Restriction map for pF1KB7959
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstYI       BstZ17I     
ClaI        CpoI        DraI        DrdI        Eam1105I    
EarI        Eco47III    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MflI        MroI        MseI        MunI        
NarI        NcoI        NdeI        NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII        127  ACGCGT               A^C[GA][CT]GT
AocI           22  CCTCAGG              CC^T[ACGT]AGG
Asp700I        12  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          550  CCCGAG               C^[CT]CG[GA]G
BalI          350  TGGCCA               TGG^CCA
BanI          679  GGCACC               G^G[CT][GA]CC
BanII         228  GGGCTC               G[GA]GC[CT]^C
BbsI          733  GAAGAC               GAAGAC(2/6)
BglI          212  GCCCGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           72  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       72  GCTCAGC              GC^T[ACGT]AGC
BpuAI         733  GAAGAC               GAAGAC(2/6)
BsoBI         550  CCCGAG               C^[CT]CG[GA]G
BsrBI         545  CCGCTC               CCGCTC(-3/-3)
BsrI          353  CCAGT                ACTGG(1/-1)
BstEII        540  GGTCACC              G^GT[ACGT]ACC
BstPI         540  GGTCACC              G^GT[ACGT]ACC
Bsu36I         22  CCTCAGG              CC^T[ACGT]AGG
CelII          72  GCTCAGC              GC^T[ACGT]AGC
DpnII         441  GATC                 ^GATC
DraIII        379  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          215  CGGCCG               C^GGCCG
EclXI         215  CGGCCG               C^GGCCG
Eco52I        215  CGGCCG               C^GGCCG
Eco81I         22  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       540  GGTCACC              G^GT[ACGT]ACC
FauI          168  GCGGG                CCCGC(4/6)
HinfI         271  GACTC                G^A[ACGT]TC
MaeII         116  ACGT                 A^CGT
MboI          441  GATC                 ^GATC
MboII         733  GAAGA                GAAGA(8/7)
MluI          127  ACGCGT               A^CGCGT
MluNI         350  TGGCCA               TGG^CCA
MscI          350  TGGCCA               TGG^CCA
NdeII         441  GATC                 ^GATC
PleI          271  GACTC                GAGTC(4/5)
Sau3AI        441  GATC                 ^GATC
SmlI          328  CTCAAG               C^T[CT][GA]AG
TspRI         354  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          765  CCAGGAGGATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           12  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          256  AAATTC               [GA]^AATT[CT]
              295  GAATTT              
AfaI          318  GTAC                 GT^AC
              390  GTAC                
ApoI          256  AAATTC               [GA]^AATT[CT]
              295  GAATTT              
AspHI         112  GAGCAC               G[AT]GC[AT]^C
              496  GAGCAC              
AvaII         179  GGTCC                G^G[AT]CC
              474  GGTCC               
BseRI         231  CTCCTC               GAGGAG(10/8)
              688  CTCCTC              
BsiEI         215  CGGCCG               CG[GA][CT]^CG
              279  CGACCG              
BsiHKAI       112  GAGCAC               G[AT]GC[AT]^C
              496  GAGCAC              
BsmFI         180  GTCCC                GGGAC(10/14)
              597  GTCCC               
BspMI         507  GCAGGT               ACCTGC(4/8)
              537  GCAGGT              
BstF5I        567  CATCC                GGATG(2/0)
              771  GGATG               
CfoI          674  GCGC                 GCG^C
              703  GCGC                
DsaI          120  CCGTGG               C^C[GA][CT]GG
              638  CCGTGG              
EaeI          215  CGGCCG               [CT]^GGCC[GA]
              350  TGGCCA              
EcoNI          22  CCTCAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              723  CCTCTCCAAGG         
EcoT14I       101  CCAAGG               C^C[AT][AT]GG
              728  CCAAGG              
FokI          567  CATCC                GGATG(9/13)
              771  GGATG               
HhaI          674  GCGC                 GCG^C
              703  GCGC                
HinP1I        674  GCGC                 G^CGC
              703  GCGC                
PpuMI         178  GGGTCCC              [GA]G^G[AT]CC[CT]
              473  AGGTCCT             
PvuII         592  CAGCTG               CAG^CTG
              601  CAGCTG              
RsaI          318  GTAC                 GT^AC
              390  GTAC                
StyI          101  CCAAGG               C^C[AT][AT]GG
              728  CCAAGG              
Tsp509I       257  AATT                 ^AATT
              296  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrFI       BssKI       BstNI       BstUI       
Cac8I       Cfr10I      Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HgaI        HpaII       HphI        ItaI        MaeIII      
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NgoMIV      
NlaIV       PstI        Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        Tsp45I      TthHB8I     
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