size: 

                 345     282      95      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     444     768 
   Size:        444     324
 
Bpu10I CC'TnA_GC

Cuts at:      0     308     768 
   Size:        308     460
 
BsaAI yAC'GTr

Cuts at:      0     717     768 
   Size:        717      51
 
BsaHI Gr'CG_yC

Cuts at:      0      31     768 
   Size:         31     737
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142     768 
   Size:        112      30     626

  Fragments arranged by size: 

                 626     112      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     349     768 
   Size:        349     419
 
BseYI C'CCAG_C

Cuts at:      0      16     187     230     287     378     467     495     768 
   Size:         16     171      43      57      91      89      28     273

  Fragments arranged by size: 

                 273     171      91      89      57      43      28      16
            
 
BsiEI CG_ry'CG

Cuts at:      0     206     768 
   Size:        206     562
 
BsiHKAI G_wGCw'C

Cuts at:      0     254     518     768 
   Size:        254     264     250

  Fragments arranged by size: 

                 264     254     250
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     254     261     284     440     486     518     768 
   Size:         95     159       7      23     156      46      32     250

  Fragments arranged by size: 

                 250     159     156      95      46      32      23       7
            
 
BsrBI CCG'CTC

Cuts at:      0      59     768 
   Size:         59     709
 
BsrFI r'CCGG_y

Cuts at:      0       8     545     586     768 
   Size:          8     537      41     182

  Fragments arranged by size: 

                 537     182      41       8
 
BssSI C'ACGA_G

Cuts at:      0      94     768 
   Size:         94     674
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     216     452     768 
   Size:        216     236     316

  Fragments arranged by size: 

                 316     236     216
 
BstEII G'GTnAC_C

Cuts at:      0     531     768 
   Size:        531     237
 
BstYI r'GATC_y

Cuts at:      0     725     768 
   Size:        725      43
 
BtgI C'CryG_G

Cuts at:      0     441     461     527     768 
   Size:        441      20      66     241

  Fragments arranged by size: 

                 441     241      66      20
 
BtsI GCAGTG_nn'

Cuts at:      0     210     768 
   Size:        210     558
 
DraIII CAC_nnn'GTG

Cuts at:      0     267     452     768 
   Size:        267     185     316

  Fragments arranged by size: 

                 316     267     185
 
EaeI y'GGCC_r

Cuts at:      0     203     458     547     768 
   Size:        203     255      89     221

  Fragments arranged by size: 

                 255     221     203      89
 
EagI C'GGCC_G

Cuts at:      0     203     768 
   Size:        203     565
 
EcoICRI GAG'CTC

Cuts at:      0     516     768 
   Size:        516     252
 
EcoO109I rG'GnC_Cy

Cuts at:      0     504     768 
   Size:        504     264
 
HaeII r_GCGC'y

Cuts at:      0     237     768 
   Size:        237     531
 
MscI TGG'CCA

Cuts at:      0     460     768 
   Size:        460     308
 
MspA1I CmG'CkG

Cuts at:      0     369     414     768 
   Size:        369      45     354

  Fragments arranged by size: 

                 369     354      45
 
NaeI GCC'GGC

Cuts at:      0     547     588     768 
   Size:        547      41     180

  Fragments arranged by size: 

                 547     180      41
 
NcoI C'CATG_G

Cuts at:      0     461     768 
   Size:        461     307
 
NgoMIV G'CCGG_C

Cuts at:      0     545     586     768 
   Size:        545      41     182

  Fragments arranged by size: 

                 545     182      41
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     237     456     467     768 
   Size:        237     219      11     301

  Fragments arranged by size: 

                 301     237     219      11
 
PpuMI rG'GwC_Cy

Cuts at:      0     504     768 
   Size:        504     264
 
PstI C_TGCA'G

Cuts at:      0     137     512     768 
   Size:        137     375     256

  Fragments arranged by size: 

                 375     256     137
 
PvuII CAG'CTG

Cuts at:      0     369     414     768 
   Size:        369      45     354

  Fragments arranged by size: 

                 369     354      45
 
SacI G_AGCT'C

Cuts at:      0     518     768 
   Size:        518     250
 
SbfI CC_TGCA'GG

Cuts at:      0     512     768 
   Size:        512     256
 
SfcI C'TryA_G

Cuts at:      0     133     508     768 
   Size:        133     375     260

  Fragments arranged by size: 

                 375     260     133
 
SmaI CCC'GGG

Cuts at:      0     152     768 
   Size:        152     616
 
StyI C'CwwG_G

Cuts at:      0     461     639     755     768 
   Size:        461     178     116      13

  Fragments arranged by size: 

                 461     178     116      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     301     463     469     768 
   Size:        301     162       6     299

  Fragments arranged by size: 

                 301     299     162       6
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      24     768 
   Size:         24     744
 
XmaI C'CCGG_G

Cuts at:      0     150     768 
   Size:        150     618
 

 Enzymes that do cut:

    AlwNI     AvaI    BamHI     BanI    BanII     BcgI     BlpI Bme1580I
     BmrI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BsrBI    BsrFI    BssSI   BstAPI   BstEII    BstYI
     BtgI     BtsI   DraIII     EaeI     EagI  EcoICRI EcoO109I    HaeII
     MscI   MspA1I     NaeI     NcoI   NgoMIV    PflMI    PpuMI     PstI
    PvuII     SacI     SbfI     SfcI     SmaI     StyI    TaqII     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaWI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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