Restriction map for pF1KB7973
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BamHI       
BanII       BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BpmI        BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MamI        MflI        MluI        
MroI        MseI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PstI        PvuI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccII         142  CGCG                 CG^CG
AflIII        467  ACGTGT               A^C[GA][CT]GT
AlwI          563  GGATC                GGATC(4/5)
AspHI         571  GAGCAC               G[AT]GC[AT]^C
AvaI           22  CCCGAG               C^[CT]CG[GA]G
BalI          524  TGGCCA               TGG^CCA
BanI           13  GGCGCC               G^G[CT][GA]CC
BbeI           13  GGCGCC               GGCGC^C
BbsI          553  GTCTTC               GAAGAC(2/6)
BbvI          228  GCAGC                GCAGC(8/12)
BcgI          263  GCAAAGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI           10  CCGGG                CC^[GC]GG
BmyI          571  GAGCAC               G[AGT]GC[ACT]^C
BpuAI         553  GTCTTC               GAAGAC(2/6)
BsaI          444  GGTCTC               GGTCTC(1/5)
BsgI          616  CTGCAC               GTGCAG(16/14)
BsiHKAI       571  GAGCAC               G[AT]GC[AT]^C
BsmAI         445  GTCTC                GTCTC(1/5)
BsoBI          22  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      571  GAGCAC               G[AGT]GC[ACT]^C
BspHI         377  TCATGA               T^CATGA
BsrFI         131  ACCGGC               [GA]^CCGG[CT]
BsrI          498  CCAGT                ACTGG(1/-1)
BssHII        141  GCGCGC               G^CGCGC
BstF5I        357  GGATG                GGATG(2/0)
BstUI         142  CGCG                 CG^CG
BstXI         515  CCATCTCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        131  ACCGGC               [GA]^CCGG[CT]
DraII          26  AGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI           28  GACCCTTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          524  TGGCCA               [CT]^GGCC[GA]
EarI          165  GAAGAG               CTCTTC(1/4)
EcoO109I       26  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        31  CCTTGG               C^C[AT][AT]GG
FauI          594  GCGGG                CCCGC(4/6)
FokI          357  GGATG                GGATG(9/13)
HaeII          13  GGCGCC               [GA]GCGC^[CT]
HphI          362  TCACC                GGTGA(8/7)
KasI           13  GGCGCC               G^GCGCC
Ksp632I       165  GAAGAG               CTCTTC(1/4)
MfeI          622  CAATTG               C^AATTG
MluNI         524  TGGCCA               TGG^CCA
MscI          524  TGGCCA               TGG^CCA
MspA1I        558  CAGCTG               C[AC]G^C[GT]G
MunI          622  CAATTG               C^AATTG
MvnI          142  CGCG                 CG^CG
NarI           13  GGCGCC               GG^CGCC
NciI           10  CCGGG                CC^[GC]GG
PleI          118  GAGTC                GAGTC(4/5)
PpuMI          26  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         207  GACCATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         558  CAGCTG               CAG^CTG
RcaI          377  TCATGA               T^CATGA
SfaNI         479  GCATC                GCATC(5/9)
SfcI           54  CTACAG               C^T[GA][CT]AG
SfoI           13  GGCGCC               GGC^GCC
StuI          107  AGGCCT               AGG^CCT
StyI           31  CCTTGG               C^C[AT][AT]GG
TaqI          566  TCGA                 T^CGA
TfiI          487  GATTC                G^A[AT]TC
TseI          228  GCAGC                G^C[AT]GC
Tsp45I        361  GTCAC                ^GT[GC]AC
Tsp509I       623  AATT                 ^AATT
TthHB8I       566  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         535  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              586  CAGACCCTG           
AspI          439  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              451  GACCAGGTC           
BlpI          157  GCTGAGC              GC^T[ACGT]AGC
              258  GCTCAGC             
Bpu1102I      157  GCTGAGC              GC^T[ACGT]AGC
              258  GCTCAGC             
BsaJI          23  CCGAGG               C^C[ACGT][ACGT]GG
               31  CCTTGG              
BsmFI         173  GGGAC                GGGAC(10/14)
              214  GTCCC               
CelII         157  GCTGAGC              GC^T[ACGT]AGC
              258  GCTCAGC             
DpnII         375  GATC                 ^GATC
              564  GATC                
HaeIII        108  GGCC                 GG^CC
              525  GGCC                
HapII          10  CCGG                 C^CGG
              132  CCGG                
HgaI          175  GACGC                GACGC(5/10)
              611  GCGTC               
HinfI         118  GAGTC                G^A[ACGT]TC
              487  GATTC               
HpaII          10  CCGG                 C^CGG
              132  CCGG                
MaeII         346  ACGT                 A^CGT
              467  ACGT                
MaeIII        348  GTTAC                ^GT[ACGT]AC
              361  GTCAC               
MboI          375  GATC                 ^GATC
              564  GATC                
MslI          378  CATGAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              516  CATCTCAGTG          
MspI           10  CCGG                 C^CGG
              132  CCGG                
MwoI           59  GCGACAACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              573  GCACTGTAAGC         
NdeII         375  GATC                 ^GATC
              564  GATC                
PflFI         439  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              451  GACCAGGTC           
Sau3AI        375  GATC                 ^GATC
              564  GATC                
SexAI         440  ACCAGGT              A^CC[AT]GGT
              452  ACCAGGT             
Tth111I       439  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              451  GACCAGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BsaHI       
BseRI       BsiYI       BslI        BssKI       BstNI       
Cac8I       CfoI        Cfr13I      DdeI        EcoRII      
Fnu4HI      HhaI        Hin1I       HinP1I      ItaI        
MboII       MnlI        MvaI        NlaIII      NlaIV       
Sau96I      ScrFI       TspRI       
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